1、January 2012 Translation by DIN-Sprachendienst.English price group 14No part of this translation may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).IC
2、S 13.040.20!$y+E“1860834www.din.deDDIN ISO 16000-18Indoor air Part 18: Detection and enumeration of moulds Sampling by impaction (ISO 16000-18:2011 + Cor. 1:2011)English translation of DIN ISO 16000-18:2012-01Innenraumluftverunreinigungen Teil 18: Nachweis und Zhlung von Schimmelpilzen Probenahme du
3、rch Impaktion (ISO 16000-18:2011 + Cor. 1:2011)Englische bersetzung von DIN ISO 16000-18:2012-01Air intrieur Partie 18: Dtection et dnombrement des moisissures chantillonnage par impaction (ISO 16000-18:2011 + Cor. 1:2011)Traduction anglaise de DIN ISO 16000-18:2012-01www.beuth.deDocument comprises
4、28 pagesIn case of doubt, the German-language original shall be considered authoritative.This standard has been included in the VDI/DIN Handbooks on air quality, Volume 5. 02.12 A comma is used as the decimal marker. Contents Page National foreword .3 National Annex NA (informative) Bibliography .5
5、Introduction .8 1 Scope 9 2 Normative references 9 3 Terms and definitions9 4 Principle 11 5 Apparatus and materials .11 5.1 Sampling device .11 5.2 Equipment for preparing the agar plates 12 5.3 Equipment for sampling 12 6 Culture media and reagents 12 6.1 General 12 6.2 Dichloran 18 % glycerol aga
6、r (DG18) .12 6.3 Malt-extract agar 13 6.4 Potato dextrose agar .13 7 Measurement procedure .14 7.1 Preparation for sampling 14 7.2 Sampling .14 7.3 Sampling period and sampling volume .15 7.4 Transport and storage .15 8 Sampling efficiency and method limitations .16 9 Calibration of flow rate, funct
7、ion control and maintenance of the sampling system .16 9.1 Calibration of flow rate 16 9.2 Function control and maintenance of the sampling system .16 10 Quality assurance 16 11 Sampling protocol .17 12 Performance characteristics .17 Annex A (informative) Technical description of a suitable one sta
8、ge sieve impactor .18 Annex B (informative) Sampling protocol .19 Annex C (informative) Sample exchange for method validation 20 Bibliography 28 2 DIN ISO 16000-18:2012-01 DIN ISO 16000-18:2012-01 National foreword International technical regulation pertaining to indoor air This standard has been pr
9、epared by Technical Committee ISO/TC 146 “Air quality”, Subcommittee SC 6 “Indoor air”, Working Group WG 10 “Fungi” (Convenership and Secretariat: DIN, Germany). The responsible German body involved in its preparation was the Kommission Reinhaltung der Luft (KRdL) im VDI und DIN Normenausschuss (Com
10、mission on Air Pollution Prevention of VDI and DIN Standards Committee), Joint Committee Bioaerosole und biologische Agenzien. Sampling by impaction as specified in this standard is based on VDI 4300 Part 10, which deals with indoor air. The procedures as defined in the two documents are in principl
11、e identical. The VDI Guideline describes sampling methods other than the impaction method, and also describes the measurement strategy. Data relating to indoor air for the validation of the method described in this DIN ISO which were not previously available have been included here. General and subs
12、tance-specific aspects which should be taken into account before or during measurements, as well as methods for determining individual substances or substance groups, and microorganisms in indoor air are described in the various parts of ISO 16000. The DIN Standards corresponding to the Internationa
13、l Standards referred to in Clause 2 of this standard are as follows: ISO 16000-16 DIN ISO 16000-16 ISO 16000-17 DIN ISO 16000-17 Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. DIN shall not be held responsible for identifying any
14、 or all such patent rights. National technical regulation pertaining to indoor air General conditions and other aspects to be taken into consideration when planning indoor air measurements are described for individual substances and substance groups in the VDI 4300 series (see also “National Annex N
15、A”). Many of these served as the sole basis for parts of the ISO 16000 series (e.g. ISO 16000-1, ISO 16000-2, ISO 16000-5, ISO 16000-7, ISO 16000-12, ISO 16000-15, ISO 16000-16, ISO 16000-17 and ISO 16000-18). The specific procedures (sampling, analysis) are described in the VDI 4301 series for indi
16、vidual substances and substance groups. Methods which can be used for both indoor and ambient air measurements are described in further VDI guidelines. The DIN ISO 16000 series and DIN EN ISO 16000 series consist of the following parts, under the general title Indoor air: DIN EN ISO 16000-1, General
