DB45 T 1951-2019 水牛胚胎冷冻保存技术操作规程.pdf

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1、g44g38g54g3 g25g24g17g19g21g19g17g22g19g3 g37g3g23g22g3 g3g3g3g3g3g3 DB45 g5301g16309g3876g7173g14368g8945g2416g3430g7151g7741g2044g3 g39g37g3g23g24g18g55g3g20g28g24g20g258g21g19g20g28g3 g3g3g3g3g3g3 g8810g10385g14096g14084g2029g2033g1555g4494g6326g7525g6915g1426g16378g12353g3 g48g68g81g76g83g88g79g

2、68g87g76g82g81g3g85g72g74g88g79g68g87g76g82g81g3g73g82g85g3g70g85g92g82g83g85g72g86g72g85g89g68g87g76g82g81g3g82g73g3g72g80g69g85g92g82g86g3g76g81g3g69g88g73g73g68g79g82g3 g3 g3 g21g19g20g28g3g16g3g19g24g3g16g3g22g20g2567g5177g3 g21g19g20g28g3g16g3g19g25g3g16g3g22g19g4564g7155 g5301g16309g3876g7173g

3、14368g8945g2416g5176g3440g11527g11673g12759g10812g4726g3 g3 g3 g2567g5177 g3 g39g37g23g24g18g55g3g20g28g24g20g178g21g19g20g28g3 g44g3 g11556g3 g8535g3 g2173g16432g3g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g

4、17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g3g44g44 g20g3g14643g3364g3g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g

5、17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g3g20 g21g3g16372g14643g5719g5445g11096g7095g1318g3g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g

6、17g17g17g17g17g17g17g3g20 g22g3g7519g16925g2748g4554g1145g3g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g3g20 g23g3g16901g2162g45

7、1g13895g7552g451g16878g3895g2748g4558g20668g4564g16305g8818g3g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g3g21 g24g3g2023g2027g14090g14078g11444g17977g6425g2748g7735g16864g3g17g17g17

8、g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g3g21 g25g3g2023g2027g17837g1411g11444g2038g3895g3g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g

9、17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g3g22 g26g3g14090g14078g11444g2023g2027g3g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g1

10、7g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g3g22 g27g3g17246g4488g1086g17920g17859g3g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17

11、g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g3g23 g28g3g14090g14078g11444g16403g2027g3g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17

12、g17g3g23 g19572g5509g36g708g16372g14643g5719g19572g5509g709g3g16901g2162g451g13895g7552g2748g16878g3895g3g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g3g25 g19572g5509g37g708g16372g14643g5719g19572g550

13、9g709g3g14090g14078g2023g2027g2748g16403g2027g9186g1411g3g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g3g27 g19572g5509g38g708g16372g14643g5719g19572g5509g709g3g14090g14078g2023g2027g2748g16403g2027g16864g55

14、09g16024g3g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g17g3g28 g39g37g23g24g18g55g3g20g28g24g20g178g21g19g20g28g3 g44g44g3 g2179g3 g16438g3 g7516g7735g2038g6457g10135g42g37g18g55g3g20g17g20g252g21g19g19g28g13577g209

15、0g11444g16372g2121g17319g14713g452g3 g7516g7735g2038g11105g5295g16303g3870g7167g14362g8939g2410g1996g1098g1996g7553g2485g6656g2090g452g3 g7516g7735g2038g11105g5295g16303g11148g10391g1098g7735g2038g2374g6320g7519g4100g2696g1354g5506g2579g452g3 g7516g7735g2038g17319g14713g2437g1405g726g5295g16303g3870

16、g7167g14362g8939g2410g8804g10379g11844g12454g6256g452g3 g7516g7735g2038g1131g16305g17319g14713g1258g726g7576g7253g14499g451g4682g8847g2430g451g18177g9127g14625g451g21748g2256g12181g451g16307g5295g14092g451g21748g14556g20425g451g1214g1996g9207g451g7857g17236g4145g452g3 g39g37g23g24g18g55g3g20g28g24g2

