1、 KSKSKSKSKSKSKSK KSKSKS KSKSK KSKS KSK KS KS I ISO 10712 Pseudomonas putida (Pseudomonas ) KS I ISO 10712:2008 2008 11 10 http:/www.kats.go.krKS I ISO 10712:2008 : ( ) ( ) ( ) : (http:/www.standard.go.kr) ： ：1998 4 30 ：2008 11 10 2008-0751 ： ： ( 02-509-7266) (http:/www.kats.go.kr). 10 5 , . KS I ISO
2、 10712:2008 Pseudomonas putida (Pseudomonas ) Water quality Pseudomonas putida growth inhibition test (Pseudomonas cell multiplication inhibition test) 1995 1 ISO 10712, Water quality Pseudomonas putida growth inhibition test(Pseudomonas cell multiplication inhibition test) , . 1 , , Pseudomonas put
3、ida . , , , ( , ) , . ( A ). 2 . . ( ) . KS M ISO 7027, 3 . 3.1 , 3.2 KS I ISO 10712:2008 2 1 , . 3.3 (EC) (161) h Pseudomonas putida . (EC10 EC50) 10 % 50 % (3.2 ) . 3.4 3.5 , 3.6 (3.9 ) 3.7 3.8 3.9 , , ( ) 3.10 , , 3.11 , pH , , 3.12 , , ( ) 3.13 (FNU) 436 nm KS M ISO 7027 . KS I ISO 10712:2008 3
4、4 Pseudomonas putida . . (161) h . (161) h 1050 % . 5 . 5.1 Pseudomonas putida, Pseudomonadaceae (gram-negative) . (pollar flagellation) ( 0.71.1 m, 2.04.0 m). . 2530 . 2 2 . a) MIGULA Berlin 33/2 strain(DSM 50026) . German collection of microorganisms Mascheroder Weg 1b D 38124 Braunschweig Germany
5、 b) NCIB strain 9494 . Torry Research Station P.O. Box 31 Aberdeen, UK . 5.2 c(HCl) 1 mol/L 5.3 c(NaOH) 1 mol/L pH . 5.4 IIV(5.4.15.4.4 ) 121 10 . 24 . 5.4.1 I 500 mL . (NaNO3) 10.0 g (K2HPO4) 2.40 g KS I ISO 10712:2008 4 (KH2PO4) 1.20 g 1.0 g 5.4.2 II 500 mL . (NaNO3) 10.0 g (K2HPO4) 2.40 g (KH2PO4
6、) 1.20 g 5.4.3 III, 500 mL . , D( ) (1 ) (C6H12O6 H2O) 40.0 g 5.4.4 IV, (III) 1 000 mL . (7 ) (MgSO4 7H2O) 4.0 g (III) 0.01 g 3 I III 5.5 8.1 , II III 8.2 . 5.5 ( 1 ) 5.5.1 ( ) 18 g ( ) . I(5.4.1 ) 50 mL, III (5.4.3 ) 125 mL, IV(5.4.4 ) 100 mL 1 000 mL . 610 mL 121 10 . 24 . 1 ( 5.5) mg/L ( 8.1)mg/L
7、 ( 8.2) mg/L NaNO3K2HPO4KH2PO4 C6H12O6 H2O MgSO4 7H2O (III) 1 000 240 120 100 10 000 400 1.0 18 000 500 120 60 50 2 000 200 0.5 500 120 60 2 000 200 0.5 KS I ISO 10712:2008 5 5.5.2 (5.5.1 ) Pseudomonas putida . . (25 4) 24 h (254) . . , . 4 24 . . 6 . . 6.1 . 6.2 100 6.3 pH 6.4 6.5 6.6 ( ) 7 . (24 2
8、 ) ( 18 2 ) . . , . pH . pH . pH pH 7.4 . pH (5.2 ) (5.3 ) (7.40.3) . . , . . KS I ISO 10712:2008 6 8 8.1 (3.5) 8.1.1 900 mL 900 mL . I III(5.4.1 5.4.3 ) 25 mL , IV(5.4.4 ) 50 mL . 5 pH (7.20.2) . ( , 250 mL 90 mL ). 8.1.2 7 (5.5.2 ) . (8.1.1 ) (5.5.1) . 10 FNU . 10 mL (8.1.1 ) 90 mL 100 mL 100 FNU
9、. 6 (KS M ISO 7027 3.4 ) (KS M ISO 7027 3.3 ) KS M ISO 7027 3 . 0.4 FNU . , 0.4 0.10.4 . 7 FNU . A610 0.02(10 FNU) A610 0.2(100 FNU) A610 0.1(50 FNU) A610： 610 nm 8.1.3 (8.1.2 ) (8.1.1 ) . (6.5 ) . (8.3 ) (50.5) h ( ). ( ). ( 50 FNU) ( 1 ) . 8 . . . , . KS I ISO 10712:2008 7 8.2 ( , 2 ) (3.11 ) (5.
