BS PD IEC PAS 62958-2015 Clothes washing machines for household use Method for measuring the microbial contamination reduction《家用洗衣机 微生物污染减少的测量方法》.pdf

《BS PD IEC PAS 62958-2015 Clothes washing machines for household use Method for measuring the microbial contamination reduction《家用洗衣机 微生物污染减少的测量方法》.pdf》由会员分享，可在线阅读，更多相关《BS PD IEC PAS 62958-2015 Clothes washing machines for household use Method for measuring the microbial contamination reduction《家用洗衣机 微生物污染减少的测量方法》.pdf（34页珍藏版）》请在麦多课文档分享上搜索。

1、BSI Standards Publication Clothes washing machines for household use Method for measuring the microbial contamination reduction PD IEC/PAS 62958:2015National foreword This Published Document is the UK implementation of IEC/PAS 62958:2015. The UK participation in its preparation was entrusted by Tech

2、nical Committee CPL/59, Performance of household electrical appliances, to Subcommittee CPL/59/1, Dishwashers and washing machines. A list of organizations represented on this committee can be obtained on request to its secretary. This publication does not purport to include all the necessary provis

3、ions of a contract. Users are responsible for its correct application. The British Standards Institution 2015. Published by BSI Standards Limited 2015 ISBN 978 0 580 88356 9 ICS 97.060 Compliance with a British Standard cannot confer immunity from legal obligations. This Published Document was publi

4、shed under the authority of the Standards Policy and Strategy Committee on 30 April 2015. Amendments/corrigenda issued since publication Date Text affected PUBLISHED DOCUMENT PD IEC/PAS 62958:2015IEC PAS 62958 Edition 1.0 2015-03 PUBLICLY AVAILABLE SPECIFICATION PRE-STANDARD Clothes washing machines

5、 for household use Method for measuring the microbial contamination reduction INTERNATIONAL ELECTROTECHNICAL COMMISSION ICS 97.060 ISBN 978-2-8322-2478-6 Registered trademark of the International Electrotechnical Commission Warning! Make sure that you obtained this publication from an authorized dis

6、tributor. 2 IEC PAS 62958:2015 IEC 2015 CONTENTS FOREWORD . 5 INTRODUCTION . 7 1 Scope 8 2 Normative references 8 3 Terms, definitions and symbols 9 3.1 Terms and definitions 9 3.2 Symbols 9 4 Requirements 10 5 Test conditions, materials, equipment and instrumentation 10 5.1 Test conditions . 10 5.2

7、 Materials and reagents . 10 5.2.1 Microorganisms for test purposes 10 5.2.2 Culture media and solutions 10 5.2.3 Detergent 13 5.3 Equipment 14 5.3.1 General . 14 5.3.2 Incubator . 14 5.3.3 Autoclave 14 5.3.4 Microorganism carrier 14 5.3.5 Pipettes . 14 5.3.6 Electromechanical agitator. 14 5.3.7 Cen

8、trifuge, centrifuge tubes . 14 5.3.8 Base load 14 5.3.9 Measuring equipment for assessing temperature profile 15 5.3.10 Measuring equipment for water consumption . 15 6 Tests . 15 6.1 Test method principles 15 6.2 Preparation of test washing machine . 15 6.3 Preparation of test microorganisms and bi

9、o monitors 15 6.3.1 Cultures . 15 6.3.2 Bio monitors 17 6.4 Main test . 18 6.4.1 General . 18 6.4.2 Evidence of test microorganisms . 18 6.5 Validation 18 6.5.1 Enumeration of microorganisms before exposition N 0(bio monitor reference) 18 6.5.2 Negative control (cross contamination) 18 6.5.3 Determi

10、nation of water quality . 19 6.5.4 Determination of water quantity in the main wash 19 7 Evaluation . 19 7.1 Log reduction 19 7.2 Cross contamination . 19 8 Test report . 19 Annex A (informative) Microorganism reduction in household washing machines with risk class 1 test microorganisms for internal

11、 development purpose 21 PD IEC/PAS 62958:2015IEC PAS 62958:2015 IEC 2015 3 A.1 Scope . 21 A.2 General recommendation 21 A.3 Material and reagents . 21 A.3.1 Microorganisms on bio monitors 21 A.3.2 Water for culture media and solutions 21 A.3.3 Culture media and solutions 21 A.3.4 Detergent 23 A.4 Eq

12、uipment 23 A.4.1 Incubator . 23 A.4.2 Autoclave 23 A.4.3 Microorganism carrier 23 A.4.4 Pipettes . 23 A.4.5 Electromechanical agitator. 23 A.4.6 Centrifuge 23 A.4.7 Base load 23 A.4.8 Measuring equipment for assessing temperature profile 23 A.4.9 Measuring equipment for water consumption . 23 A.5 Te

