1、BS ISO 14189:2013BS EN ISO 14189:2016Water quality Enumeration of Clostridium perfringens Method using membrane filtration (ISO 14189:2013)BSI Standards PublicationWB11885_BSI_StandardCovs_2013_AW.indd 1 15/05/2013 15:06BS EN ISO 14189:2016 BRITISH STANDARDNational forewordThis British Standard is t
2、he UK implementation of EN ISO 14189:2016. It is identical to ISO 14189:2013. It supersedes BS ISO 14189:2013 which is withdrawn.The UK participation in its preparation was entrusted by Technical Committee EH/3, Water quality, to Subcommittee EH/3/4, Microbiological methods.A list of organizations r
3、epresented on this subcommittee can be obtained on request to its secretary.This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. The British Standards Institution 2016. Published by BSI Standards Limited 2016ISBN
4、978 0 580 92184 1ICS 07.100.20Compliance with a British Standard cannot confer immunity from legal obligations.This British Standard was published under the authority of the Standards Policy and Strategy Committee on 30 November 2013.Amendments/corrigenda issued since publicationDate Text affected31
5、 August 2016 This corrigendum renumbers BS ISO 14189:2013 as BS EN ISO 14189:2016.EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 14189 August 2016 ICS 07.100.20 English Version Water quality - Enumeration of Clostridium perfringens - Method using membrane filtration (ISO 14189:2013) Qualit
6、 de leau - Dnombrement de Clostridium perfringens - Mthode de filtration sur membrane (ISO 14189:2013) Wasserbeschaffenheit - Zhlung von Clostridium perfringens - Verfahren mittels Membranfiltration (ISO 14189:2013) This European Standard was approved by CEN on 15 July 2016. CEN members are bound to
7、 comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CE
8、NELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has t
9、he same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxem
10、bourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brusse
11、ls 2016 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 14189:2016 EBS ISO 14189:2013ISO 14189:2013(E) ISO 2013 All rights reserved iiiContents PageForeword ivIntroduction v1 Scope . 12 Normative references 13 Terms and definit
12、ions . 14 Principle 25 Apparatus and glassware 26 Culture media and reagents 36.1 Basic materials 36.2 Culture media . 37 Sampling 38 Procedure. 38.1 Transport and storage of the sample . 38.2 Heat pre-treatment to select spores . 38.3 Sample dilution . 38.4 Filtration . 48.5 Incubation and examinat
13、ion . 48.6 Confirmation of Clostridium perfringens . 49 Expression of results 510 Test report . 511 Quality assurance 5Annex A (normative) Composition and preparation of culture media and reagents 7Annex B (informative) Performance data . 9Bibliography .12EN ISO 14189:2016 (E) 3 European foreword Th
14、e text of ISO 14189:2013 has been prepared by Technical Committee ISO/TC 147 “Water quality” of the International Organization for Standardization (ISO) and has been taken over as EN ISO 14189:2016 by Technical Committee CEN/TC 230 “Water analysis” the secretariat of which is held by DIN. This Europ
15、ean Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by February 2017, and conflicting national standards shall be withdrawn at the latest by February 2017. Attention is drawn to the possibility that some of the el
16、ements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this
17、European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia,
18、 Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. Endorsement notice The text of ISO 14189:2013 has been approved by CEN as EN ISO 14189:2016 without any modification. BS EN ISO 14189:2016EN ISO 14189:2016 (E)BS ISO 14189:2013ISO 14189:2013(E) ISO 2013 All rights reserved iiiCont
19、ents PageForeword ivIntroduction v1 Scope . 12 Normative references 13 Terms and definitions . 14 Principle 25 Apparatus and glassware 26 Culture media and reagents 36.1 Basic materials 36.2 Culture media . 37 Sampling 38 Procedure. 38.1 Transport and storage of the sample . 38.2 Heat pre-treatment
20、to select spores . 38.3 Sample dilution . 38.4 Filtration . 48.5 Incubation and examination . 48.6 Confirmation of Clostridium perfringens . 49 Expression of results 510 Test report . 511 Quality assurance 5Annex A (normative) Composition and preparation of culture media and reagents 7Annex B (infor
21、mative) Performance data . 9Bibliography .12BS EN ISO 14189:2016ISO 14189:2013(E)BS ISO 14189:2013ISO 14189:2013(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standard
22、s is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take
23、part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In parti
24、cular the different approval criteria needed for the different types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).Attention is drawn to the possibility that some of the elements of th
25、is document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the document will be in the Introduction and/or on the ISO list of patent declarations received (see www
26、.iso.org/patents).Any trade name used in this document is information given for the convenience of users and does not constitute an endorsement.For an explanation on the meaning of ISO specific terms and expressions related to conformity assessment, as well as information about ISOs adherence to the
27、 WTO principles in the Technical Barriers to Trade (TBT) see the following URL: Foreword - Supplementary informationThe committee responsible for this document is ISO/TC 147, Water quality, Subcommittee SC 4, Microbiological methods.iv ISO 2013 All rights reservedBS ISO 14189:2013ISO 14189:2013(E)In