17、 aspects of sampling strategy DIN EN ISO 16000-2, Sampling strategy for formaldehyde DIN ISO 16000-3, Determination of formaldehyde and other carbonyl compounds Active sampling method DIN ISO 16000-4, Determination of formaldehyde Diffusive sampling method DIN EN ISO 16000-5, Sampling strategy for v
18、olatile organic compounds (VOCs) DIN ISO 16000-6, Determination of volatile organic compounds in indoor and test chamber air by active sampling on Tenax TAsorbent, thermal desorption and gas chromatography using MS/FID 3 DIN ISO 16000-18:2012-01 DIN EN ISO 16000-7, Sampling strategy for determinatio
19、n of airborne asbestos fibre concentrations DIN ISO 16000-8, Determination of local mean ages of air in buildings for characterizing ventilation conditions DIN EN ISO 16000-9, Determination of the emission of volatile organic compounds from building products and furnishing Emission test chamber meth
20、od DIN EN ISO 16000-10, Determination of the emission of volatile organic compounds from building products and furnishing Emission test cell method DIN EN ISO 16000-11, Determination of the emission of volatile organic compounds from building products and furnishing Sampling, storage of samples and
21、preparation of test specimens DIN EN ISO 16000-12, Sampling strategy for polychlorinated biphenyls (PCBs), polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) and polycyclic aromatic hydrocarbons (PAHs) DIN ISO 16000-13, Determination of total (gas and particle-phase) po
22、lychlorinated dioxin-like biphenyls (PCBs) and polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDDs/PCDFs) Collection on sorbent-backed filters DIN ISO 16000-14, Determination of total (gas and particle-phase) polychlorinated dioxin-like biphenyls (PCBs) and polychlorinated dibenzo-p-dioxins/diben
23、zofurans (PCDDs/PCDFs) Extraction, clean-up and analysis by high-resolution gas chromatography/mass spectrometry DIN EN ISO 16000-15, Sampling strategy for nitrogen dioxide (NO2) DIN ISO 16000-16, Detection and enumeration of moulds Sampling by filtration DIN ISO 16000-17, Detection and enumeration
24、of moulds Culture-based method DIN ISO 16000-18, Detection and enumeration of moulds Sampling by impaction DIN ISO 16000-19, Sampling strategy for moulds DIN ISO 16000-23, Performance test for evaluating the reduction of formaldehyde concentrations by sorptive building materials DIN ISO 16000-24, Pe
25、rformance test for evaluating the reduction of volatile organic compounds and carbonyl compounds without formaldehyde concentrations by sorptive building materials DIN ISO 16000-25, Determination of the emission of semi-volatile organic compounds by building products Micro-chamber method DIN EN ISO
26、16000-26, Sampling strategy for carbon dioxide (CO2) DIN ISO 16000-28, Determination of odour emissions from building products using test chambers The following parts are under preparation: DIN ISO 16000-21, Detection and enumeration of moulds Sampling from materials DIN ISO 16000-27, Determination
27、of settled fibrous dust on surfaces by SEM (scanning electron microscopy) (direct method) DIN ISO 16000-29, Test methods for VOC detectors 4 DIN ISO 16000-18:2012-01 DIN ISO 16000-30, Sensory testing of indoor air DIN ISO 16000-31, Measurement of flame retardants and plasticizers based on organophos
28、phorus compounds Phosphoric acid ester DIN ISO 16000-32, Investigation of constructions on pollutants and other injurious factors Inspections The following parts are planned: DIN ISO 16000-20, Detection and enumeration of moulds Sampling from house dust DIN ISO 16000-22, Detection and enumeration of
29、 moulds Molecular methods National Annex NA (informative) Bibliography DIN EN ISO 5492, Sensory analysis Vocabulary DIN EN ISO 8589, Sensory analysis General guidance for the design of test rooms DIN 50014, Climates and their technical application Standard atmospheres DIN EN ISO 8199, Water quality
30、General guidance on the enumeration of micro-organisms by culture DIN EN ISO 9169, Air quality Definition and determination of performance characteristics of an automatic measuring system DIN ISO 12219-1, Indoor air of road vehicles Part 1: Whole vehicle test chamber Specification and method for the
31、 determination of volatile organic compounds in cabin interiors (under preparation) DIN ISO 12219-2, Indoor air of road vehicles Part 2: Screening method for the determination of the emissions of volatile organic compounds from vehicle interior parts and materials Bag method (under preparation) DIN
32、ISO 12219-3, Indoor air of road vehicles Part 3: Screening method for the determination of the emissions of volatile organic compounds from vehicle interior parts and materials Micro-chamber method (under preparation) DIN ISO 12219-4, Indoor air of road vehicles Part 4: Method for determination of t
