DIN EN 46-2-2016 Wood preservatives - Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) - Part 2 Ovicidal effect (laboratory m.pdf

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1、November 2016 English price group 13No part of this translation may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).ICS 71.100.50!%ug“2588268www.din.de

2、DIN EN 46-2Wood preservatives Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) Part 2: Ovicidal effect (laboratory method);English version EN 462:2016,English translation of DIN EN 46-2:2016-11Holzschutzmittel Bestimmung der vorbeugenden Wirkung

3、 gegenber frisch geschlpften Larven von Hylotrupes bajulus (Linnaeus) Teil 2: Ovizide Wirkung (Laboratoriumsverfahren);Englische Fassung EN 462:2016,Englische bersetzung von DIN EN 46-2:2016-11Produits de prservation du bois Dtermination de laction prventive contre les larves rcemment closes dHylotr

4、upes bajulus (Linnaeus) Partie 2: Effet ovicide (Mthode de laboratoire);Version anglaise EN 462:2016,Traduction anglaise de DIN EN 46-2:2016-11SupersedesDIN EN 462:201003www.beuth.deDocument comprises 25 pagesDTranslation by DIN-Sprachendienst.In case of doubt, the German-language original shall be

5、considered authoritative.11.16 DIN EN 46-2:2016-11 2 A comma is used as the decimal marker. National foreword This document (EN 46-2:2016) has been prepared by Technical Committee CEN/TC 38 “Durability of wood and wood-based products” (Secretariat: AFNOR, France). The responsible German body involve

6、d in its preparation was DIN-Normenausschuss Holzwirtschaft und Mbel (DIN Standards Committee Timber and Furniture), Working Committee NA 042-03-06 AA Spiegelausschuss zu CEN/TC 38 und ISO/TC 165/SC 1 Dauerhaftigkeit von Holz und Holzprodukten. Amendments This standard differs from DIN EN 46-2:2010-

7、03 as follows: a) the requirements for wood quality have been unified; b) it is now possible to omit control test specimens treated with a solvent or diluent when the solvent or diluent is water of drinking quality. Previous editions DIN EN 46-2: 2006-06, 2010-03 EUROPEAN STANDARD NORME EUROPENNE EU

8、ROPISCHE NORM EN 46-2 June 2016 ICS 71.100.50 Supersedes EN 46-2:2009English Version Wood preservatives - Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) - Part 2: Ovicidal effect(laboratorymethod) Produits de prservation du bois - Dtermination

9、 de laction prventive contre les larves rcemment closes dHylotrupes bajulus (Linnaeus) - Partie 2: Effet ovicide (Mthode de laboratoire) Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenber frisch geschlpften Larven von Hylotrupes bajulus (Linnaeus) - Teil 2: Ovizide Wirkung (Laboratorium

10、sverfahren) This European Standard was approved by CEN on 5 January 2017. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliogr

11、aphical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibi

12、lity of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic

13、 of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey andUnited Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISAT

14、ION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels 2016 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 46-2:2016 EContents Page European foreword 4 Introduction 5 1 Scope 6 2 Normat

15、ive references 6 3 Terms and definitions . 6 4 Principle . 7 5 Test materials 7 5.1 Biological material . 7 5.2 Products and reagents 7 5.3 Apparatus 7 6 Sampling . 8 7 Test specimens 8 7.1 Species of wood 8 7.2 Wood quality 8 7.3 Provision of test specimens 9 7.4 Dimensions of test specimens 9 7.5

16、Number of test specimens 9 7.5.1 Test specimens for egg-laying 9 7.5.2 Test specimens for checking the tunnelling ability and the mortality of the larvae . 10 8 Procedure 10 8.1 Preparation of the test specimens 10 8.1.1 Conditioning of the test specimens prior to sealing . 10 8.1.2 Sealing of block

