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    BS ISO 4718-2004 Oil of lemongrass [Cymbopogon flexuosus (Nees ex Steudel) J F Watson]《柠檬草[Cymbopogon flexuosus (Nees ex Steudel) J F Watson]油》.pdf

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    BS ISO 4718-2004 Oil of lemongrass [Cymbopogon flexuosus (Nees ex Steudel) J F Watson]《柠檬草[Cymbopogon flexuosus (Nees ex Steudel) J F Watson]油》.pdf

    1、BRITISH STANDARD BS ISO 4718:2004 Oil of lemongrass Cymbopogon flexuosus (Nees ex Steudel) J.F. Watson ICS 71.100.60 BS ISO 4718:2004 This British Standard was published under the authority of the Standards Policy and Strategy Committee on 2 June 2004 BSI 2 June 2004 ISBN 0 580 43841 4 National fore

    2、word This British Standard reproduces verbatim ISO 4718:2004 and implements it as the UK national standard. The UK participation in its preparation was entrusted to Technical Committee AW/54, Essential oils, which has the responsibility to: A list of organizations represented on this committee can b

    3、e obtained on request to its secretary. Cross-references The British Standards which implement international publications referred to in this document may be found in the BSI Catalogue under the section entitled “International Standards Correspondence Index”, or by using the “Search” facility of the

    4、 BSI Electronic Catalogue or of British Standards Online. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. aid enqu

    5、irers to understand the text; present to the responsible international/European committee any enquiries on the interpretation, or proposals for change, and keep the UK interests informed; monitor related international and European developments and promulgate them in the UK. Summary of pages This doc

    6、ument comprises a front cover, an inside front cover, the ISO title page, pages ii and iii, a blank page, pages 1 to 6, an inside back cover and a back cover. The BSI copyright notice displayed in this document indicates when the document was last issued. Amendments issued since publication Amd. No.

    7、 Date Comments Reference number ISO 4718:2004(E)INTERNATIONAL STANDARD ISO 4718 Second edition 2004-05-15 Oil of lemongrass Cymbopogon flexuosus (Nees ex Steudel) J.F. Watson Huile essentielle de lemongrass Cymbopogon flexuosus (Nees ex Steudel) J.F. Watson BSISO4718:2004DPlcsid Fremia ihTs PDF file

    8、 mya ctnoian emdebt dedyfepcaes. In ccacnadrow eitA hebods licnesilop gnic,y tihs file mirp eb yatnde iv roweb detu slahl ton ide ebtlnu deess teh typfecaes wihce era hml era deddebicsnede ti dna onstlalde t noeh comuptfrep reromign tide ehtin.g In wodlnidaot gnihs fil,e trapise atpecc tiereht nser

    9、ehnopsiiblity fo nto ifnriigngn Aebods licnesilop gnic.y ehT ISO tneClar Secrteirata caceptl on siibality in this .aera Ai ebods a tedarmfo kra Aebod SystemI snctaropro.de teDials fo teh sfotwcudorp erats sut deo crtaee tihs PDF file cna f ebi dnuon tlareneG eh Ifnler oatit evt oeh file; tP ehDc-Frt

    10、aeino marapterew stpo ereimizde fro irptni.gn Evyre cera neeb sah takne tsne oeru taht teh file is siutlbae fosu re yb ISO memdob rebeis. In tlnu ehikletneve y ttah lborp aem lertait gno it is f,dnuo plsaee ifnrom ttneC ehlar Secterirata ta teh serddaig sleb nevwo. ii BSISO4718:2004 iiiForeword ISO

    11、(the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical comm

    12、ittee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrote

    13、chnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the memb

    14、er bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifyi

    15、ng any or all such patent rights. ISO 4718 was prepared by Technical Committee ISO/TC 54, Essential oils. This second edition cancels and replaces the first edition (ISO 4718:1981), which has been technically revised. BSISO4718:2004blank BSISO4718:2004INTENRATIONAL TSANDADR IS:8174 O4002(E)1Oil of l

    16、emongrass Cymbopogon flexuosus (Nees ex Steudel) J.F. Watson 1 Scope This International Standard specifies certain charac- teristics of the oil of lemongrass Cymbopogon flexuosus (Nees ex Steudel) J.F. Watson, in order to facilitate assessment of its quality. 2 Normative references The following ref

    17、erenced documents are indispen- sable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO/TR 210, Essential oils General rules for packaging, conditio

