欢迎来到麦多课文档分享! | 帮助中心 海量文档,免费浏览,给你所需,享你所想!
麦多课文档分享
全部分类
  • 标准规范>
  • 教学课件>
  • 考试资料>
  • 办公文档>
  • 学术论文>
  • 行业资料>
  • 易语言源码>
  • ImageVerifierCode 换一换
    首页 麦多课文档分享 > 资源分类 > PDF文档下载
    分享到微信 分享到微博 分享到QQ空间

    DIN EN 26461-2-1993 Water quality detection and enumeration of the spores of sulfite-reducing anaerobes (clostridia) part 2 method by membrane filtration (ISO 6461-2 1986) german v.pdf

    • 资源ID:673366       资源大小:761.21KB        全文页数:16页
    • 资源格式: PDF        下载积分:10000积分
    快捷下载 游客一键下载
    账号登录下载
    微信登录下载
    二维码
    微信扫一扫登录
    下载资源需要10000积分(如需开发票,请勿充值!)
    邮箱/手机:
    温馨提示:
    如需开发票,请勿充值!快捷下载时,用户名和密码都是您填写的邮箱或者手机号,方便查询和重复下载(系统自动生成)。
    如需开发票,请勿充值!如填写123,账号就是123,密码也是123。
    支付方式: 支付宝扫码支付    微信扫码支付   
    验证码:   换一换

    加入VIP,交流精品资源
     
    账号:
    密码:
    验证码:   换一换
      忘记密码?
        
    友情提示
    2、PDF文件下载后,可能会被浏览器默认打开,此种情况可以点击浏览器菜单,保存网页到桌面,就可以正常下载了。
    3、本站不支持迅雷下载,请使用电脑自带的IE浏览器,或者360浏览器、谷歌浏览器下载即可。
    4、本站资源下载后的文档和图纸-无水印,预览文档经过压缩,下载后原文更清晰。
    5、试题试卷类文档,如果标题没有明确说明有答案则都视为没有答案,请知晓。

    DIN EN 26461-2-1993 Water quality detection and enumeration of the spores of sulfite-reducing anaerobes (clostridia) part 2 method by membrane filtration (ISO 6461-2 1986) german v.pdf

    1、This standard, togethmi with DIN ENSEC! Par? 11 April la98 edition, supw- sedes DIM 3411 7, November 19636 edition. Ametsdmeints In cmpariSw71 with BlNl3841fl krt 7, Wccclember 192% editioni, detailh af tke methml (prucedluw and evalwatiotiorr ) haue ben changed. Tihis EurapeaniStan dlefediorr arrd

    2、enumerationi ofi tk spores; af sulfite-raiucirrg anmrabes (alostridia); method by membrsrre-filtration was rmomrnendii by CEN/TecImic Switzevianldarrd Whirted Mirrgdom. 1 scope Piige4- EFd 2-64H-2:7993 Measurerneiaits af pH hais be Made using a pti tmtef, measurements. b. 44: +: 4 KG. If arr arraemb

    3、icijas on am amawmbicrinmIixmr is usab, the memixme fil Nachweis und Zhlung der Sporen sulfitreduzierender Anaerobier (Clostridien); Membranfiltrationsverfahren (IS0 6461 -2 : 1986) DIN EN 26461 part 2 IS0 6461 -2. This standard, together with DIN EN 26461 Part 1, April 1993 edition, super- sedes DI

    4、N 38411 Part 7, November 1986 edition. European Standard EN 26 461 -2 : 1993 has the status of a DIN Standard. A comma is used as the decimal rnarkef This standard is part of the series Deutsche Einheitsverfahren zur Wasser-, Abwasser- und Schlammuntersuchung; mikrobiologische Verfahren (Gruppe K) (

    5、German standard methods for the examination of water, waste water and sludge; microbiological methods (group K). National foreword This standard has been published in accordance with a decision taken by CEN/TC 230 to adopt, without alteration, International Standard IS0 6461 -2 as a European Standar

    6、d. The responsible German body involved in the preparation of this standard was the Normenausschu Wasserivesen (Water Practice Standards Committee). Expert assistance and specialized laboratories will be required to perform the analysis described in this standard. Depending on the objective of the a

    7、nalysis, a check shall be made in each individual case on whether and to what extent additional boundary conditions will have to be specified. NOTE: Standard methods published as DIN Standards are obtainable from Beuth Veriag GmbH, either individ- ually or grouped in volumes. The standard methods in