17、 faces . 10 8.1.3 Treatment of the test specimens . 10 8.1.4 Drying and conditioning of the test specimens after treatment 12 8.2 Exposure of the test specimens to the insects (ovicidal action) 12 8.3 Validity of the test . 13 9 Expression of results . 13 9.1 Ovicidal test 13 9.2 Tunnelling control

18、13 9.3 Total mortality . 14 10 Test report . 14 Annex A (informative) Example of a test report . 16 Annex B (informative) Technique for culturing Hylotrupes bajulus (Linnaeus) 18 B.1 General 18 B.2 Obtaining parent beetles 18 B.3 Mating . 18 EN 46-2:2016 (E) DIN EN 46-2:2016-11 2 B.4 Egg-Iaying 18 B

19、.5 Hatching of eggs 19 B.6 Larval development . 19 B.7 Enemies and parasites 20 Annex C (informative) Differentiation of heartwood and sapwood in Pinus species 21 C.1 Principle . 21 C.2 Reagents . 21 C.3 Apparatus 21 C.4 Procedure 21 Annex D (informative) Environmental, health and safety precautions

20、 within chemical/biological laboratory . 22 Bibliography . 23 EN 46-2:2016 (E) DIN EN 46-2:2016-11 3 European foreword This document (EN 46-2:2016) has been prepared by Technical Committee CEN/TC 38 “Durability of wood and wood-based products”, the secretariat of which is held by AFNOR. This Europea

21、n Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by December 2016, and conflicting national standards shall be withdrawn at the latest by December 2016. Attention is drawn to the possibility that some of the elem

22、ents of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. This document supersedes EN 46-2:2009. Significant technical differences between this document and EN 46-2:2009 are as follows: a) introduction o

23、f new harmonised specifications for wood quality; b) option to omit control test specimens treated with the solvent or diluents only when the solvent or diluents is water of drinking quality. The standard EN 46 is composed of two parts: EN 46-1, Wood preservatives Determination of the preventive act

24、ion against recently hatched larvae of Hylotrupes bajulus (Linnaeus) Part 1: Application by surface treatment (laboratory method) EN 46-2, Wood preservatives Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) Part 2: Ovicidal effect (laboratory me

25、thod) EN 46 consists of two parts to enable preventive action of wood preservatives, against recently hatched larvae of Hylotrupes bajulus, which are intended to be applied by surface treatment; Part 1 is required to determine the larvicidal effect of preservatives and Part 2 is required to determin

26、e the ovicidal action of the preservatives after egg-laying of young females. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic,

27、 Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. EN 46-2:2

28、016 (E) DIN EN 46-2:2016-11 4 Introduction This test method describes a laboratory method of test which gives a basis for the assessment of the preventive action of a wood preservative, when applied as a surface treatment for timber, against eggs of Hylotrupes bajulus. In combination with EN 46-1 it

29、 provides a means of checking whether larvae may hatch from eggs laid on the treated wood surface and whether they are capable of boring through the treated surface and of surviving in the untreated part of the wood. This standard provides information for the sealing of all but one lateral face when

30、 specimens are to be treated by dipping. When products which are very active at very low concentration are used, it is very important to take suitable precautions to isolate and separate, as far as possible, operations involving chemical products, other products, treated wood, laboratory apparatus a

31、nd clothing. Suitable precautions should include the use of separate rooms, areas within rooms, extraction facilities, conditioning chambers and special training for personnel (see also Annex D for environmental, health and safety precautions). EN 46-2:2016 (E) DIN EN 46-2:2016-11 5 1 Scope This Eur

32、opean Standard specifies a method for the determination of the preventive action of a wood preservative against eggs of Hylotrupes bajulus (Linnaeus) when the preservative is applied as a surface treatment to wood. This method is applicable to: water-insoluble chemicals which are being studied as ac

33、tive insecticides; organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates; organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of concentrates; or water-soluble materials, for example salts. The method is applicable

