GOST 32711-2014 Fruit and vegetable products Determination of sulfur dioxide by Enzymatic method《水果和蔬菜产品 采用酶法测定二氧化硫》.pdf

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1、 , () INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION (ISC) 32711 2014 2014 327112014 II , - 1.092 . 1.22009 . -, . , , , 1 () - ( -) 2 3 , ( 25 2014 . 45) : ( 3166) 00497 ( 3166) 00497 AM KZ KG RU TJ UZ UA 4 1 2014 . 852- 327112014 - 1 2016 . 5 NMKL-135:1990 - ( ) ( ) NMKL-135:1

2、990 Sulphite. Enzymatic determination in foods (Nordic Committee on Food Analysis NMKL) 6 54894 2012 7 - , . () - - . , , 2014 -, - 327112014 1 Fruit and vegetable products. Determination of sulfur dioxide by Enzymatic method 2016 01 01 1 : , , , - , , , , - ( ), -, , . . (), , , , - - . ( ) 10 500

3、/3 (1). 3 /3 (1).2 - : 36962013 . -1 5725-12003 ( ) -. 1. 5725-22003 ( ) -. 2. 5725-62003 ( ) -. 6. / 170252009 - OIML R 76-12011 . - . 1. . 12.1.00588 . - 12.1.00776 . . - 12.1.01893 . - - . 12.1.01979 . -. - 2 19577 . . 1 52501-2005 . . 2 12.1.0192009 - . . . 327112014 2 177074 ( 104283, 478880) .

4、 -, , , . 376978 . . 420179 . . 432877 . . 1065273 . -N,N,N,N- , 2- ( ). 1202676 . 2533682 . , 25794.183 . - 2631384 . , 2667185 , -. 2831189 . - 2849890 . . 2922791 ( 835-181) . . 1. 316432012 . - - , 1 , - . (), - () . , , , , . 3 , 3.1 -: 3.1.1 : , . 3.1.2 : (SO2) - SO2.3.2 : 3.2.1 ( ) , - () 110

5、6 25 .3.2.2 (NAD) , . 3.2.3 (NADH) , . 3.2.4 - (NADH-POD) . 3.2.5 () , . 3.2.6 (SO2-OD) - , . 4 : - (SO2-OD) : SO3 + 2 + 2 SO2-OD SO4 + 22 327112014 3 - (NADH-POD) - (NADH): 22 + NADH + + NADH-POD 22 + NAD+ AH, , - : 334, 340 365 . 5 , , , 5.1 , 340 , , , 334 365 , - 1 %. 5.2 5 20 2 10 3.5.3 4328, .

6、 . . 5.4 4201, NaHCO3, . . . 5.5 , Na2-, 95 %. 5.6 - (NADH-POD), 1 , CAS 9032-24-0. 5.7 (SO2-OD), 1 , CAS* 9029-38-3.5.8 (), 20 , CAS* 9029-44-1. 5.9 195, . . . 5.10 3769, (NH4)2SO4, . . . 5.11 -N,N,N,N- , (Na2-, ) 10652, . . . 5.12 , 98 %. 5.13 *, : 1 30 3 8,0; 2 30 0,4 ; 3 0,3 3 - (NADH-POD) 3 ; 4

7、 1,6 3 (SO2-OD) 2,5 .5.14 - 2 3, 0,05 . .5.15 (1-) OIML R 76-1, - 0,01 . 5.16 (), 3000 5000 1, , . . .5.17 1-2-1-1, 1-2-1-2, 1-2-1-5, 1-2-1-10 1-2-1-25 29227 28311 , - 1 %. 5.18 2- 1770: 1-50-2 1-1000-2; 2-50-2, 2-100-2 2-1000-2; 1-10-0,1, 1-15-0,1 1-20-0,1. 5.19 25336: ; CAS (Chemical Abstracts Ser

8、vice - CAS). * R-Biopharm AG. , (. 7.2). 327112014 4 -1-100 -1-1000.5.20 0,20 0,45 13 47 . 5.21 , 4000 1 - . 5.22 400 1200 1.5.23 20 1 3.5.24 12026. 5.25 3696, 2- . 5.26 2 5 - . 5.27 15 50 3 - . 5.28 . 5.29 28498, 0 100 , 1 .5.30 . , , - , , - , . 6 26313, 26671. 6.1 6.1.1 31643 ( 6.2). (0,15 0,05) - 2 3 , 4000 1 - 10 0,45 . - . 6.1.2 100 /3 . 1520 3 2 3 - (5,5 0,5) . , -,