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    KS I ISO 9408-2006 Water quality-Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium by determination of oxygen demand in a closed respirometer《水.pdf

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    KS I ISO 9408-2006 Water quality-Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium by determination of oxygen demand in a closed respirometer《水.pdf

    1、 KSKSKSKS SKSKSKS KSKSKS SKSKS KSKS SKS KS 2006 9 29 http:/www.kats.go.krKS I ISO 9408 KS I ISO 9408: 2006 (2011 )I ISO 9408: 2006 : ( ) ( ) KIST ( ) : (http:/www.standard.go.kr) : :2001 1 10 :2006 9 29 :2011 12 26 2011-0622 : : ( 02- 509-7270) (http:/www.kats.go.kr). 10 5 , . ICS 13.060.70 KS I ISO

    2、 9408: 2006(2011 ) Water quality Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium by determination of oxygen demand in a closed respirometer 1999 2 ISO 9408 Water quality Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium by d

    3、etermination of oxygen demand in a closed respirometer . 1. (respirometer) . . a) . b) (KS M ISO 10634 ). c) CO2 . d) (respirometer) ( ) , . e) . 7.3 (KS M ISO 8192 ). . KS M ISO 15462 . 2. . 2.1 (ultimate aerobic biodegradation) , (mineralization) (mineral salts), (biomass) . 2.2 (primary biodegrad

    4、ation) ( ) . 2.3 (activated sludge) (biomass) (bacteria) . 2.4 (concentration of suspended solids of an a ctivated sludge) 105 I ISO 9408: 2006 2 . 2.5 (biochemical oxygen demand, BOD) mg /mg( g) . 2.6 (chemical oxygen demand, COD) , (O2 mg/L) . mg /mg( g) . 2.7 (theoretical oxygen demand, ThOD) , .

    5、 mg /mg ( g) . 2.8 (dissolved organic carbon, DOC) (相分離 ) 40 000 m s 2 15 0.2 m 0.45 m . 2.9 (lag phase) , 10 % . (days) . 2.10 (maximum level of biodegradation) . % . 2.11 (biodegradation phase) (lag phase) 90 % (days) . 2.12 (plateau phase) (days) . 2.13 (pre -exposure) (pre-incubation) , . 2.14 (

    6、preconditioning) , (pre-incubation) , . 3. (static aqueous test system) . . 100 mg/L (ThOD) 100 mg/L , . (respirometer) , ( ) . I ISO 9408: 2006 3 28 , . (ThOD) (COD) . (DOC) , . . 4. (test environment) , 2025 (1 ) . 5. (reagents) . 5.1 DOC 1 mg/L 5.2 5.2.1 (composition) 5.2.1.1 A (5.1) KH2PO48.5 g,

    7、 K2HPO421.75 g, Na2HPO4 2H2O 33.4 g, NH4Cl 0.5 g 1 000 mL . pH 7.4 . 5.2.1.2 B 5.1 7 (MgSO4 H2O) 22.5 g 1 000 mL . 5.2.1.3 C 5.1 2 (CaCl2 2H2O) 36.4 g 1 000 mL . 5.2.1.4 D 5.1 6 (FeCl3 6H2O) 0.25 g 1 000 mL . (HCl) . 5.2.2 5.1 800 mL A 10 mL B D 1 mL 1 000 mL . 5.1 1 000 mL . . A , B , C 6 . 5.3 (ca

    8、rbon dioxide absorber) 10 mol/L (potassium hydroxide) (soda lime pellets) . 5.4 5.1 100 mL HgCl2 1 g . 5.5 5.1 NaOH 0.1 mol/L 0.5 mol/L . 5.6 5.1 (HCl) , 0.1 mol/L 0.5 mol/L . 6. . 6.1 (respirometer) (respirometer) D . (respirometer) . (respirometer) (water-bath) . . . 6.2 (water -bath) (4. ) 6.3 (D

