1、 , ()INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION (ISC) ISO10727null2013 . (ISO 10727:2002, IDT) ISO 107272013II, - 1.092 . - 1.22009 . -, . , -, , 1 ( ) 5 , 2 ()3 , ( 25 2013 . 55-) : ( 3166) 00497 ( 3166) 00497 AM BY KG MD - RU TJ UZ 4 28 2013 . 355- ISO 107272013 1 2014 .5
2、ISO 10727:2002 Tea and instant tea in solid form Determination of caffeine content Method using high-performance liquid chromatography ( . . - ). ISO TC 34/SC 8 ISO/TC 34 (ISO). (en). , , . - 1.52001 ( 3.6). - . ( IDT)6 ISO 107272013III , 2014 , - - , - . () - - . , ISO 1072720131 . 12 . 13 14 . 15
3、. 26 . . 37 . . 38 . 39 . 410 411 . 5 A () . . 6 () . 7 8IV1 201401011 (). , . . . 2 , . . , . ISO IEC . ISO 1572:1980 Tea; Preparation of ground sample of known dry matter content (. ) ISO 3696:1987 Water for analytical laboratory use; Specification and test methods ( . ) ISO 7513:1990 Instant tea
4、in solid form; determination of moisture content (loss in mass at 103 null ) ( . ( 103 C) 3 . - - . 4 . 4.1 ISO 3696. 4.2 . 4.3 , . 600 3 (. 4.2) 2 3. . 0,45 (. 5.3). ISO 107272013 . Tea and instant tea. Determination of caffeine content. Method using high-performance liquid chromatography ISO 10727
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6、, . , 280 , . . 272 . , , .5.2 C18 ( 5000 )*. N , (. 4.7), t ; W0,5 . NtW=554052,(1)*Spherisorb 5 ODS, Spherisorb 10 ODS, Nucleosil 5 C18, Nucleosil 7 C18, Nucleosil 10 C18, Zorbax BP C18, Hypersil ODS, CP-Sper C18, Bondapak C18, Supelcosil L C18 Partisphere C18 , . ISO. ISO 1072720133 (. 4.3) Parti
7、sphere C18 110 x 4,6 , Whatman. , . 5.3 0,45 . 5.4 , 1 3. 5.5 1 3, . 5.6 . 5.7 , , 1 3. 5.8 0,001 . 6 , . . 1 , 2 . 7 ISO 1572. . 8 8.1 , . 8.2 8.6 . 8.2 , 103 C ISO 1572 , ISO 7513 - . 8.3 8.3.1 (. 5.5) 1,0 (. 7) 0,001 . 8.3.2 (. 5.5) 0,5 (. 7) 0,001 . 8.4 8.4.1 , , (4,5 0,5) (. 4.5), (. 5.6) 300 3
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