BS 4285-3 11-1985 Microbiological examination for dairy purposes - Methods for detection and or enumeration of specific groups of microorganisms - Detection and enumeration of faec.pdf
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1、BRITISH STANDARD BS 4285-3.11: 1985 Faecal streptococci Microbiological examination for dairy purposes Part 3: Methods for detection and/or enumeration of specific groups of microorganisms Section 3.11: Detection and enumeration of faecal streptococci IMPORTANT NOTEIt is essential that Parts0 and1 a
2、nd Section2.1, which are published separately, be read in conjunction with this Section. UDC 637.1.055.07:579.8BS4285-3.11:1985 This British Standard, having been prepared under the directionof the Dairying Standards Committee, was published under the authority ofthe Board of BSI and comes intoeffec
3、t on 31 May1985 BSI 03-1999 The Committees responsible for this British Standard are shown inPart 0 The following BSI references relate to the work on this standard: Committee reference DAC/4 Draft for comment 84/52273 DC ISBN 0 580 14459 3 Amendments issued since publication Amd. No. Date of issue
4、CommentsBS4285-3.11:1985 BSI 03-1999 i Contents Page Foreword ii 1 Scope 1 2 Definition 1 3 Principle 1 4 Diluents and culture media 1 5 Apparatus 1 6 Sampling 1 7 Preparation of the test sample 1 8 Procedure 1 9 Calculation and expression of results 2 10 Test report 2 Publications referred to Insid
5、e back coverBS4285-3.11:1985 ii BSI 03-1999 Foreword This Section of BS4285 has been prepared under the direction of the Dairying Standards Committee. The method was not given in BS4285:1968 nor in Supplement No.1 (1970). A British Standard does not purport to include all the necessary provisions of
6、 a contract. Users of British Standards are responsible for their correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. Summary of pages This document comprises a front cover, an inside front cover, pages i and ii, pages1 and 2, an inside
7、back cover and a back cover. This standard has been updated (see copyright date) and may have had amendments incorporated. This will be indicated in the amendment table on theinside front cover.BS4285-3.11:1985 BSI 03-1999 1 1 Scope This Section of BS 4285 describes a method for the detection and en
8、umeration of faecal streptococci in milk and dairy products. NOTEThe titles of the publications referred to in this standard are listed on the inside back cover. 2 Definition For the purposes of this Section of BS4285, the following definition applies: faecal streptococci those bacteria that form ch
9、aracteristic colonies when grown on the selective medium under the conditions specified 3 Principle A test portion or series of decimal dilutions is mixed with a selective culture medium in Petri dishes and incubated. The numbers of characteristic colonies on selected plates are counted and the numb
10、er of faecal streptococci per millilitre or per gram of the original sample are calculated. 4 Diluents and culture medium 4.1 Diluents Use diluents described in BS4285-1.2. 4.2 Streptococcus agar 4.2.1 Composition. The composition of the streptococcus agar shall be as follows: 4.2.2 Preparation. Add
11、 the ingredients to the water and heat to boiling to dissolve. Adjust the pH so that it will be7.2 after sterilizing. Dispense in flasks in convenient volumes and sterilize for10min at1211C. Cool to about60C and add1mL of a filter-sterilized1% aqueous solution of triphenyltetrazolium chloride per100
12、mL of sterile medium. Mix to obtain uniform distribution. Cool to45C and prepare the plates. NOTEIt is recommended that a dehydrated complete medium (also known as KF streptococcus agar) be used following the manufacturers instructions. WARNING. Sodium azide is toxic and great care is required in th
13、e preparation of the medium. It also forms explosive compounds with metals and before disposal should be rendered safe by addition of excess sodium nitrite. 5 Apparatus NOTEFor details of apparatus including its preparation and sterilization, seeBS4285-1.2. 5.1 Usual microbiological laboratory appar
14、atus. 5.2 Incubator, at371C. 5.3 Waterbath, at451C. 5.4 Petri dishes. 5.5 Total delivery pipettes, of1mL nominal capacity. 5.6 Colony counting equipment. 6 Sampling Take the laboratory sample in accordance with BS4285-1.1. 7 Preparation of the test sample Prepare the test sample from the laboratory
15、sample in accordance with BS4285-1.1. 8 Procedure 8.1 Test portion and dilutions Measure the test portion and prepare serial decimal dilutions from the test sample (seeBS4285-1.1), making as many dilutions as are necessary to produce acceptable counts at two successive dilutions. 8.2 Inoculation of
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