DB61 T 408-2007 牛奶中D-乳酸 L乳酸的测定—酶法.pdf

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1、DB61 if JF ift DB61/T408-2007 2007-09-03 2007-09-03 ! I DB61IT 408-2007 I ! J *:fJFffE r:p ft:iRAjJ *411 Ilft:iR. 1 1 *ffEr:p$AOOMiliAfJFffE ttixtt;ffl 1 I f DB61tr 408-2007 ;js:*ffiftE1m T 1t;:ylJE4JgJ9 D-Lft/L LmilJ:wIr7Jt;:o * 1 ftE 13: J+ T 4W)D-L/LLftIJWlJE 0 2 ;m5iHiijIm3i:f!f r )lUX11: 9 BJ J

2、Eli1;js:j1lIJiIJ+j TID JJX;) *fitEJo fL1id1 E3 ,1tIj tEJ ij IJ+j X 11:, :It)fiJFi FnTA1 .(Wm3).UJ+T;js:m,TID,.;js:ftEtEJ liki!fi5J1!fJij:l!%J;:x1Hn.ii:;js:o 1;li!:;I E3 Jt IJ IJ+ X14, ;1=tiiWr*13:Jtl r;js:*ffiftE 0 EN-ISO 8069 3)J.ltWJ9?LEcLft*ttA1 EN 12631 *:fIIJfidt9D-Lmi;fILL () IJJE DIN 10335 4W

3、J:f114WJ1ttlM9D-/L-LM1JE D-LDH L-LDH NAD NADH GPT U A C D-LftJltI.!W L-LJlUW MIJ:,npt-=1rM j3 -1W-n-ft1TM :fr p;jill0$ 1 11 rno1 IJ 4io J.at 0 ,mN! tr i: p;Jill (pyruvate), L-!Lfffitf L-!JiLJlttl,f CL-LDH) fffta1;tflllt1to JiJIV.5:tJ,): H.OII (0 D-Lactate + NAD pyruvate + NADH + II (2) L-Lactate + N

4、AD LUlll pyruvaet + NADH + H Jfj$iij CGPT) H1HtRj:q1ijlIW (pyruvae), fIjTjAJJiJW (pyruvae) ;fll NADII I.J1:.JJX;, JiJ.if.:rJJ; DB61/T 408-2007 GPT (3) Pyruvate + L-glutarnate L-alanine + 2oxoglutarate 1?J.1:B(.p%Jix;iJ NADH D-Lactate. L-Lactate iJW7t!ijIJfftE1.titit)to 1:fiX:f1 NADH iJn)l 7tJ.l1Ifil

5、J:f 340nm rIJ:m:o 5. 1 )(J itJ:B;t 5.2 NAD 5. 3 GPT 5.4 DLDH 5. 5 LLDH 5. 6 HCl 5. 7 NaOH 5.8 KOH _ fl.J 5.9 li :K 5.10 illPJ1!tJt 6. 1 f$lHE: (5 mL. 1 mL 50200 l.l L 1O50 Jl L) 6.2 btellIl: 7tf5:1.0cm 6.3 3J5t7t7tJ.lit, TlJ3k:(K, fr:HOnmW 0 6. 4 1JT1, JLH 10 Jl m 0 6.5 6.6 tBjll1ilkm 6. 7 71nJEiia

6、6. 8 lfJl 6.9 ,m,AA.B.ft,4,.W 7. 1 lIt1f I Jj!jl*U 7.1. 1 1tjH.t; flFlfX4. 75gXXitJ;t,f110. 88R;f1L-fiJ:lW.ML, 110A60 mUIH1*, J7tmlt-Jfl, )fj2 mol/L NaOH V pH1O.0; 7.1.2 NAD I1i; 1!t+l B1, ImL JI7JJ.fM35mg=f*) liP JJNADT 1fo 7. 1. 3 GPT Hi ffj fit, +lO. 7mLm:,$)Km:lf.ll00UiJGPT=f;fJJlmJJGPT;,J1o 7.1