    8、cluded in the loose-leaf publication entitled Deutsche Einheitsverfahren zur Wasser-, Abwasser- und Schlarnmuntersuchung will continue to be published by VCH Verlagsgesellschaft, Weinheim. The standard methods relating to the Wasserhaushaltsgesetz (German Water Management Act) itself and all the reg

    9、ulations on waste water that have been issued to date are included in DIN-Taschenbuch (DIN Handbook) 230. Standard methods or draft standards bearing the group title German standard methods for the examination of water, waste water and sludge are classified under the following categories (main title

    10、s): General information (group A) (DIN 38402) Physical and physicochemical parameters (group C) (DIN 38404) Anions (group D) (DIN 38405) Cations (group E) (DIN 38406) Substance group analysis (group F) (DIN 38407) Gaseous constituents (group G) (DIN 38408) (DIN 38409) Biological-ecological methods o

    11、f analysis (group M) (DIN 3841 O) Microbiological methods (group K) (DIN 3841 1) Bio-assays (group L) (DIN 38412) Individual constituents (group P) (DIN 38413) Sludge and sediments (group S) (DIN 38414) Bio-assays with microorganisms (group T) (DIN 38515) Information on Parts of these standards that

    12、 have already been published can be obtained from the offices of the Normen- ausschu Wassetwesen, telephone (O 30) 26 O1 -24 23, or from Beufh Ver/ag GmbH, D-10772 Berlin. DIN IS0 3696 is the standard corresponding to International Standard IS0 3696 referred to in clause 3. , Parameters characterizi

    13、ng effects and substances (group H) Continued overleaf. EN comprises 5 pages. Beuth Verlag GmbH, Berlin, has the exclusive right of sale for German Standards (DIN-Normen). 09.93 DIN EN 26 461 Part 2 Engl. Price group 6 Sales No. 1106 Page 2 DIN EN 26 461 Part 2: 1993 Standard referred to (and not in

    14、cluded in References) DIN IS0 3696 Water for laboratory use; requirements and testing Previous edition DIN 38411 Part 7 11.86. Amendments In comparison with DIN 38411 Part 7, November 1986 edition, details of the method (procedure and evaluation ) have been changed. international Patent Classificati

    15、on B O1 D 061 /O0 C12N001/02 C12Q001/06 C 12 R O01 /145 G O1 N 033118 EUROPEAN STANDARD EUROPISCHE NORM NORME EUROPENNE EN 26 461 -2 January 1993 UDC 628.1 /.3 : 620.1 : 543.39 : 579.852.1 3 Descriptors: Water, quality, water analysis, microbiological analysis, microorganisms, sulfite-reducing bacte

    16、ria, clostridium, membrane filtration. English version Water quality Detection and enumeration of the spores of sulfite-reducing anaerobes (clostridia) Method by membrane filtration (IS0 6461 -2: 1986) Qualit de leau; recherche et dnombre- ment de micro-organismes anarobies sulfito-rducteurs (clostr

    17、idia); mthode par filtration sur membrane filtrationsverfahren Wasserbeschaffenheit; Nachweis und Zhlung der Sporen sulfitreduzierender Anaerobier (Clostridien); Membran- (IS0 6461 -2 : 1986) (IS0 6461-2:1986) This European Standard was approved by CEN on 1993-01-20 and is identical to the IS0 Stand

    18、ard as referred to. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national stand- ard without any alteration. Up-to-date lists and bibliographical references concerning such national standard

    19、s may be obtained on application to the Central Secretariat or to any CEN member. This European Standard exists in three official versions (English, French, German). A ver- sion in any other language made by translation under the responsibility of a CEN member into its own language and notified to t

    20、he Central Secretariat has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and United Kingdom. CEN Eu

    21、ropean Committee for Standardization Comit Europen de Normalisation Europisches Komitee fr Normung Central Secretariat: rue de Stassart 36, 8-1050 Brussels O 1993. Copyright reserved to all CEN members. Ref. No. EN 26461-2:1993 E Page 2 EN 26 461 -2 : 1993 Foreword This European Standard is the endo

    22、rsement of International Standard IS0 6461 -2: 1986. Endorsement of IS0 6461 -2 Water quality; detection and enumeration of the spores of sulfite-reducing anaerobes (clostridia); method by membrane filtration was recommended by CEN/Technical Committee 230 ?Water analysis? under whose competence this

