1、 KSKSKSKS KSKSKSK KSKSKS KSKSK KSKS KSK KS KS I ISO 14255 , KS I ISO 14255 :2009 2009 12 18 http:/www.kats.go.krKS I ISO 14255:2009 : ( ) ( ) ( ) : (ISO/TC190) () ( ) ( ) : (http:/www.standard.go.kr) ： ：1999 12 28 ：2009 12 18 2009-0830 ： ：(ISO/TC190) ( 02-509-7266) (http:/www.kats.go.kr). 10 5 , . K
2、S I ISO 14255:2009 , Soil qualityDetermination of nitrate nitrogen, ammonium nitrogen and total soluble nitrogen in air-dry soils using calcium chloride solution as extractant 1998 1 ISO 14255, Soil qualityDetermination of nitrate nitrogen, ammonium nitrogen and total soluble nitrogen in air-dry soi
3、ls using calcium chloride solution as extractant , . 1 0.01 mol/L (, , ) . KS I ISO 11464 . 2 . . ( ) . KS I ISO 11464, KS I ISO 11465, () KS M ISO 3696, 3 0.01 mol/L (CaCl 2 ) ( 1 2 ). ( ) . . ( ) . KS I ISO 14255:2009 2 () . 1 . 2 . ( 3 ). 3 . . 4 KS I ISO 11464 2 mm . KS I ISO 11465 . 5 5.1 (201)
4、 0.01 mol/L (CaCl 2 ) 1 ：10 (m/V ) . (2) . 1 20 ( 4 ). . . 2 . 20 mL . 5.2 . 5.2.1 , 25 0.2 mS/m (KS M ISO 3696 2 ). 5.2.2 , c(CaCl 2 )0.01 mol/L, 2(CaCl 2 2H 2 O) 1.47 g (5.2.1) 1 L . CaCl 2 2H 2 O . . 5.3 5.3.1 , 10 mg . 5.3.2 , 250 mL . KS I ISO 14255:2009 3 5.3.3 , 150 r/min250 r/min . 5.3.4 , .
5、 5.3.5 , 100 mL . 5.4 10.00 g (5.3.2) . 20 (5.2.2) 100.0 mL . 20 2 (5.3.3) . (5.3.5) , 10 3 000 g (5.3.4). 6., 7., 8. 9. (), ( ), . (5.3.2) (5.2.2) . 1 . 4 , 1 . 6 6.1 (segmented flow analysis, SFA) . . . - ( -naphthylethylenediamine dihydrochloride) (sulfanilamide) 543 nm . 1 , . 2 Griess-llosvay .
6、 . . , . 6.2 6.2.1 (wetting agent), (polyoxyethylene lauryl ether, 30 %) 6 . 6.2.2 1 L (NH 4 Cl) 25 g . w(NH 4 OH) 3 % 12.5 mL (6.2.1) 1 mL . . KS I ISO 14255:2009 4 pH pH 68 . 6.2.3 Cd/Cu ( 0.3 mm0.8 mm) 5 g c(HCl)1 mol/L 1 . . (II) (CuSO 4 )20 g/L 50mL 3 . 10 . Cd/Cu . 6.2.4 Cd/Cu U (6.3.2) (6.2.2
7、) , . (6.2.3) . . 5 mm . SFA . Cd/Cu Cd/Cu . 6.2.5 1 L 0.5 L w(H 3 PO 4 )85 % 150 mL . - (C 12 H 16 N 2 Cl 2 ) 0.5 g . (C 6 H 8 N 2 O 2 S) 10 g . 6.3 6.3.1 (SFA) , , , , , . (SFA) A.1 . . 6.3.2 Cd/Cu 15 cm 2 mm U , SFA (ferrule) . SFA (6.2.3 6.2.4 ). SFA ( A.2 ). 7 7.1 . (Berthelot) 5, 6 , ( ) ( ) .
8、 KS I ISO 14255:2009 5 660 nm . . 7.2 7.2.1 , pH 5.2 1 L (Na 3 C 6 H 5 O 7 ) 24 g (KNaC 4 H 4 O 6 ) 33 g . . (6.2.1) 1 mL . 7.2.2 800 mL 1 L (C 7 H 5 O 3 Na) 80 g (NaOH) 25 g . . 7.2.3 () (2) Na 2 Fe(CN) 5 NO2H 2 O 1 g 1 L . 7.2.4 (isocyanurate) 2(Cl 2 C 3 N 3 NaO 3 2H 2 O) 2 g (NaOH) 25 g 1 L . 7.3
9、 7.3.1 , , , , . A.3 . . 8 8.1 (SFA) (K 2 S 2 O 8 ) pH 4 . UV . , ( 6. ). 1 . 2 50 mL/L pH . . 8.2 8.2.1 KS I ISO 14255:2009 6 200 mL 1 L 4 (10)(Na 2 B 4 O 7 10H 2 O) 38 g . (NaOH) 4 g . . 8.2.2 500 mL 1 L (K 2 S 2 O 8 ) 52 g . 24 . . . 8.2.3 1 L (NH 4 Cl) 25 g . (NH 4 OH)(25 %) 1 mL (6.2.1) 1 mL .