13、sts 23 A.5.1 Test method principles . 23 A.5.2 Preparation of washing machine 24 A.5.3 Preparation of test organisms and bio monitors . 24 A.5.4 Main test . 25 A.5.5 Validation 26 A.6 Evaluation . 26 A.6.1 Plate counts per bio monitor 26 A.6.2 Log reductions . 27 A.6.3 Cross contamination 27 A.7 Tes

14、t report 27 Annex B (informative) Sources of materials and supplies . 28 B.1 Disclaimer . 28 B.2 Microorganism carriers . 28 B.3 Electromechanical agitator 28 B.4 Ready-to-use bio monitors 28 B.5 Nonionic surfactant and emulsifier 28 Bibliography 29 Figure A.1 Scheme for preparing a dilution series

15、25 Table 1 Composition of Tryptone Soy Agar (TSA) 11 Table 2 Composition of Sabouraud Dextrose Agar with Chloramphenicol 11 Table 3 Composition of Cetrimide Agar 11 Table 4 Composition of Baird-Parker Agar . 12 Table 5 Composition of Malt Extract Agar (MEA) 12 Table 6 Composition of Tryptone Soy Bro

16、th (TSB) . 12 Table 7 Composition of diluting agent 13 Table 8 Composition of recommended neutralisation solution 13 Table 9 Specifications for temperature logger 15 PD IEC/PAS 62958:2015 4 IEC PAS 62958:2015 IEC 2015 Table A.1 Composition of Sabouraud Dextrose Agar 22 Table A.2 Composition of Colum

17、bia CNA Agar . 22 PD IEC/PAS 62958:2015IEC PAS 62958:2015 IEC 2015 5 INTERNATIONAL ELECTROTECHNICAL COMMISSION _ CLOTHES WASHING MACHINES FOR HOUSEHOLD USE METHOD FOR MEASURING THE MICROBIAL CONTAMINATION REDUCTION FOREWORD 1) The International Electrotechnical Commission (IEC) is a worldwide organi

18、zation for standardization comprising all national electrotechnical committees (IEC National Committees). The object of IEC is to promote international co-operation on all questions concerning standardization in the electrical and electronic fields. To this end and in addition to other activities, I

19、EC publishes International Standards, Technical Specifications, Technical Reports, Publicly Available Specifications (PAS) and Guides (hereafter referred to as “IEC Publication(s)”). Their preparation is entrusted to technical committees; any IEC National Committee interested in the subject dealt wi

20、th may participate in this preparatory work. International, governmental and non- governmental organizations liaising with the IEC also participate in this preparation. IEC collaborates closely with the International Organization for Standardization (ISO) in accordance with conditions determined by

21、agreement between the two organizations. 2) The formal decisions or agreements of IEC on technical matters express, as nearly as possible, an international consensus of opinion on the relevant subjects since each technical committee has representation from all interested IEC National Committees. 3)

22、IEC Publications have the form of recommendations for international use and are accepted by IEC National Committees in that sense. While all reasonable efforts are made to ensure that the technical content of IEC Publications is accurate, IEC cannot be held responsible for the way in which they are

23、used or for any misinterpretation by any end user. 4) In order to promote international uniformity, IEC National Committees undertake to apply IEC Publications transparently to the maximum extent possible in their national and regional publications. Any divergence between any IEC Publication and the

24、 corresponding national or regional publication shall be clearly indicated in the latter. 5) IEC itself does not provide any attestation of conformity. Independent certification bodies provide conformity assessment services and, in some areas, access to IEC marks of conformity. IEC is not responsibl

25、e for any services carried out by independent certification bodies. 6) All users should ensure that they have the latest edition of this publication. 7) No liability shall attach to IEC or its directors, employees, servants or agents including individual experts and members of its technical committe

26、es and IEC National Committees for any personal injury, property damage or other damage of any nature whatsoever, whether direct or indirect, or for costs (including legal fees) and expenses arising out of the publication, use of, or reliance upon, this IEC Publication or any other IEC Publications.

27、 8) Attention is drawn to the Normative references cited in this publication. Use of the referenced publications is indispensable for the correct application of this publication. 9) Attention is drawn to the possibility that some of the elements of this IEC Publication may be the subject of patent r

28、ights. IEC shall not be held responsible for identifying any or all such patent rights. A PAS is a technical specification not fulfilling the requirements for a standard, but made available to the public. IEC PAS 62958 has been processed by subcommittee 59D: Performance of household and similar elec

29、trical laundry appliances, of IEC technical committee 59: The text of this PAS is based on the following document: This PAS was approved for publication by the P-members of the committee concerned as indicated in the following document Draft PAS Report on voting 59D/423/PAS 59D/427/RVD Following pub

30、lication of this PAS, which is a pre-standard publication, the technical committee or subcommittee concerned may transform it into an International Standard. PD IEC/PAS 62958:2015 6 IEC PAS 62958:2015 IEC 2015 This PAS shall remain valid for an initial maximum period of 3 years starting from the pub

31、lication date. The validity may be extended for a single period up to a maximum of 3 years, at the end of which it shall be published as another type of normative document, or shall be withdrawn. A bilingual version of this publication may be issued at a later date. PD IEC/PAS 62958:2015IEC PAS 6295