17、0g178g21g19g20g28g3 g20g3 g8810g10385g14096g14084g2029g2033g1555g4494g6326g7525g6915g1426g16378g12353g3 g20g3 g14649g3370g3 g7516g7735g2038g16372g4554g1206g8804g10379g14090g14078g17333g1406g9305g2023g2027g6320g7519g16372g12347g712g2357g6428g12347g5311g2374g2023g2027g2748g10731g10931g2374g2023g2027g6

18、320g7519g16305g8818g452g3 g7516g7735g2038g17970g11096g1214g1411g1973g14090g14078g2748g17994g17911g1411g3910g2567g13038g451g2437g13038g4480g14094g17240g1973g7278g5598g2567g13038g451g1411g13558g14094g7784g12331g7997g6320g7519g11087g1239g11444g14090g14078g452g3 g21g3 g16378g14649g5725g5451g11102g7101g1

19、324g3 g1083g2119g7095g1318g4649g1214g7516g7095g1318g11444g5316g11096g7263g5621g1085g2591g4673g11444g452g2065g7263g8984g7189g7503g11444g5445g11096g7095g1318g712g1269g6256g8984g7189g7503g11444g10360g7516g17970g11096g1214g7516g7095 g1318g452g2065g7263g1085g8984g7189g7503g11444g5445g11096g7095g1318g712g

20、1958g7472g7136g10360g7516g708g2357g6428g6256g7481g11444g1566g7017g2437g709g17970g11096g1214g7516g7095g1318g452g3 g42g37g18g55g3g24g23g24g27g3g3g9186g8798g11087g10393g4585g3224g3 g42g37g18g55g3g21g24g27g27g20g3g3g10379g14090g14078g3 g22g3 g7525g16931g2754g4560g1151g3 g1083g2119g7519g16925g2748g4554g1

21、145g17970g11096g1214g7516g7095g1318g452g3g3 g22g17g20g3 g3 g17339g1412g9311g1555g4494g3g3g70g85g92g82g83g85g72g86g72g85g89g68g87g76g82g81g3 g4662g13567g2023g2027g1549g6356g2162g3892g10806g11444g14090g14078g7022g13726g3416g713g20g28g25 g3 g263g708g9186g8798g709g11444g17333g1406g9305g5334g1117g712g145

22、5g1958g7136g19576g1299g16978g7346g7206g1676g8594g712g1278g13884 g17931g15996g19375g7503g1549g4488g452g3g3 g22g17g21g3 g3 g1417g1979g14096g14084g3g3g76g81g3g89g76g89g82g3g72g80g69g85g92g82g86g3 g17994g17911g9712g13136g1523g17931g8701g11148g17333g7072g6594g2469g712g1981g18423g11096g14362g10086g1236g18

23、301g6214g1258g5141g6584g13038g712g1278g8701g11148g11444g17859g2469g12753g6214g4480g4571g16386g1973g3342g7014g2561g14050g2144 g1072g4554g7206g7503g11444g7193g7503g14090g14078g452g3g3 g22g17g22g3 g3 g8810g10385g14096g14084g1417g3916g11093g1245g6326g7525g3g3g76g81g3g89g76g87g85g82g3g83g85g82g71g88g70g8

24、7g76g82g81g15g44g57g51g3 g8273g6415g8804g10379g1411g1973g10719g3763g712g3416g1411g3910g1258g5141g6615g2150g7569g1318g1083g712g17994g17911g18423g19702g8804g10379g2469g5138g1082g7514g6208g10191g11444g2469g8701g13558g14094g13567g1411g3910g6208g10191g3625 g1963g451g13038g4480g1411g3910g14823g14125g451g1