    18、ning and storage ISO/TR 211, Essential oils General rules for labelling and marking of containers ISO 212, Essential oils Sampling ISO 279, Essential oils Determination of relative density at 20 C Reference method ISO 280, Essential oils Determination of refractive index ISO 592, Essential oils Dete

    19、rmination of optical rotation ISO 875, Essential oils Evaluation of miscibility in ethanol ISO 11024-1, Essential oils General guidance on chromatographic profiles Part 1: Preparation of chromatographic profiles for presentation in standards ISO 11024-2, Essential oils General guidance on chromatogr

    20、aphic profiles Part 2: Utilization of chromatographic profiles of samples of essential oils 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 oil of lemongrass essential oil obtained by steam distillation of the fresh aerial parts of Cymbopogon

    21、 flexuosus (Nees ex Steudel) J.F. Watson, of the Poaceae family. NOTE For information on the CAS number, see ISO/TR 21092. 4 Requirements 4.1 Appearance Clear, mobile liquid. 4.2 Colour Pale yellow to yellowish brown. 4.3 Odour Strong, green, resembling that of citral. 4.4 Relative density at 20 C,

    22、20 20 d Minimum: 0,885 Maximum: 0,905 4.5 Refractive index at 20 C Minimum: 1,483 Maximum: 1,489 4.6 Optical rotation at 20 C Between 4 and +1. BSISO4718:20042 4.7 Miscibility in ethanol, 70 % (volume fraction), at 20 C It shall not be necessary to use more than 3 volumes of ethanol, 70 % (volume fr

    23、action), to obtain a clear solution with 1 volume of essential oil. 4.8 Chromatographic profile Analysis of the essential oil shall be carried out by gas chromatography. In the chromatogram obtained, the representative and characteristics components shown in Table 1 shall be identified. The proporti

    24、ons of these components, indicated by the integrator, shall be as shown in Table 1. This constitutes the chromatographic profile of the essential oil. Table 1 Chromatographic profile Component Minimum % Maximum % Limonene 0,5 3,5 6-Methyl-5-heptene-2-one 0,1 2,0 Caryophyllene 0,2 3,5 Neral 25,0 35,0

    25、 Geranial 35,0 47,0 Geranyl acetate 0,5 6,0 Geraniol 1,5 8,0 NOTE The chromatographic profile is normative, contrary to typical chromatogram given for information in Annex A. 4.9 Flashpoint Information on the flashpoint is given in Annex B. 5 Sampling See ISO 212. Minimum volume of test sample: 25 m

    26、l. NOTE This volume allows each of the tests specified in this International Standard to be carried out at least once. 6 Test methods 6.1 Relative density at 20 C, 20 20 d See ISO 279. 6.2 Refractive index at 20 C See ISO 280. 6.3 Optical rotation at 20 C See ISO 592. 6.4 Miscibility in ethanol, 70

    27、% (volume fraction), at 20 C See ISO 875. 6.5 Chromatographic profile See ISO 11024-1 and ISO 11024-2. 7 Packaging, labelling, marking and storage See ISO/TR 210 and ISO/TR 211. BSISO4718:2004 3Annex A (informative) Typical chromatograms of the analysis by gas chromatography of the essential oil of

    28、lemongrass Cymbopogon flexuosus (Nees ex Steudel) J.F. Watson Peak identification Operating conditions 1 6-Methyl-5-hepten-2-one 2 Limonene 3 Neral 4 Geraniol 5 Geranial 6 Geranyl acetate 7 Caryophyllene Column: capillary; length 30 m; internal diameter 320 m Stationary phase: poly(dimethyl siloxane

    29、) Film thickness: 0,25 m Oven temperature: isothermal at 70 C for 10 min, then temperature programming from 70 C to 220 C at a rate of 2 C/min Injector temperature: 250 C Detector temperature: 250 C Detector: flame ionization type Carrier gas: hydrogen Volume injected: 0,2 l Carrier gas flow rate: 0

    30、,3 ml/min Figure A.1 Typical chromatogram taken on an apolar column BSISO4718:20044 Peak identification Operating conditions 1 Limonene 2 6-Methyl-5-hepten-2-one 3 Citronellal 4 Caryophyllene 5 Neral 6 Geranial 7 Geranyl acetate 8 Geraniol Column: capillary; length 10 m; internal diameter 0,100 mm S