32、8:2015 IEC 2015 7 INTRODUCTION SC 59D decided to address the measurement of the microbial contamination reduction in washing machines by developing a globally acceptable Publicly Available Specification to respond to the increase in consumer complaints regarding odour from washing machines and washe

33、d laundry caused by presence of microorganisms. PD IEC/PAS 62958:2015 8 IEC PAS 62958:2015 IEC 2015 CLOTHES WASHING MACHINES FOR HOUSEHOLD USE METHOD FOR MEASURING THE MICROBIAL CONTAMINATION REDUCTION 1 Scope This Publicly Available Specification (PAS) specifies a test method for measuring the redu

34、ction of microbial contamination in clothes washing machines and of the possible cross contamination to uncontaminated load. NOTE A significant differentiation in microorganism contamination reduction capability of washing machines can be expected at wash temperature not higher than 40 C. This PAS a

35、pplies to clothes washing machines for household use, with or without heating devices utilising cold and/or hot water supply. It also covers washing machines which specify the use of no detergent for normal use. This PAS applies also to washing machines for communal use in blocks of flats or in laun

36、derettes. This PAS does not deal with professional washing machines nor with commercial laundry operations associated with food service, hospital linens or other non-residential applications. It also does not address the needs of persons with specific health conditions requiring special sanitization

37、 and/or disinfection techniques. This PAS does not specify safety requirements and does not deal with performance of washing machines measured under IEC 60456 nor with effects on fabrics. 2 Normative references IEC 60456:2010, Clothes washing machines for household use Methods for measuring the perf

38、ormance ISO 2267, Surface active agents Evaluation of certain effects of laundering Methods of preparation and use of unsoiled cotton control cloth EN 1276, Chemical disinfectants and antiseptics. Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and

39、antiseptics used in food, industrial, domestic and institutional areas. Test method and requirements (phase 2, step 1) EN 1650, Chemical disinfectants and antiseptics. Quantitative suspension test for the evaluation of fungicidal or yeasticidal activity of chemical disinfectants and antiseptics used

40、 in food, industrial, domestic and institutional areas. Test method and requirements (phase 2, step 1) EN 12353, Chemical disinfectants and antiseptics. Preservation of test organisms used for the determination of bactericidal (including Legionella), mycobactericidal, sporicidal, fungicidal and viru

41、cidal (including bacteriophages) activity PD IEC/PAS 62958:2015IEC PAS 62958:2015 IEC 2015 9 3 Terms, definitions and symbols 3.1 Terms and definitions 3.1.1 washing machine appliance for cleaning and rinsing of textiles using water which may also have a means of extracting excess water from the tex

42、tiles 3.1.2 test washing machine washing machine that is subjected to part or all of the requirements in this PAS in order to determine its performance 3.1.3 test run single performance assessment 3.1.4 programme series of operations which are pre-defined within the washing machine and which are dec

43、lared by the manufacturer as suitable for washing certain textile types 3.1.5 base load textile load used for testing not including the bio monitors 3.1.6 cross contamination transfer of microorganisms from fabrics to fabrics during one test run 3.1.7 inoculum quantity of cells to be inoculated in c

44、ulture medium 3.1.8 bio monitor microorganism carrier inoculated with microorganisms to be used to monitor the reduction of microbial contamination 3.1.9 temperature profile timetemperature data representing the water temperature in the washing machine during the test run 3.2 Symbols N 0average valu

45、e of microorganism amount of the two positive controls, before exposition to the test programme (cfu/bio monitor) N average value of microorganism amount per 5 bio monitors, after exposition to the test programme (cfu/bio monitor) log(N 0 /N) reduction factor per microorganism type v -1microorganism

46、 amount in a 10 times diluted solution v -2microorganism amount in a 100 times diluted solution SD initialstandard deviation of N 0SD washedstandard deviation of N PD IEC/PAS 62958:2015 10 IEC PAS 62958:2015 IEC 2015 SD totalstandard deviation of the reduction factor per microorganism type 4 Require

47、ments This Publicly Available Specification specifies a test method for measuring the reduction of microbial contamination in clothes washing machines and of the possible cross contamination in subsequent washed loads. This PAS does not specify safety requirements and does not deal with performance

48、of washing machines measured under IEC 60456 nor with effects on fabrics. 5 Test conditions, materials, equipment and instrumentation 5.1 Test conditions For all requirements regarding ambient conditions (electricity supply, water supply, ambient temperature and humidity) and base load, see IEC 6045

49、6:2010. Additionally, the water supplied to the test washing machine shall not contain more than 100 cfu/ml. Microorganisms for test purposes as listed in 5.2.1 shall not be present in the water. The micribiological quality of the water supplied to the test washing machine is determined according to 5.2.3.2 and 6.5.3 5.2 Materials and reagents 5.2.1 Microorganisms for test purposes For test purposes the following microorganisms, the first two bacteria strains and the last one a y