25、3038g2469g1411g3910g2567g13038g2748g2567g13038g2469g14090g14078g1411g3910g3625g1963g12665g6320g7519g11087g1239g14090g14078g452g3g3 g22g17g23g3 g3 g1417g3916g2573g13044g3g3g76g81g3g89g76g87g85g82g3g73g72g85g87g76g79g76g93g68g87g76g82g81g15g44g57g41g3 g8273g6415g1411g1973g10719g3763g1455g2858g1187g226

26、4g10393g11444g13038g4480g2748g2469g4480g3416g1411g3910g1258g5141g6615g2150g11444g10719g3763g1117g4540g6208g2567g13038g17911g12347g11444g6320g7519g452g3g3 g3 g3 g3 g39g37g23g24g18g55g3g20g28g24g20g178g21g19g20g28g3 g21g3 g22g17g24g3 g3 g14100g9084g1979g2443g13044g4486g8990g4666g6326g7525g3g3g76g81g87

27、g85g68g70g92g87g82g83g79g68g86g80g76g70g3g86g83g72g85g80g3g76g81g77g72g70g87g76g82g81g15g44g38g54g44g3 g1615g2265g7278g5598g6909g1420g13099g13583g4662g2437g1072g13038g4480g8984g4660g1941g2469g4480g1973g1455g1958g2567g13038g11444g6320g7519g452g3g3 g22g17g25g3 g3 g1417g13564g14100g7790g12337g8003g3g3g

28、86g82g80g68g87g76g70g3g70g72g79g79g3g81g88g70g79g72g68g85g3g87g85g68g81g86g73g72g85g15g54g38g49g55g3 g4662g1072g1114g2264g10393g11444g1411g13558g14094g7784g12331g1941g2539g7784g11444g6208g10191g2469g8701g13558g14094g1117g712g1239g11087g1086g1483g1411g2264g10393g18055g1360g6208g1325g3626g7516g1072g14

29、372g11444g2264g10393g2622g1299 g6320g7519g452g3 g23g3 g16907g2168g573g13901g7558g573g16884g3901g2754g4564g20674g4570g16311g8824g3 g23g17g20g3 g16907g2168g3 g6256g11096g16901g2162g451g14751g2801g1301g2554g16305g8818g16369g19572g5509g36g17g20g452g3 g23g17g21g3 g13901g7558g3 g6256g11096g13895g7552g1636

30、9g19572g5509g36g17g21g452g3 g23g17g22g3 g16884g3901g3 g6256g11096g1131g16305g16878g3895g16369g19572g5509g36g17g22g452g3 g23g17g23g3 g4564g20674g4570g16311g8824g3 g5141g1420g2410g5316g16878g13726g2038g3895g4564g451g13635g2018g19492g451g7184g14844g19492g451g13558g14094g1549g4488g4564g12665g452g1958g11

31、17g712g13635g2018g19492g19858g12319g1085g5575g4671g1214g22 g3 g80 g21 g712g5316g4537g13726 g7460g16019g7692g2748g13147g3910g9887g712g13147g3910g9887g5482g5334g1085g5575g1406g1214g20g17g24 g3 g58g18g80 g22 g452g13147g3910g9887g12753g17421g3424g19858g1085g5316g17333g17911g21g17g24 g3 g80g712g8703g8529

32、g10135g4660g7206g19492g1130g21g19 g3 g80g76g81g794g22g19 g3 g80g76g81g452g7184g14844g4564g11444g7184g14844g12665g13527g7472g1406g7735g2038g5316g17902g2144g1079g13527g712g6909g1420g2410g5316g17902g2144g11438g13527g452g3 g24g3 g2029g2033g14096g14084g11450g17983g6431g2754g7741g16870g3 g24g17g20g3 g2618

33、g14096g14084g2567g14056g19564g8683g2754g17246g18437g12671g13533g11450g7741g16870g3 g24g17g20g17g20g3 g14090g14078g2561g14050g19558g8677g2114g2102g2748g14090g14078g2102g13527g16884g4554g5316g12630g2616g42g37g18g55g3g21g24g27g27g20g11444g16372g4554g452g8804g10379g14090g14078g11444g2561g14050g7206g1949