10、8.2.4 Cd/Cu 6.2.4 . 8.2.5 1 L 800 mL w(H 3 PO 4 )85 % 150 mL . (C 6 H 8 N 2 O 2 S) 10 g, 2 (EDTA) (C 10 H 14 N 2 O 8 Na 2 2H 2 O) 1 g N-(1-)(C 12 H 16 Cl 2 N 2 ) 0.5 g . . 8.3 8.3.1 , , , , . (SFA) A.4 . . 8.3.2 6.3.2 . 9 9.1 9.1.1 , c (CaCl 2 )0.1 mol/L 2(CaCl 2 2H 2 O) 14.7 g 1 L . 9.1.2 , (NO 3 N
11、)375 mg/L, (NH 4 N)200 mg/L (KNO 3 ) 2.708 3 g (NH 4 ) 2 SO 4 0.943 9 g 1 L . KS I ISO 14255:2009 7 150 . ( ), . 9.2 (9.1.2) 0 mL, 0.5 mL, 1.0 mL, 1.5 mL 2.0 mL 250 mL . (9.1.1) 25 mL . . 1 . 1 mg/L 0 0.75 1.50 2.25 3.00 0 0.40 0.80 1.20 1.60 0 1.15 2.30 3.45 4.60 5. . , . A( ) . . 10 (w N )(mg/kg)
12、(1) . 100 ) 100 ( 10 ) ( N w b a w (1) a ： , (mg/L) b ： , (mg/L) w ： KS I ISO 11465 (m/m)(%) 11 . a) b) c) (mg /g ) d) , KS I ISO 14255:2009 8 A () A.1A.4 . ：mL/min 1 2 3 S： B： ( 6.2.2) A： DB： (debubbling) C： ( 6.2.5) R1： 1, 40 cm(5 ), 1.5 mm R2： 2, 150 cm(20 ), 1.5 mm D1： 15 cm M： T： ( 6.2.4). U, 1
13、5 cm, 2 mm, D： . 50 mm, 540 nm A.1 (6.) KS I ISO 14255:2009 9 ：mL/min 1 2 S： B： ( 6.2.2) A： (segmenting air) C： ( 6.2.5) R1： 1, 40 cm(5 ), 1.5 mm R2： 2, 150 cm(20 ), 1.5 mm Dl： . 15 cm M： D： . 50 mm, 540 nm A.2 (7.1 ) KS I ISO 14255:2009 10 ：mL/min 1 2 S： B： ( 7.2.1) A： (segmented air) SS： . ( 7.2.2
14、) SN： ( 7.2.3) SD： ( 7.2.4) R1, R2, R3： . 40 cm(5 ), 1.5 mm R4： 4, 370 cm(50 ), 1.5 mm, 40 . Dl： . 30 cm M： D： . 50 mm, 660 nm A.3 (7.) KS I ISO 14255:2009 11 ：mL/min 1 2 3 4 (UV digestion) S： A： (segmenting air) O： ( 8.2.2) BB： (borax) ( 8.2.1) RS： (resampling) B： ( 8.2.3) DB： (debubbling) C： ( 8.2
15、.5) R1： . 435 cm(50 ), 1.5 mm R2, R4, R5： . 75 cm(10 ), 1.5 mm R3： . , 260 cm(23 ), 1.8 mm, . R6： . 150 cm(20 ), 1.5 mm Dl： . 15 cm M： T： ( 8.2.4). U, 15 cm, 2 mm, . D： . 50 mm, 540 nm A.4 (8.) KS I ISO 14255:2009 12 B () 1 Houba, V.J.G., Novozamsky, I., Huybregts, A.W.M. and van der Lee, J.J. Compa
16、rison of soil extractions by 0.01 M CaCl 2 , by EUF and by some conventional extraction procedures. Plant and soil, 96, 433437(1986) 2 Houba, V.J.G., Novozamsky, I. and van der Lee, J.J. Some aspects of determination of “total soluble nitrogen” in soil extracts, VDLUFASchriftenreihe, 30, 295302(1989
17、) 3 Sterckeman, T. and Ciesielsky, H. Principaux facteurs influant sur la dtermination de lazote minral des sols. Actes des Troisimes Journes de lAnalyse de Terre, GEMAS, Blois, 101121(1991) 4 Houba, V.J.G., Novozamsky, I., Uittenbogaard, J. and van der Lee, J.J. Automatic determination of “total so
18、luble nitrogen” in soil extracts, Landw. Forsch. 40, 295302(1987) 5 Krom, M. Spectrophotometric determination of ammonia：a study of modified Berthelot reaction using salicylate and dichloroisocyanurate. The Analyst, April 1980 6 Searle, P.L The Berthelot or indophenol reaction and its use in the ana
19、lysis chemistry of nitrogen, The Analyst, 109, May 1984 7 Grasshoff, K. Zur Bestimmung von Nitrat in Meer-und Trinkwasser. Kieler Meeresforsch. 20/1, 5 11(1964) 8 Wood, E.D., Armstrong, F.A.J. and Richards, F.A. Determination of nitrate in sea water by cadmium- copper reduction to nitrite. J. Marine
20、 Biology Assoc.(UK), 47, 2331(1967) 9 Aminot, A. and Chaussepied, M. Manuel des analyses chimiques en milieu marin. Centre National pour lExploitation des Ocans. 395 pp , 153787 1 92 3(13) (02)26240114 (02)262401489 http:/ KS I ISO 14255 :2009 KSKSKS SKSKS KSKS SKS KS SKS KSKS SKSKS KSKSKS Soil quality Determination of nitrate nitrogen, ammonium nitrogen and total soluble nitrogen in air-dry soils using calcium chloride solution as extractant ICS 13.080 Korean Agency for Technology and Standards http:/www.kats.go.kr