34、whether or not the test specimens have been subjected to appropriate ageing procedures. 2 Normative references The following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. Fo

35、r undated references, the latest edition of the referenced document (including any amendments) applies. EN 46-1, Wood preservatives Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) Part 1: Application by surface treatment (laboratory method) EN

36、73, Wood preservatives Accelerated ageing of treated wood prior to biological testing Evaporative ageing procedure EN 84, Wood preservatives Accelerated ageing of treated wood prior to biological testing Leaching procedure EN ISO 3696, Water for analytical laboratory use Specification and test metho

37、ds (ISO 3696) 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 representative sample sample having its physical and/or chemical characteristics identical to the volumetric average characteristics of the total volume being sampled SOURCE: EN 10

38、01-2:2005, 4.71 EN 46-2:2016 (E) DIN EN 46-2:2016-11 6 3.2 supplier sponsor of the test (person or company providing the sample of wood preservative to be tested) Note 1 to entry: Adapted from EN 10012:2005, 4.83. 4 Principle In this laboratory method treated wood panels are offered to freshly mated

39、 Hylotrupes bajulus females. The hatching ability of the larvae on the treated timber is examined. When the ovicidal action is insufficient, the mortality of the hatched larvae on and/or in wood treated with the same formulation is also established according to EN 46-1. 5 Test materials 5.1 Biologic

40、al material 5.1.1 Hylotrupes bajulus (Linnaeus) females. 5.1.2 Source of females The insects shall preferably be obtained from cultures reared as e.g. described in Annex B. Use only sound and lively insects. 5.2 Products and reagents 5.2.1 Paraffin wax, for fixing the glass plate in all cases and fo

41、r sealing the end faces of test specimens to be treated with solutions in all cases in which water is the continuous phase. NOTE Paraffin wax with a setting point of 52 C to 54 C has been found to be suitable. 5.2.2 Gelatine, for sealing the end faces of test specimens to be treated with solutions i

42、n which an organic solvent is the continuous phase. 5.2.3 Water, complying with grade 3 of EN ISO 3696. 5.2.4 Solvent or diluent, a volatile liquid that will dissolve or dilute the preservative but does not leave a residue in the wood at the end of the post-treatment conditioning period that has a t

43、oxic effect on the insects. CAUTION Do not use benzene or other solvents which pose a health risk. 5.3 Apparatus 5.3.1 Culturing chamber, with air circulation, and controlled at (28 2) C and at relative humidity (70 5) %. 5.3.2 Conditioning chamber, well ventilated and controlled at (20 2) C and at

44、relative humidity (65 5) %. The conditioning of test specimens may be carried out in the laboratory work area (see 5.3.3) provided that this has the conditions specified for the conditioning chamber (see 5.3.2). EN 46-2:2016 (E) DIN EN 46-2:2016-11 7 5.3.3 Laboratory work area, well ventilated, wher

45、e treatment of the test specimens is carried out. CAUTION It is essential to follow safety procedures for handling flammable and toxic materials. Avoid excessive exposure of operators to solvents or their vapours. 5.3.4 Testing chamber, ventilated and air-conditioned, controlled at (22 2) C and at r

46、elative humidity between (70 5) %. 5.3.5 Petri dishes of glass or polyvinylchloride (PVC), diameter ca. 9 cm for mating the insects and for egg-laying. 5.3.6 Weights, to provide ballast for the test specimens. The weights shall not react with any materials with which they come into contact during th

47、e test. 5.3.7 Safety equipment, protective clothing, appropriate for the test product and the test solvent, to ensure the safety of the operator. 5.3.8 Glass plates, (48 1) mm long and (25 1) mm wide, intended to provide a lateral slit on the test specimens. 5.3.9 Ordinary laboratory equipment, including a balance capable of weighing to an accuracy of 0,01 g and equipment for applying a liquid product by brushing or by pipette. 5.3.10 Protective gloves 6 Sampling The sample of preservative shall be representative of the product to