1、, (ISO 13904:2005, MOD) , ()INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION(ISC)322012013(ISO 13904:2005), - 1.092 . 1.22009 . -, . , , 1 - ( )2 (-)3 , ( 14 2013 . 44) : ( 3166) 00497 ( 3166) 00497 AMRUUZ 4 ISO 13904:2005 Animal feeding stuffs Determination of tryptophan content
2、( . - ). ISO/TC 10 - ISO/TC 34 - (ISO). (en). , , 1.52001, - . , , . : , , 1.52001( 4.14.1). - . , - , - . . (MOD)5 22 2013.1698-322012013(ISO13904:2005) 1 2015 .6 II 322012013 -, - . () . , - - , 2014 -, III 3220120131 .12 13 .24 .25 26 37 48 49 .410 511 .512 .6() 7 () 8() .1012IV 322012013, Feeds,
3、 compound feeds. Method for determination of tryptophan1 , , , , - ( ) . D- L- .2 -: 6175 . . 177074 ( 104283, 478880) ., , , . 311877 . . 410778 . 8-. 432877 . . 655280 . . 699577 . -. 13496.080* , . 241042001* . 2533682 . , - 2922791 ( 835-181) . -. 1. - - , 1 , . (), - () . , , , , .* 64972011.*
4、532282008.1 322012013(ISO 13904:2005) 201507013 110 C 20 . - . - . - () .4 4.1 .4.2 125 c180 4 , C18 3 .4.3 -.4.4 125 3 .4.5 0,45 .4.6 , (110 c177 2) C (140 c177 10) (1,4 c177 0,1) . (. 4.9) , (110 c177 2) C.4.7 .4.8 -.4.9 , (110 c177 2) C.4.10 1(2)-50(100, 500, 1000) 2 1770.4.11 24104 200 - c1770,0
5、001 .4.12 1(2, 3, 5)-1(1, 2, 2)-1-1(2, 5, 10) 29227.4.13 -2-250- 25336.4.14 ()-1(2)-50(1000) 25336.4.15 0,5 . , , , 5 5.1 , (10/).5.2 ( 99 %), .5.3 : c97- ( 99 %), .5.4 8- 4107, . . Ba(OH)2c215 8H2O , BaCO3, (. .3).5.5 4328.5.6 85 %.5.7 3118, ,c11420=1,19/3.5.8 6995, .5.9 , 40C60C.5.10 61.5.11 98 %.
6、2 3220120135.12 1,1,1-2-2-. -, , .6 6.1 1/3 1000 3(. 4.10) 40,0 (. 5.5), (. 5.1) .6.2 6/3 1000 3(. 4.10) 492 3 (. 5.7) (. 5.1).6.3 1/3 1000 3(. 4.10) 82 3 (. 5.7)- (. 5.1).6.4 0,1 /3 1000 3(. 4.10) 8,2 3 (. 5.7)- (. 5.1).6.5 0,5 /3 1000 3(. 4.10) 34 3 (. 5.6) (. 5.1).6.6 0,5 /3 500 3(. 4.10) (0,2500
7、 c177 0,0001) (. 5.2), 0,1 /3(. 6.4) - . 18 C .6.7 0,54 /3 500 3(. 4.10) (0,2700 c177 0,0001) - (c97-) (. 5.3), 0,1 /3(. 6.4) - . 18 C .6.8 2,00 3 (.6.6) 2,00 3 - (c97-) (. 6.7) (. 5.1)(.5.8)(10%30%). . .6.9 1,1,1-2-2- 100 3(. 4.10) 1 1,1,1-2-2- (. 5.12) (. 5.8).6.10 1000 3(. 4.14) 900 3 (. 5.1), 3,
8、00 (. 5.10) 50 3 1,1,1-2-2-(. 6.9). pH 5,00 (. 5.11), 1000 3(. 4.10) .3 3220120137 13496.0.8 (. 4.15). - 50 C -, (. 5.9).9 9.1 () (. 4.11) (1,000 c177 0,001)(5,000 c177 0,001), 8, (. 4.13). 100 3 (. 6.4) 5,00 3 - (. 6.8). 60 (. 4.7). , (. 4.12) 10,0 3 (. 4.14). 5 3 (. 5.6) 3,0 . (. 6.1). (. 5.8), 10 % 30 % . -(.5.1),- , (. 6.8). - (. 4.5). - 9.3. . - , 5 C.9.2 ()
