1、 KS I ISO 14442 KSKSKSKS SKSKSKS KSKSKS SKSKS KSKS SKS KS , , KS I ISO 14442 : 2007 (2012 ) 2007 11 2 http:/www.kats.go.krKS I ISO 14442：2007 : ( ) ( ) ( ) () ( ) : (http:/www.standard.go.kr) ： ：2001 12 18 ：2007 11 2 ：2012 12 27 ： 20120787 ： ( 02-509-7270) (http:/www.kats.go.kr). 10 5 , . KS I ISO 1
2、4442：2007 .1 1 1 2 2 3 .2 4 3 4.1 3 4.2 3 4.3 4 4.4 5 4.5 .6 5 6 5.1 .6 5.2 .7 5.3 7 6 8 7 .8 7.1 .8 7.2 9 7.3 9 7.4 .9 8 (environmental aqueous) 10 9 11 10 11 10.1 .11 10.2 12 11 pH .13 12 13 15 i KS I ISO 14442 : 2007 (2012 ) , , Water quality Guidelines for algal growth inhibition tests with poor
3、ly soluble materials, volatile compounds, metals and waste water 2006 2 ISO 14442, Water qualityGuidelines for algal growth inhibition tests with poorly soluble materials, volatile compounds, metals and waste water , . . . . . 1 KS M ISO 8692 KS M ISO 10253 . . . a) (poorly soluble) b) c) d) e) f) g
4、) . (water-soluble and water-accommodated fractions) KS I ISO 14442：2007 . . 2 . . ( ) . KS M ISO 566716, 16： KS M ISO 8692, KS M ISO 10253, 3 . . , . , (log P ow ), , , . (7. ) (EC) . (media) . KS M ISO 566716 . (batch) , (culture) ., , . . . a) (media) b) c) . d) , (silanization). . e) , , . , / /
5、 ( , , ) , 2 KS I ISO 14442：2007 . . . , , , , , , , , , , , , , , , (COD) (TOC) . , . ( ) , . 4 4.1 . 100 mg/L . ( .) , ( .) . 1 mg/L 10 mg/L ( ) . . . ( ) . 3 100 mg/L ：“ ” 1 mg/L ：“ ” KS M ISO 566716 . . 4.2 1 mg/L 100 mg/L , . , ( , , 5.1 ) . 20 , , 3 . . , . , . (0.45 m ) . . . 3 KS I ISO 14442
6、：2007 . . , . . , , ( , 1 ) ) (20 kHz 60 W .) ( , 2 10 ) . . , ( ) , ( , Schleicher & Schll 604 2) ) . , . . 4.3 10 mg/L . , . 1 mg/L , (4.2 ) 4.2 . . a) . b) . c) . d) . e) . f) . KS M ISO 10253 KS M ISO 8692 100 L/L . 100 mL 10 L . t . t . (DMSO) . 1 mL/L( 3) . , 100 L/L . 1) Ultra Turrax . ISO .
7、2) Schleicher & Schll 604 . ISO . 4 KS I ISO 14442：2007 , . . . . . , , . ( , ) . , . 18 . 4.4 (stock) . , . , (formulations) , , . (formulated) . . a) 100 mg/L ( ) . b) 3 . c) . . (polyoxyethylene ethers) 3) (alkyl polyoxyethylene sorbitan) 4) (alkyl sorbitan) 5) 4.2 . 100 mg/L . . . 4.3 . 3) Brij
8、56 . ISO . 4) Tween 80 . ISO . 5) Span 20 . ISO . 5 KS I ISO 14442：2007 . 4.5 , . , . , . ( , ) . ( , ) , . , . ( 5) (biomass) . . ( , ) . . ( , BOD 5 /COD 6) 0.5 ) ( , 10 mg/L 100 mg/L ) . ( , ). EC (12. ). 5 5.1 / ( , , ) (formulated product) (drilling) 3 . / 2 (WAFs) . WAF . . , (WSFs) . () , WAF
9、 , . WAF 4.4 . 6) BOD , COD 6 KS I ISO 14442：2007 5.2 WAF . WAFs , (loading rate) . WAF . EL 50 EL 10 , L . 5.2 (WAFs) . WAF ( ). ( ) WAF . (headspace) . PTFE , . , . ( ) . (port) . 1/3 . . . . , WAFs . WAFs ( ) . , 20 24 WAF . , 1 4 . (WAF) . . WAFs . 5.3 WAFs . WAFs (10 g/L .) . (4.5 ) . WSFs WAFs
10、 . , . 7 KS I ISO 14442：2007 ( ) , (4.5 ). 6 , , , . WSFs 5.1 5.2 . / OECD 11 . WSF . , . 20 ( ) . . / . ( , 1 000 mg/L ) () . . 10. . , , ( ) . ( , ) (5. ) . , 1 mg/L . WSF . , WSF . ( , 16, 17) WSF . 7 7.1 , H 1 Pam 3 /mol H 100 Pam 3 /mol . , (system) . (closed bottle) , 6 . . (headspace) 6 . 8 KS I ISO 14442：2007 pH . (open test) . . 1 2 % 7 . , 0.3 g/L (NaHCO 3 ) , pH 7.0 pH ( 6). , . EC . 7.2 20300 mL (vial) . . 7.3
