DIN EN 15851-2010 Foodstuffs - Determination of aflatoxin B in cereal based foods for infants and young children - HPLC method with immunoaffinity column cleanup and fluorescence d.pdf
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1、July 2010 Translation by DIN-Sprachendienst.English price group 11No part of this translation may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).ICS 6
2、7.060; 67.230!$izO“1708744www.din.deDDIN EN 15851Foodstuffs Determination of aflatoxin B1 in cereal based foods for infantsand young children HPLC method with immunoaffinity column cleanup and fluorescencedetectionEnglish translation of DIN EN 15851:2010-07Lebensmittel Bestimmung von Aflatoxin B1 in
3、 Suglings- und Kleinkindernahrung aufGetreidebasis HPLC-Verfahren mit Reinigung an einer Immunoaffinittssule und FluoreszenzdetektionEnglische bersetzung von DIN EN 15851:2010-07Produits alimentaires Dosage de laflatoxine B1 dans les produits pour nourrissons et jeunes enfants base de crales Mthode
4、de chromatographie liquide haute performance avec purification sur colonnedimmunoaffinit et dtection par fluorescenceTraduction anglaise de DIN EN 15851:2010-07www.beuth.deDocument comprises pagesIn case of doubt, the German-language original shall be considered authoritative.1707.10 DIN EN 15851:20
5、10-07 A comma is used as the decimal marker. National foreword This standard has been prepared by Technical Committee CEN/TC 275 “Food analysis Horizontal methods” (Secretariat: DIN, Germany). Preliminary work was done by Working Group WG 5 “Biotoxins”. The responsible German body involved in its pr
6、eparation was the Normenausschuss Lebensmittel und landwirtschaftliche Produkte (Food and Agricultural Products Standards Committee), Working Committee NA 057-01-03 AA Biotoxine. Mycotoxins are extremely toxic secondary metabolites produced by fungi. Food that is grown, harvested or stored under hum
7、id conditions can be infected by fungi whose metabolites invade the food. Because some mycotoxins are extremely toxic for humans, their reliable detection is especially important for consumer health and protection. In Germany the regulation Mykotoxin-Hchstmengenverordnung governs the maximum allowab
8、le content of mycotoxins in food. Since 2004 this regulation has contained specifications not only for aflatoxins, but also for ochratoxin A, fumonisins, deoxynivalenol (DON), and zearalenone. National regulations have been supplemented by EU-wide regulations regarding the maximum content of contami
9、nants in food since 2001. The maximum content of mycotoxins in certain foods is also laid down in various other regulations. The DIN Standard corresponding to the International Standard referred to in this document is as follows: EN ISO 3696 DIN ISO 3696 National Annex NA (informative) Bibliography
10、DIN ISO 3696, Water for analytical laboratory use Specification and test methods 2 EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN 15851 April 2010 ICS 67.060 English Version Foodstuffs - Determination of aflatoxin B1in cereal based foods for infants and young children - HPLC method with immuno
11、affinity column cleanup and fluorescence detection Produits alimentaires - Dosage de laflatoxine B1dans les produits pour nourrissons et jeunes enfants base de crales - Mthode de chromatographie liquide haute performance avec purification sur colonne dimmunoaffinit et dtection par fluorescence Leben
12、smittel - Bestimmung von Aflatoxin B1in Suglings- und Kleinkindernahrung auf Getreidebasis - HPLC-Verfahren mit Reinigung an einer Immunoaffinittssule und Fluoreszenzdetektion This European Standard was approved by CEN on 27 February 2010. CEN members are bound to comply with the CEN/CENELEC Interna
13、l Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN Management Centre or to any CEN member
14、. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the official versions. CEN members
15、 are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sw
16、eden, Switzerland and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: Avenue Marnix 17, B-1000 Brussels 2010 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members
17、. Ref. No. EN 15851:2010: EEN 15851:2010 (E) 2 Contents Page Foreword 31 Scope 42 Normative references 43 Principle 44 Reagents .45 Apparatus .76 Procedure .87 HPLC analysis 98 Calculation . 119 Precision 1110 Test report . 12Annex A (informative) Typical chromatogram 13Annex B (informative) Precisi
18、on data . 14Bibliography . 15DIN EN 15851:2010-07 EN 15851:2010 (E) 3 Foreword This document (EN 15851:2010) has been prepared by Technical Committee CEN/TC 275 “Food analysis - Horizontal methods”, the secretariat of which is held by DIN. This European Standard shall be given the status of a nation
19、al standard, either by publication of an identical text or by endorsement, at the latest by October 2010, and conflicting national standards shall be withdrawn at the latest by October 2010. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
20、 rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. This document has been prepared under a mandate given to CEN by the European Commission and the European Free Trade Association. WARNING The use of this standard can involve hazardous materials,
21、operations and equipment. This standard does not purport to address all the safety problems associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Acco
22、rding to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, It
23、aly, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and the United Kingdom. DIN EN 15851:2010-07 EN 15851:2010 (E) 4 1 Scope This European Standard specifies a method for the determination of aflatoxin B1in baby fo
24、od by high performance liquid chromatography (HPLC) with immunoaffinity cleanup and fluorescence detection. This method has been validated in an interlaboratory study via the analysis of both naturally contaminated and spiked samples ranging from 0,07 g/kg to 0,18 g/kg. For further information on th
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