DIN EN 14166-2009 Foodstuffs - Determination of vitamin B by microbiological assay English version of DIN EN 14166 2009-09《食品 采用微生物法测定维生素B6 英文版本DIN EN 14166-2009-09》.pdf
《DIN EN 14166-2009 Foodstuffs - Determination of vitamin B by microbiological assay English version of DIN EN 14166 2009-09《食品 采用微生物法测定维生素B6 英文版本DIN EN 14166-2009-09》.pdf》由会员分享,可在线阅读,更多相关《DIN EN 14166-2009 Foodstuffs - Determination of vitamin B by microbiological assay English version of DIN EN 14166 2009-09《食品 采用微生物法测定维生素B6 英文版本DIN EN 14166-2009-09》.pdf(16页珍藏版)》请在麦多课文档分享上搜索。
1、September 2009DEUTSCHE NORM English price group 10No part of this standard may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).ICS 07.100.30!$YqZ“15478
2、55www.din.deDDIN EN 14166Foodstuffs Determination of vitamin B6 by microbiological assayEnglish version of DIN EN 14166:2009-09Lebensmittel Mikrobiologische Bestimmung von Vitamin B6Englische Fassung DIN EN 14166:2009-09SupersedesDIN V ENV 14166:2002-02www.beuth.deDocument comprises pages16DIN EN 14
3、166:2009-09 National foreword This standard has been prepared by Technical Committee CEN/TC 275 “Food analysis Horizontal methods” (Secretariat: DIN, Germany). Preliminary work was done by Working Group WG 9 “Vitamins and Carotenoids”. The responsible German body involved in its preparation was the
4、Normenausschuss Lebensmittel und landwirtschaftliche Produkte (Food and Agricultural Products Standards Committee), Technical Committee NA 057-01-13 AA Vitamine und Carotinoide. The DIN Standard corresponding to the International Standard referred to in this document is as follows: EN ISO 3696 DIN I
5、SO 3696 Amendments This standard differs from DIN V ENV 14166:2002-02 as follows: a) Pyridoxine hydrochloride is now used as the only standard substance for the preparation of stock and calibration solutions. b) The standard has been revised in form and substance and has been brought in line with th
6、e current rules of presentation. Previous editions DIN V ENV 14166: 2002-02 National Annex NA (informative) Bibliography DIN ISO 3696, Water for analytical laboratory use Specification and test methods 2 EUROPEAN STANDARDNORME EUROPENNEEUROPISCHE NORMEN 14166May 2009ICS 07.100.30 Supersedes ENV 1416
7、6:2001 English VersionFoodstuffs - Determination of vitamin B6 by microbiologicalassayProduits alimentaires - Dtermination de la vitamine paressai microbiologiqueLebensmittel - Mikrobiologische Bestimmung von Vitamin B6This European Standard was approved by CEN on 23 April 2009.CEN members are bound
8、 to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the CEN M
9、anagement Centre or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as
10、 theofficial versions.CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,Romania, Slovaki
11、a, Slovenia, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMIT EUROPEN DE NORMALISATIONEUROPISCHES KOMITEE FR NORMUNGManagement Centre: Avenue Marnix 17, B-1000 Brussels 2009 CEN All rights of exploitation in any form and by any means reservedworldwide for CEN
12、 national Members.Ref. No. EN 14166:2009: EB6EN 14166:2009 (E) 2 Contents Page Foreword 31 Scope 42 Normative references 43 Principle 44 Reagents .45 Apparatus .76 Procedure .77 Calculation 98 Criteria for acceptance of data 109 Precision 1110 Test Report 11Annex A (informative) Vitamin B6 calibrati
13、on lines obtained by MBA using the test organism Saccharomyces uvarum . 12Annex B (informative) Precision data . 13Bibliography . 14DIN EN 14166:2009-09 EN 14166:2009 (E) 3 Foreword This document (EN 14166:2009) has been prepared by Technical Committee CEN/TC 275 “Food analysis - Horizontal methods”
14、, the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by November 2009, and conflicting national standards shall be withdrawn at the latest by November 2009. Atte
15、ntion is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. This document supersedes ENV 14166:2001. According to the CEN/CENELEC Internal Regulations
16、, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands
17、, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and the United Kingdom. DIN EN 14166:2009-09 EN 14166:2009 (E) 4 1 Scope This European Standard specifies a method for the determination of total vitamin B6in foodstuffs by microbiological assay (MBA). Vitamin B6is d
18、etermined as the mass fraction of pyridoxine, pyridoxal and pyridoxamine, including their phosphorylated or glycosylated derivatives. It is usually expressed as milligram vitamin B6per 100 g of foodstuff. The method is applicable to samples that can be rendered homogeneous and do not contain high co
19、ncentrations of antibiotics or other interfering substances. This method has been validated in an inter-laboratory test on fortified and non-fortified samples such as wholemeal flour, milk powder, mixed vegetables and pigs liver at levels from 0,5 mg/100 g to 1,9 mg/100 g. For further information on
20、 the validation data, see Annex B. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments)
21、applies. EN ISO 3696:1995, Water for analytical laboratory use Specification and test methods (ISO 3696:1987) 3 Principle Pyridoxine, pyridoxal and/or pyridoxamine are extracted from foodstuffs by acid hydrolysis. The hydrolysis step liberates the vitamin B6vitamers from proteins and carbohydrates i
22、n the sample and hydrolyses the phosphates to the free vitamers. The total vitamin B6content in the sample extract is then determined by comparing the growth response of the assay test organism against growth obtained from a pyridoxine hydrochloride standard, see 1. 4 Reagents 4.1 General During ana
23、lysis, unless otherwise stated, use only reagents of recognised analytical grade and water of at least grade 1 according to EN ISO 3696:1995. The water used for reagent preparation shall be glass distilled. Once distilled, water shall be used within five days or discarded. 4.2 Chemicals and solution
24、s 4.2.1 Sulfuric acid solution, substance concentration c(H2SO4 ) = 0,22 mol/l 4.2.2 Sodium hydroxide solution, c(NaOH) = 4 mol/l 4.2.3 Wort agar, (Difco1)or suitable alternative) Dissolve the agar in glass distilled water according to the manufacturers instructions. Heat to boil. Dispense 5 ml aliq
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