    23、 European Standard will henceforth fall. In the countries bound to implement this European Standard, a national standard identical to this European Standard shall be published, and conflicting national standards withdrawn, by July 1993 at the latest In accordance with the CEN/CENELEC Internal Regula

    24、tions, the following countries are bound to implement this European Standard: Austria, Belgium, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and United Kingdom. Endorsement notice The text of the Internation

    25、al Standard IS0 6461 -2: 1986 was approved by CEN as a European Standard without any modifi- cation. Page 3 EN 26 461 -2 : 1993 O Introduction The spores of sulfie-reducing anaerobes (clostridia) are wide- spread in the environment. They are present in human and animal faecal matter, in waste water

    26、and in soil. Unlike Escherichk coli and other coliform organisms, the spores sur- vive in water for long periods as they are more resistant than vegetative forms to the action of chemical and physical factors. They may thus give an indication of remote or intermittent pollution. They may even be res

    27、istant to chlorination at levels which are normally used for the treatment of water, and they are thus useful for control purposes. IS0 6461 consists of the following parts : Part 1 : Method by enrichment in a liquid medium. Part 2: Method by membrane filtration. 1 Scope Ths part of IS0 6461 specifi

    28、es a method for the detection and enumeration of the spores of sulfite-reducing anaerobes (clostridia) by membrane filtration. 2 Field of application The method can be applied to all types of water, except when a large amount of particulate material is liable to be retained by the membrane. 3 Refere

    29、nces IS0 3696. Water for laboratory use - SpecXcations. IS0 5667, Water qualiw - Samphng - Part 2: Guidance on sampling techniques. Part31 Guidance on the preservation and handling of samples. IS0 7W, Water 9udity - Evaluation of membrane filters used for microbiological anaEyses. IS0 8199, Water qu

    30、ality - General guidance for microbiological examination by enumemtion of micro- organisms on culture media. ) 4 Definition For the purpose of this patt of IS0 6461, the following defi- nition applies. clostridia : Sulfite-reducing, spore-forming, anaerobic micro- organisms which belong to the Bacil

    31、laceae family and the genus Clostridium. 5 Principle The detection of spores of sulfite-reducing anaerobes (clostridia) in a specified volume of a water sample requires the following steps. 5.1 Selection of spores Selection of spores in the sample by applying heat for a period of time sufficient to

    32、destroy vegetative bacteria. 5.2 Membrane filtration and culture Filtration of the water sample through a membrane filter having a pore size such that bacterial spores (0,2 pm) are retained in or on the membrane filter. Placing of the filter on a specialized selective culture medium sulfie-iron-agar

    33、), followed by incubation at 37 i: 1 OC for 20 i 4 h and 44 i 4 h. and counting of any black colonies. 6 Culture media and reagents 6.1 Basic materials In order to improve the reproducibility of the results, it is recommended that, for the preparation of the diluents and culture media, dehydrated ba

    34、sic components or complete dehydrated media be used. Similarly, commercially prepared reagents may ab be used. The manufacturers instructions shall be rigorously followed. 1) At present at the stage of draft. Page 4 EN 26461-2:1993 The chemical products used for the preparation of the culture media

    35、and the reagents shall be of recognized analytical quality. The water used shall be distilled or deionized water, free from substances that might inhibit the growth of micro-organisms under the test conditions (see IS0 3696). Measurements of pH shall be made using a pH meter, measurements being refe

    36、rred to a temperature of 25 OC. If the prepared culture media are not used immediately, they shall, unless otherwise stated, be stored in the dark at ap- proximately 4 OC, for no longer than 1 month. 6.2 Suif te-iron-agar 6.2.1 Basal medium (nutrient agar) Composition Meat extract Peptone Sodium chl

    37、oride (NaCl) Agar Water Preparation Steam to dissolve, make up to 1 litre with water, and adjust the pH to 7,6 i 0,l with 1 mol/l sodium hydroxide solution. Sterilize at 121 * 1 OC in the autoclave for 20 min. Store in the refrigerator after solidfiing. 6.2.2 Sodium sulfite (Na$303) solution. Dissol

    38、ve 10 g of sodium sulfite in 100 rnl of water. It is advisable to prepare a fresh solution every two weeks. 6.2.3 Ironill) sulfate (FeSO,) solution. Dissolve 8 g of crystallized iron(l1) sulfate in 100 ml of water. 6.2.4 Complete medium Immediately before use, melt the basal medium (6.2.1) and to ea