33、he emissions of volatile organic compounds from car trim components Small chamber method (under preparation) DIN ISO 12884, Ambient air Determination of total (gas and particle phase) polycyclic aromatic hydrocarbons Collection on sorbent-backed filters with gas chromatographic/mass spectrometric an
34、alysis DIN V ENV 13005, Guide to the expression of uncertainty in measurement DIN EN 14412, Indoor air quality Diffusive samplers for the determination of concentrations of gases and vapours Guide for selection, use and maintenance 5 DIN ISO 16000-18:2012-01 DIN EN ISO 16017-1, Indoor, ambiant and w
35、orkplace air Sampling and analysis of volatile organic compounds by sorbent tube/thermal desorption/capillary gas chromatography Part 1: Pumped sampling DIN EN ISO 16017-2, Indoor, ambient and workplace air Sampling and analysis of volatile organic compounds by sorbent tube/thermal desorption/capill
36、ary gas chromatography Part 2: Diffusive sampling DIN ISO 16362, Ambient air Determination of particle-phase polycyclic aromatic hydrocarbons by high performance liquid chromatography DIN EN ISO/IEC 17025, General requirements for the competence of testing and calibration laboratories VDI 2464 Part
37、1, Ambient air measurement Indoor air measurement Measurement of polychlorinated biphynyls (PCBs) GC/MS method for PCB 28, 52, 101, 138, 153, 180 VDI 2464 Part 2, Ambient air measurement Indoor air measurement Measurement of polychlorinated biphenyls (PCBs) HR-GC/HR-MS method for coplanar PCBs VDI 2
38、464 Part 3, Ambient air measurement Indoor air measurement Measurement of polybrominated diphenylether Hexabromocyclododecane and hexabromobenzene VDI 3484 Part 1, Gaseous ambient air measurements Indoor-air pollution measurements Test gases measurements Measurement of the formaldehyde concentration
39、 with the sulfite pararosaniline method VDI 3484 Part 2, Gaseous ambient air measurements Indoor-air pollution measurements Measurement of the formaldehyde concentration with the acatylacetone method VDI 3498 Part 1, Ambient air measurement Indoor air measurement Measurement of polychlorinated diben
40、zo-p-dioxins and dibenzofurans Method using large filters VDI 3498 Part 2, Ambient air measurement Indoor air measurement Measurement of polychlorinated dibenzo-p-dioxins and dibenzofurans Method using small filters VDI 4252 Part 2, Measurement of airborne microorganisms and viruses in ambient air A
41、ctive sampling of bioaerosols Separation of airborne mould on gelatine/polycarbonate filters VDI 4253 Part 2, Measurement of airborne microorganisms and viruses in ambient air Culture based method for the determination of the concentration of mould in air Indirect method after sampling with gelatine
42、/polycarbonate filters VDI 4300 Part 1, Indoor-air pollution measurement General aspects of measurement strategy VDI 4300 Part 2, Indoor-air pollution measurement Measurement strategy for polycyclic aromatic hydrocarbons (PAHs), polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofuran
43、s (PCDFs) and polychlorinated biphenyls (PCBs) VDI 4300 Part 4, Indoor air pollution measurement Measurement strategy for pentachlorophenol (PCP) and -hexachlorocyclohexane (lindane) in indoor air VDI 4300 Part 5, Indoor air pollution measurement Measurement strategy for nitrogen dioxide (NO2) VDI 4
44、300 Part 6, Measurement of indoor air pollution Measurement strategy for volatile organic compounds (VOCs) VDI 4300 Part 7, Indoor-air pollution measurement Measurement of indoor air change rate VDI 4300 Part 8, Measurement of indoor air pollution Sampling of house dust VDI 4300 Part 9, Measurement
45、of indoor air pollution Measurement strategy for carbon dioxide (CO2) 6 DIN ISO 16000-18:2012-01 VDI 4300 Part 10, Measurement of indoor air pollution Measurement strategies for determination of mould fungi in indoor environment VDI 4301 Part 1, Indoor air pollution measurement Determination of the
46、concentration of nitrogen dioxide Manual photometric method (Saltzman) VDI 4301 Part 2, Indoor air pollution measurement Measurement of pentachlorophenol (PCP) and -hexachlorocyclohexane (lindane) GC/MS-method VDI 4301 Part 3, Measurement of indoor air pollution Measurement of pentachlorophenol (PCP
47、) and -hexachlorocyclohexane (lindane) GC/ECD method VDI 4301 Part 4, Measurement of indoor air pollution Measurement of pyrethroids and piperonyl butoxide in air, house dust and solvent wipe samples VDI 4301 Part 5, Measurement of indoor air pollution Measurement of flame retardants and plasticizer
48、s based on phosphor organic compounds Phosphoric acid ester VDI 4301 Part 6, Measurement of indoor air pollution Measurement of phthalates The following VDI Guidelines are under preparation: VDI 4300 Part 11, Measurement of indoor air pollution Measurement strategies for determination of airborne particles in indoor environmen