    9、OC) DOC ( ). I ISO 9408: 2006 4 6.4 COD ( ) 6.5 4 000 rpm . (membrane filter; 0.2 m 0.45 m ) . 6.6 pH ( ) 7. 7.1 7.1.1 5.2 , 100 mg/L , ThOD 100 mg/L . ( , ) . . . COD KS M ISO 6060 . KS M ISO 10634 . 7.1.2 (reference compound) , 60 % (aniline) (sodium benzoate) . 100 mg( )/L( ) , 7.1.1 5.2 . 7.1.3

    10、(check inhibition) ( , ), 5.2 7.1.1 7.1.2 100 mg/L . 7.2 7.2.1 7.2.2 7.2.4 . 7.1.2 9. . BOD . . . . ( , = x %, .) . , Zahn Wellens (KS M ISO 9888) SCAS (KS M 9138) ( , ) . . 9. (%) 103106/mL SS 30 mg/L 10 % I ISO 9408: 2006 5 1 000 mL 110 mL . 7.2.2 . ( KS M ISO 11923 ). , 7.2.1 . (5.2) . , . 7.2.1

    11、(SS) 30 mg/L . 7.2.3 . . , . 1 . 7.2.4 . . . . 7.3 6.1 D (respirometer) . . 7.1.1 (FT)( 2 ) 2 (FB) 7.1.2 1 . Fc . 7.1.3 1 ( FI) 7.1.1 , 1 (FS) , 2 (5.4) 2 . 1 (5.2), (7.2), (7.1.1) (7.1.2) . 5.3 . pH , 5.5 5.6 pH 7.4 . (6.2) , 4. , . ( ) BOD( ) (respirometer) . (respirometer) . ( ) , . 28 . , 1 2 .

    12、pH . DOC (t = 0) (t = t) . (t = 0) . 15 4 000 rpm (6.5 ). DOC 4 , 48 . DOC I ISO 9408: 2006 6 , (striping) . . ( t) FT FS . 1 (5.2) (7.1.1) (7.1.2) (7.2) FT FT FB FB FC FI ( ) FS ( ) , . spot , spot . ( B ). 8. 8.1 8.1.1 (specific) BOD BOD . (1) BOD(Bs) . (7.3 B ). BS=TCBtt (1) BS: (specific) BODmg(

    13、O2)/g( ) Bt: t FT BOD(mg/L) BBt: t FB BOD(mg/L) TC: (g/L) 8.1.2 BOD (ThOD, A ) COD . (2) (3) . DThOD =ThODS100 (2) DCOD =CODS100 (3) DThOD= t ThOD DCOD = t COD BS = mg(O2)/g( ) ThOD = mg(O2)/g( ) I ISO 9408: 2006 7 COD = mg(O2)/g( ) COD ThOD COD ThOD . . 8.1.3 DOC DOC (4) Dc FT . DC=cT0cT0cBcT1tt100

    14、 (4) cT0: DOC mg/L, t = 0, FT cB0: DOC mg/L, t = 0, FB cTt: DOC mg/L, t = t, FT cBt: DOC mg/L, t = t, FB cT0 cB0 . 8.1.4 (5) Fs DS . DS=STS 100 T: mg/L, FT, t = t S: mg/L, FS, t = t 8.1.5 , FC, FS FI DOC . 8.2 DThOD DCOD BOD . , . , (lag phase) (degradation phase) . FT 20 % , ( C ). FC FS FI . ( , ), “ ” . . FI 25 % FT , . . FS ( ) BOD (10 % ) , . DOC / pH . 9. I ISO 9408: 2006 8 9.1 . a) FC 14 60 % . b) FB BOD 28 60 mg/L , 20 mg/L 30 mg/L . a) b) , . 9.2 FI , FI 40 % . . 9.3 pH pH 6 8.5 ( , ) 60 % , . 10. . a) b) c) , ( ) d) ThOD COD e) f) , , g) (respirometer) h) i) DOC j) FS


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