    39、ch 18 ml volume add 1 ml of sodium sulfite solution (6.2.2) and five drops of iron(l1) sulfate solution (6.2.3). Add 1 mi of the sodium sulfite solution and 5 drops of the iron(1l) sulfate solution to the agar tubes just before the embed- ding procedure (see 9.3). 6.3. Tryptose-sulfite-agar (alterna

    40、tive medium) Composition Tryptose Soytone Yeast extract Sodium metabisulfite Ammonium iron(ll1) citrate Water Preparation Steam to dissolve, and adjust the pH to 7,6 f 0.1 at 25 OC. Distribute into test tubes in volumes of 18 mi. Sterilize the medium for 15 min at 121 i 1 OC. Store in the refigerato

    41、r at 4 to 5 OC. Discard unused medium 2 weeks after preparation. 7 Apparatus and glassware Usual microbiological laboratory equipment, and 7.1 conical flask), of capacity 2 litres. Glass flasks (Erlenmeyer flask, round-bottom flask, or 7.2 Test tubes, 160 mm x 16 mm. 7.3 divisions of 0,l mi. Graduat

    42、ed pipettes, of capacity 10 ml, graduated in 7.4 Volumetric pipettes, of capacity 10 ml. 7.5 Jars. of capacity 1 litre. 7.6 Steamer. 7.7 Water bath. 7.8 Membrane filtration apparatus. 7.9 Sterile membrane filters, of nominal pore size 0,2 prn. NOTE - The quality of membrane filters may vary accordin

    43、g to brand or even from batch to batch. It is therefore advisable to check the qual- ity on a reguiar basis according to IS0 7704. 7.10 Incubator, capable of being maintained at 37 t 1 C. 7.11 Petri dishes. 8 Sampling Refer to IS0 5667/2 and IS0 8199 for sampling techniques. 9 Procedure 9.1 Treatmen

    44、t of samples Refer to IS0 566713 for guidance on the preservation and handling of samples, and to IS0 8199. 9.2 Selection of spores (technique) Before the test, the sample of water should be heated in a water bath at 75 + 5 OC for 15 min from the time it reaches that temperature. A similar bottle co

    45、ntaining the same vOlUme Page 5 EN 26 461 - 2 : 1993 of water as the test sample should be used periodically as a control in order to check the heating time required. The temperature of the water in the control bottle can be constantly recorded by thermometer. 9.3 Inoculation and incubation Refer to

    46、 IS0 7704 for a general description of the membrane filtration technique. According to the instructions in that document, filter a suitable volume of water. For drinking water, spring and well water, mineral waters, sea-water, and surface water, less polluted with clostridia, filter 100 ml: for high

    47、ly polluted water or sewage use smaller volumes. Volumes of water smaller than 10 ml should be mixed with 10 to 100 ml of sterile water or diluent. Adjust the dilutions so that any resultant black colonies are well separated and can be easily counted. After filtration, remove the membrane with steri

    48、le forceps and place it face downwards on the bottom of a Petri dish, ensuring that no air bubbles are trapped under the filter. Then carefully pour 18 ml of the liquefied complete culture medium, previous- ly cooled to about 50 OC, over the membrane while holding it still with sterile forceps. Afte

    49、r this layer of medium has set, in- cubate anaerobically or under other conditions which ensure anaerobiosis at a temperature of 37 f 1 OC for 20 4 h and 44 f 4 h. If an anaerobic jar or an anaerobic incubator is used, the membrane filter may be placed on the surface of the agar face upwards. 9.4 Interpretation Count all black colonies after incubation for 20 f 4 h and 44 I4h. 10 Expression of results Express the results in accordance with IS0 8199. 11 Test report The test report shall state the method used and express the results as the number of sulf


    注意事项

    本文(DIN EN 26461-2-1993 Water quality detection and enumeration of the spores of sulfite-reducing anaerobes (clostridia) part 2 method by membrane filtration (ISO 6461-2 1986) german v.pdf)为本站会员(medalangle361)主动上传,麦多课文档分享仅提供信息存储空间,仅对用户上传内容的表现方式做保护处理,对上载内容本身不做任何修改或编辑。 若此文所含内容侵犯了您的版权或隐私,请立即通知麦多课文档分享(点击联系客服),我们立即给予删除!




    关于我们 - 网站声明 - 网站地图 - 资源地图 - 友情链接 - 网站客服 - 联系我们

    copyright@ 2008-2019 麦多课文库(www.mydoc123.com)网站版权所有
    备案/许可证编号:苏ICP备17064731号-1 

    收起
    展开