10、) . (250 mL) 100 mL . 100 mL (mL) . II, III, IV(5.4 ) , (6.5 ) . 8.1.2 5 FNU . . 80 % . , 3 . , , . 9 , . . (8.1.1 ) . 2 ( 5.4) f 2 ( 5.)mL mL II mL III mL IV mL ( 8.1.2) (50 FNU, 436 nm) mL mL 2 4 8 16 32 64 80 30 55 67.5 73.7 76.9 78.5 0 50 25 12.5 6.3 3.1 1.5 2.5 2.5 2.5 2.5 2.5 2.5 2.5 2.5 2.5 2
11、.5 2.5 2.5 2.5 2.5 5 5 5 5 5 5 5 10 10 10 10 10 10 10 100 100 100 100 100 100 100 8.3 (231) . 1 . ( , ) . 8.4 (161) h . 10 . , . . 9 KS I ISO 10712:2008 8 . (5 FNU) 60 3,5 EC50 10 mg/L 30 mg/L 11 60 5 6 . 10 ( , 3 ) . (I) . 1000ccBBBBIn I ： Bn ： n ( 1： 2) Bc ： B0 ： t0 (I) . EC10 EC50 10 % 50 % . 12
12、, . 3 FNU(436 nm) (f 2) 1 2 3 FNU(436 nm) 2 1 4 1 8 1 16 1 32 1 64 1 23 58 128 290 426 455 25 64 131 279 426 460 24 64 139 282 403 450 24 62 133 284 418 455 ： 440 FNU, 448.8 FNU, 439 FNU, 460 FNU, 455 FNU, 450 FNU(436 nm) 11 ( 4 ). KS I ISO 10712:2008 9 4 % 2 4 8 16 32 64 95 86 70 37 8 0 12 . a) b)
13、/ , c) ( ) , , pH , , d) e) pH f) ( ) g) (11. ) ( ) EC10 EC50 ( ) EC h) , 13 . , . 14 1989 21 (round-robin) 3,5 EC . EC10： 13.7 mg/L EC50： 21.4 mg/L (VCR) 31.8 % 23 % . KS I ISO 10712:2008 10 A ( ) . . . 2 (5. ) . , 50 % (EC50) . . ( , 3.2 ) (10. ) (I) . EC50 . . (batch) KS I ISO 10712:2008 11 B ( )
14、 1 BRINGMANN, G. and KHN, R Limiting values for the damaging action of water pollutants to bacteria(Pseudomonas putida) and green algae(Scendesmus quadricauda) in the cell multiplication inhibition test. Z.f. Wasser-und Abwasser-Forschung 10(1977), pp.8798 Pseudomonas putida ( Pseudomonas ) 135513 7017 (02)60094114 (02)600948878 http:/ Korean Agency for Technology and Standards http:/www.kats.go.kr KS I ISO 10712:2008 KSKSKS SKSKS KSKS SKS KS SKS KSKS SKSKS KSKSKS Water qualityPseudomonas putida growth inhibition test(Pseudomonas cell multiplication inhibition test) ICS 13.060.40