    31、tationary phase: poly(ethylene glycol) (Supercowax-10) Film thickness: 0,1 m Oven temperature: 60 C to 240 C at a rate of 12 C/min, then 240 C for 18 min Injector temperature: 250 C Detector temperature: 250 C Detector: flame ionization type Carrier gas: hydrogen Volume injected: 1 l Carrier gas flo

    32、w rate: 0,7 ml/min Split ratio: 150/1 Figure A.2 Typical chromatogram taken on a polar column BSISO4718:2004 5Annex B (informative) Flashpoint B.1 General information For safety reasons, transport companies, insurance companies, and people in charge of safety services require information on the flas

    33、hpoints of essential oils, which in most cases are flammable products. A comparative study on the relevant methods of analysis (see ISO/TR 11018) concluded that it was difficult to recommend a single apparatus for standardization purposes, given that: there is a wide variation in the chemical com- p

    34、osition of essential oils; the volume of the sample needed in certain requirements would be too costly for high- priced essential oils; as there are several different types of equip- ment which can be used for the determination, users cannot be expected to use one specified type only. Consequently,

    35、it was decided to give a mean value for the flashpoint in an informative annex to each International Standard, in order to meet the requirements of the interested parties. The equipment with which this value was obtained should be specified. For further information, see ISO/TR 11018. B.2 Flashpoint

    36、of the essential oil of lemongrass The mean value is +89 C. NOTE Obtained with Pensky Martens equipment. BSISO4718:20046 Bibliography 1 ISO/TR 11018:1997, Essential oils General guidance on the determination of flashpoint 2 ISO/TR 21092: 1) , Essential oils Characterization 1) To be published. BSISO

    37、4718:2004blankBS ISO 4718:2004 BSI 389 Chiswick High Road London W4 4AL BSI British Standards Institution BSI is the independent national body responsible for preparing British Standards. It presents the UK view on standards in Europe and at the international level. It is incorporated by Royal Chart

    38、er. Revisions British Standards are updated by amendment or revision. Users of British Standards should make sure that they possess the latest amendments or editions. It is the constant aim of BSI to improve the quality of our products and services. We would be grateful if anyone finding an inaccura

    39、cy or ambiguity while using this British Standard would inform the Secretary of the technical committee responsible, the identity of which can be found on the inside front cover. Tel: +44 (0)20 8996 9000. Fax: +44 (0)20 8996 7400. BSI offers members an individual updating service called PLUS which e

    40、nsures that subscribers automatically receive the latest editions of standards. Buying standards Orders for all BSI, international and foreign standards publications should be addressed to Customer Services. Tel: +44 (0)20 8996 9001. Fax: +44 (0)20 8996 7001. Email: ordersbsi-. Standards are also av

    41、ailable from the BSI website at http:/www.bsi-. In response to orders for international standards, it is BSI policy to supply the BSI implementation of those that have been published as British Standards, unless otherwise requested. Information on standards BSI provides a wide range of information o

    42、n national, European and international standards through its Library and its Technical Help to Exporters Service. Various BSI electronic information services are also available which give details on all its products and services. Contact the Information Centre. Tel: +44 (0)20 8996 7111. Fax: +44 (0)

    43、20 8996 7048. Email: infobsi-. Subscribing members of BSI are kept up to date with standards developments and receive substantial discounts on the purchase price of standards. For details of these and other benefits contact Membership Administration. Tel: +44 (0)20 8996 7002. Fax: +44 (0)20 8996 700

    44、1. Email: membershipbsi-. Information regarding online access to British Standards via British Standards Online can be found at http:/www.bsi- Further information about BSI is available on the BSI website at http:/www.bsi-. Copyright Copyright subsists in all BSI publications. BSI also holds the cop

    45、yright, in the UK, of the publications of the international standardization bodies. Except as permitted under the Copyright, Designs and Patents Act 1988 no extract may be reproduced, stored in a retrieval system or transmitted in any form or by any means electronic, photocopying, recording or other

    46、wise without prior written permission from BSI. This does not preclude the free use, in the course of implementing the standard, of necessary details such as symbols, and size, type or grade designations. If these details are to be used for any other purpose than implementation then the prior written permission of BSI must be obtained. Details and advice can be obtained from the Copyright & Licensing Manager. Tel: +44 (0)20 8996 7070. Fax: +44 (0)20 8996 7553. Email: copyrightbsi-.


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