34、2g11560g17843g1214g10379g5316 g6656g2173g20g21g3g75g452g14090g14078g2023g2027g2173g11444g17240g18431g5316g17902g2144g37g13527g708g2651g37g13527g709g1301g1082g452g3 g24g17g20g17g21g3 g7809g8041g14090g708g48g47g709g451g14372g4598g7809g8041g14090g708g38g48g709g451g7193g7503g3322g14090g708g40g37g709g451

35、g3322g14090g708g37g47g709g451g6297g5456g3322g14090g708g40g59g37g709g451g4517g2374g3322 g14090g11g43g37g12g11444g13657g1993g5316g13583g1072g16372g14643g452g3 g24g17g21g3 g1091g2626g12291g12977g14096g14084g11450g7741g16870g3 g1411g1973g14090g14078g7735g16864g1130g44g57g40g712g1411g3910g2567g13038g1409

36、0g7735g16864g1130g44g57g41g712g14094g17240g1973g2437g13038g4480g7278g5598g8984g4660g14090g14078g7735g16864g1130g44g38g54g44g712g1411g13558g14094g7784g12331 g7997g14090g14078g7735g16864g1130g54g38g49g55g452g3 g24g17g22g3 g14096g14084g13564g12759g7741g16870g3 g24g17g22g17g20g3 g13558g12753g1082g7735g1

37、6864g11444g14090g14078g1553g5791g2651g2023g2027g7206g19492g451g14090g14078g12285g12971g451g14090g14078g1114g7072g451g2561g14050g19558g8677g451g17240g18431g452g1959g1411g7735g16864g16369g4018g1083 g12138g1467g726g4018g21g19g20g26g5284g26g7480g21g19g7189g2023g2027g21g7626g36g13527g44g57g41g6297g5456g3

38、322g14090g712g1958g11435g16864g11444g5311g2599g1130g20g19g20g712g2121g7735g16864g1130g20g26g19g26g21g19g3g44g57g41g3g21g40g59g37g3 g39g37g23g24g18g55g3g20g28g24g20g178g21g19g20g28g3 g22g3 g36g3g20g19g20g452g13884g4018g7628g7263g5507g3929g2023g2027g1108g7626g36g13527g1411g13558g14094g7784g12331g7997g

39、6297g5456g14090g14078g712g1958g13558g14094g1483g1411g10379g11444g5311g2599g1130g23g19g19g20g712g2121g7735g16864g1130 g20g26g19g26g21g19g3g54g38g49g55g3g21g40g59g37g3g36g3g23g19g19g20g452g3 g24g17g22g17g21g3 g2620g7206g1674g4013g16918g4717g11444g14090g14078g2023g2027g16024g7788g712g8984g7230g14090g14

40、078g6256g7481g11444g11560g1955g1553g5791g712g14090g14078g1553g5791g11435g16864g16024g16369g19572g5509g38g452g3 g25g3 g2029g2033g17843g1417g11450g2044g3901g3 g25g17g20g3 g12353g5317g2380g13564g12759g11450g2044g3901g3 g12347g5311g2374g2023g2027g13558g12753g7552g7113g5316g7184g8706g451g13888g1406g9305g

41、712g13558g12753g16372g7788g1301g19g17g21g24 g3 g80g47g1130g4556g712g13558g12753g3910g16370g8595g5224g451g7184g2568g5522g451g7184g16114g11269g712 g4657g2579g1109g4598g452g3 g25g17g21g3 g10737g10937g2380g2029g2033g17843g1417g11450g2044g3901g3 g2591g1301g18423g11096g2934g1098g2374g11444g10731g10931g237

42、4g2023g2027g17837g1411g727g1167g2591g1301g18423g11096g19g17g21g24 g3 g80g47g13558g12753g712g11096g2096g10359g4662g1958g1072g12575g7116g2103g712g5522g6208g1072g6234g7116 g2103g19858g712g1301g8596g2103g19858g1420g1130g10731g10931g2374g2023g2027g11444g17837g1411g712g4018g3374g20g6256g12138g452g3 g3 g33

