KS I ISO 23893-1-2009 Water quality－Biochemical and physiological measurements on fish－Part 1：Sampling of fish handling and preservation of samples《水质 鱼的生物化学和生理测量 第1部分 鱼的取样、处理和试样的保.pdf

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2、 238931:2009 1： , Water qualityBiochemical and physiological measurements on fishPart 1：Sampling of fish, handling and preservation of samples 2007 1 ISO 238931, Water qualityBiochemical and physiological measurements on fishPart 1：Sampling of fish, handling and preservation of samples , . 1 , , . (

3、biomarker) . . - , 2 , , 1 , () . , , , , . , (anthropogenic compound) . . , , ( ) . , , , . (subcellular) . KS I ISO 238931:2009 2 . , , ( ) . , . . ( ) , . (, , , ) , . . , ( .), . . (Perca fluviatilis) (viviparous blenny, Zoarces viviparous) . . . . 3 3.1 3.1.1 , . 3.1.2 3.1.3 , . 3.1.4 , , , . 3

4、.1.5 , , . 3.1.6 (GPS), . 3.1.7 , . 3.1.8 , . 3.1.9 , , . 3.1.10 ( , , pH , ), . KS I ISO 238931:2009 3 3.1.11 , (echo-sounder) . 3.2 3.2.1 , , 100 m . 3.2.2 , . 3.2.3 , , 3.2.4 , . 3.2.5 , ( 4.4.3 ). 3.2.6 , , , , , 3.2.7 , . 3.2.8 , (, , ) . 3.2.9 , . 3.2.10 , . 3.2.11 , ( , ). 3.2.12 , . 3.2.13 ,

5、 . 3.2.14 , , . 3.3 3.3.1 (Haematocrit) 3.3.2 3.3.3 4 4.1 . , , , , , KS I ISO 238931:2009 4 ( ) , , ( ) . . , . 4.2 1 . , , (3.1.4) (3.1.5) . 1 . 9 , 11 . . ( , , , ) . 4.3 , . . . . , . (biotope) . , (3.1.9) . 4.4 4.4.1 . . . , 20 cm 30 cm 25 . , . A B . , (10 20) . . , . , ( , ). KS I ISO 238931:

6、2009 5 4.4.2 ( 1) ( 3) . , . , (3.1.4) (3.1.5) . , (3.1.9) , 2 4 . , . , . . . ( , , ) . . . 20 cm 30 cm 30 mm 33 mm . 2 , . . 2 4 , 3 5 . 12 . . . C . 4.4.3 (3.2.2) (3.1.9) . 100 m . (3.2.3) , . , . , (3.2.4) MS-222(tricaine methanesulfonate ethyl 3-aminobenzoate methanesulfonate) 1) (3.2.5) . , ,

7、, , , ( 1 ). 1) MS-222 . ISO . Tricaine methanesulfonate . 1：1 000 5 10 . quinaldine(2-methylquinoline) , 1：20 000 5 10 , 5 mL 0.2 g benzocaine(ethyl 4-aminobenzoate) ( ) 8 L ( 11). .KS I ISO 238931:2009 6 1 2 3 4 5 6 7 8 1 , . 1) 1 g , 1 mm . 2) ( 1 ). 3) . 4) . 5) ( ). 6) , 0.1 g . 7) , 0.1 g . 8)

8、 , 0.1 g . 9) . 10) . 11) ( , ) . 12) . 13) . ( ) (subsamples) . . ( , ) KS I ISO 238931:2009 7 . . . . , , . . , . , (3.2.11) , . . . 10 . 2 , 3 4 . A D . 4.5 70 . ( , ) , . . , . . 4.6 , . . , . , (, ) . 5 5.1 KS I ISO 238931:2009 8 . , . , ( ) . 1 . 5.2 () . . , . 5.3 . 10 2 , 3 4 . , / , . 5.4 /

9、 . . 5.5 . . 6 6.1 1 . ( ) / . , . . C D . 6.2 , KS I ISO 238931:2009 9 1 . 6.3 . , (ANOVA ) . , / . , , / . , . B . KS I ISO 238931:2009 10 A () A.1 . . . A.2 EROD (lipid-soluble) . . P450 (cytochrome P450 monooxygenases)( , MFO .) . P450 . P450 1A . , (planar PCBs), . EROD . EROD . , 40 km . A.3 . , . . . . . . . A.4 . ( ), ( ) 2 . , , . 2 . 2 . KS I ISO 238931:2009 11 a) , ( , ) , PCBs, (DDT) . , . b) . . A.5 () . . (Na ) (Cl ) , . , (K ), (Ca 2 ) (PO ) . . , . ,