7、.4 D-LDH ;gHi 1JtJ fIt. JtJ O. 7mL1M:t4)KmM A13800JIf.lD-LDI-Ic 7.1.5 L-LDH igHi f!t,LB 111 , ,LBO. 7mL:lli:*mffFL.k,j13800UiJD-LDHo 7.1.6 He): 2 moIlL 7.1.7 NaOH: 2moi/L 2 DB61n 408-2007 7.1.8 KOH: 2mollL 1. 2 litffiIJ1i !fX.2mL (g) 4tJj, noA98mUlUJ./, mrl1ifEth/HlOmin, 1t5t1, ii, 7J!J.jr-:ftEtWlIJ

8、o 1. 3 #tWIlj (jJt 1 ilHT) 1.3. 1 i9:s-t!m: !fX.btllJl, f-t;k , 1. 000 mL J./, itJ!i 5min J-;!fX.D .j,C 7. 3. 2 l+1:n:p !fX.btlIll., f-k7, 0.100 mL1J.t?, 4/j( O. 900 mL lft-frJl5 min Fn;!fX.ll,nit (:kJ 30 min), -;!fX.D 1 #l;9!lj l 5t)JIJ1E3:8 L.jf4:*IJD,1U0A (1) : f:,AIJ-il,= A2-AI J IH-A,-A: UI . ,

9、 . . .(1) :5J. IJ 1tl 1E:i: AJJH*rrIl1 fIJ0A (2) : f:,AL-t!W= A,-A 2 J II-A,-A, ,11 .(2) 8 it. itHI 0 J:.(3)itm: tR 2. 260X 90. 1 C X L:1AI.1UIt X f X 1. 00 X O. 100 X 1000 2. 036 CV-MIt (g/UrfldR1ff) -X 100( g / 100g) .(4) C;IUIf (g/Uflldffilf) G.-liAr = - X 100( g / 100g) . . . .(5) c WluW,9fIJ#!.

10、!r (giL) MW l:9lljWJPt IJ ffjJ( !ViiiL L (jJmw 90. 19!mol) Illi:UlLU: (jJ(!Viii 89.1g/mol) VI ctJl4JlljlitfHIJ/f;,1*fJ(, 1f7: mL *IJm.IJ1*, . f f nh m.I1Jiff: itt d 5JlIJJtW, Ir. em NADPH IJWl7t:rz: ft 340 run =6.3 L mmor-1emI L1A rJtHi-1-*ff-/l!f.J D-LIL L*ifi 9.2 MitS:?:!f.Jmm 9.2. 1 ;1JnMitS:?:j;

11、fiflj,W, tu O.050ml lJ!VirRt11QfIJfftJlUl:B-frm , (Ar)#rt:#F 4 DB611T 408-2007 :tJnAt;fft:p lJm A ffMl f1:l rm-nn+; - - xx i=tn;!dlltP NAD.f 1f. GPT; fffi1r.tm D-LJW:t:lmEixL1LJW:t:lm m;J 1.000mL 0.200mL O.020mL - 1.000mL 1.000mL O.200mL O.020mL O. lOOmL - O.900mL 1. OOOmL 0.200mL O.020mL - O. 100mL

12、 O.900mL 1. OOOmL 0.200mL 0.020mL O.050mL O.050mL 0.900mL 1liil5 iH-rr JR:tttrl/J 11I AI , JRt 7. 3;ff.z2t til ;ff.zlar - m1ta ) X 100 (%) . (6) 5 DB61ff 4082007 2, btjflj.p, !IIG1tIJYJIJtjl, JiIjTY.ff.r 2C8Co 1tuf.iX;1J NAD :ftfr 2C8CWJ SE=jii, If-15C-25Cf4 t-iiJ1*fJWi1- fj a 1tuf.iX;FlJ D-MDH . Ltfft 2C8CPJJE 5 7(0 F1fc 9, D-Ui*ILLmiiJfij(!Ul1Y:Jv.7t!bHt-m:. 6