28、troductionClostridium perfringens is widely recognized as a valuable indicator for faecal pollution. Within the intestinal tract of animals and man, these Gram-positive bacteria form spores which are resistant to heating compared with vegetative cells. C. perfringens in the intestine exists both as
29、spores and vegetative cells, spores are also found in environmental samples. The spores of C. perfringens survive in water for months, much longer than vegetative faecal indicator bacteria and consequently their presence may indicate remote or intermittent faecal pollution. Monitoring of C. perfring
30、ens has proven useful for the assessment of the quality of water resources and to check the stages of water treatment to evaluate the treatment-works performance. The spores are not always inactivated by routine disinfection procedures (e.g. chlorination). ISO 2013 All rights reserved vBS EN ISO 141
31、89:2016ISO 14189:2013(E)BS ISO 14189:2013ISO 14189:2013(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committee
32、s. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the Inter
33、national Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the diff
34、erent types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shal
35、l not be held responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the document will be in the Introduction and/or on the ISO list of patent declarations received (see www.iso.org/patents).Any trade name used in this document is
36、 information given for the convenience of users and does not constitute an endorsement.For an explanation on the meaning of ISO specific terms and expressions related to conformity assessment, as well as information about ISOs adherence to the WTO principles in the Technical Barriers to Trade (TBT)
37、see the following URL: Foreword - Supplementary informationThe committee responsible for this document is ISO/TC 147, Water quality, Subcommittee SC 4, Microbiological methods.iv ISO 2013 All rights reservedBS ISO 14189:2013ISO 14189:2013(E)IntroductionClostridium perfringens is widely recognized as
38、 a valuable indicator for faecal pollution. Within the intestinal tract of animals and man, these Gram-positive bacteria form spores which are resistant to heating compared with vegetative cells. C. perfringens in the intestine exists both as spores and vegetative cells, spores are also found in env
39、ironmental samples. The spores of C. perfringens survive in water for months, much longer than vegetative faecal indicator bacteria and consequently their presence may indicate remote or intermittent faecal pollution. Monitoring of C. perfringens has proven useful for the assessment of the quality o
40、f water resources and to check the stages of water treatment to evaluate the treatment-works performance. The spores are not always inactivated by routine disinfection procedures (e.g. chlorination). ISO 2013 All rights reserved vBS EN ISO 14189:2016ISO 14189:2013(E)BS ISO 14189:2013 BS ISO 14189:20
41、13Water quality Enumeration of Clostridium perfringens Method using membrane filtrationWARNING Persons using this document should be familiar with normal laboratory practice. This document does not purport to address all of the safety problems, if any, associated with its use. It is the responsibili
42、ty of the user to establish appropriate safety and health practices and to ensure compliance with any national regulatory conditions.IMPORTANT It is absolutely essential that tests conducted in accordance with this document be carried out by suitably qualified staff.1 ScopeThis International Standar
43、d specifies a method for the enumeration of vegetative cells and spores of Clostridium perfringens by the membrane filtration method in samples of water intended for human consumption. However, the method can be applied to all types of water samples provided they do not contain particulate or colloi
44、dal matter that interferes with filtration.2 Normative referencesThe following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of th
45、e referenced document (including any amendments) applies.ISO 8199, Water quality General guidance on the enumeration of micro-organisms by cultureISO/TS 11133-1, Microbiology of food and animal feeding stuffs Guidelines on preparation and production of culture media Part 1: General guidelines on qua
46、lity assurance for the preparation of culture media in the laboratoryISO 19458, Water quality Sampling for microbiological analysisISO/IEC Guide 2:2004, Standardization and related activities General vocabulary3 Terms and definitionsFor the purposes of this document, the terms and definitions given
47、in ISO/IEC Guide 2 and the following apply:3.1presumptive Clostridium perfringensbacteria which produce all shades of black or grey to yellow brown colonies on tryptose-sulfite-cycloserine agar, even if the colour is faint, after anaerobic incubation at (44 1) C for (21 3) hNote 1 to entry: Unlike c
48、olonies growing directly on the agar medium, colonies on the membrane do not always display a distinct blackening, so faint colonies are included in the count.3.2confirmed Clostridium perfringensbacteria that produce characteristic colonies on tryptose-sulfite-cycloserine agar and possess the enzyme
49、 acid phosphataseINTERNATIONAL STANDARD ISO 14189:2013(E) ISO 2013 All rights reserved 1BS EN ISO 14189:2016BS ISO 14189:2013 BS ISO 14189:2013Water quality Enumeration of Clostridium perfringens Method using membrane filtrationWARNING Persons using this document should be familiar with normal laboratory practice. This document does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user to establish appropriate safety and health practices and to ensure c