43、80g20g3 g10737g10937g2380g2029g2033g17843g1417g3 g26g3 g14096g14084g11450g2029g2033g3 g26g17g20g3 g14096g14084g11450g12353g5317g2380g2029g2033g3 g26g17g20g17g20g3 g14096g14084g11450g9037g9178g3 g4662g36g13527g451g37g13527g14090g14078g13726g1214g14090g14078g3625g1963g9186g1117g9031g9172g21g794g22g852

44、9g452g3 g26g17g20g17g21g3 g14096g14084g11450g5289g16023g3 g12347g5311g2374g14090g14078g2023g2027g9186g6208g2102g16369g19572g5509g37g452g4662g9031g9172g2622g11444g14090g14078g7022g1941g2023g2027g9186g1117g712g4564g9305g1083g5283g16017g20g24 g3 g80g76g81g794g21g19 g3 g80g76g81g452g3 g26g17g20g17g22g3

45、g14096g14084g11450g16123g12759g3 g4662g5283g16017g2622g11444g14090g14078g16117g1941g19g17g21g24 g3 g80g47g13558g12753g1117g712g16117g12753g20138g5311g1130g726g2023g2027g9186g708g21 g3 g70g80g794g22 g3 g70g80g709g279g8772g8977g708g19g17g21 g3 g70g80g794g19g17g24 g3 g70g80g709 g279g2023g2027g9186g2256

46、g14090g14078g708g25 g3 g70g80g794g26 g3 g70g80g709g279g8772g8977g708g19g17g21 g3 g70g80g794g19g17g24 g3 g70g80g709g279g2023g2027g9186g708g21 g3 g70g80g794g22 g3 g70g80g709g712g1981g11096g4657g2579g12985g8942g8804g4657 g2579g452g8703g7007g13558g12753g2591g16117g20g794g21g7626g2561g14050g10470g2021g10

47、86g1483g1411g11560g2620g11444g14090g14078g712g4018g3374g21g6256g12138g452g3 g3 g3380g21g3 g14096g14084g11450g16123g12759g20144g5317g3 g3 g3 g3 g39g37g23g24g18g55g3g20g28g24g20g178g21g19g20g28g3 g23g3 g26g17g20g17g23g3 g2029g2033g12353g5317g3 g4662g16117g4013g14090g14078g11444g2023g2027g13558g12753g1

48、6117g1941g2256g4013g9186g8798g11444g12347g5311g2374g19581g9305g1306g1973g712g7929g3726g1072g3940g7501g1082g6214g13877g7501g3910g712g1301g23 g3 g263g18g80g76g81g11444g17999 g10679g19581g14371g16g26 g3 g263g712g11096g19322g4480g13648g4013g9885g14844g7929g712g9128g1941g9186g8798g712g17909g17999g6613g16

49、406g13558g12753g7929g3726g2748g2023g2027g9186g11444g1236g11132g19858g17931g15996g7997g2016g452g7997g2016g2622 g3416g16g26 g3 g263g5283g16017g24 g3 g80g76g81g712g1301g19g17g22 g3 g263g18g80g76g81g11444g19581g9305g17999g10679g19581g14371g16g22g25 g3 g263g2622g712g11556g6613g4662g13558g12753g6341g1941g

50、9186g8798g1973g452g3 g26g17g21g3 g14096g14084g11450g10737g10937g2380g2029g2033g3 g26g17g21g17g20g3 g9192g8804g11450g2044g3901g3 g2566g1072g1549g9305g3878g712g4662g9186g8798g2256g14371g13526g1081g2102g1147g1212g3892g712g11526g4013g2622g3895g11096g452g3 g26g17g21g17g21g3 g2029g2033g5289g16023g9192g275