1、BSI Standards PublicationBS ISO 4716:2013Essential oil of vetiver Chrysopogon zizanioides(L.) Roberty, syn. V e t i v e r i a zizanioides (L.) NashBS ISO 4716:2013 BRITISH STANDARDNational forewordThis British Standard is the UK implementation of ISO 4716:2013.The UK participation in its preparation
2、 was entrusted to TechnicalCommittee AW/54, Essential oils.A list of organizations represented on this committee can beobtained on request to its secretary.This publication does not purport to include all the necessaryprovisions of a contract. Users are responsible for its correctapplication. The Br
3、itish Standards Institution 2013. Published by BSI StandardsLimited 2013ISBN 978 0 580 73631 5ICS 71.100.60Compliance with a British Standard cannot confer immunity fromlegal obligations.This British Standard was published under the authority of theStandards Policy and Strategy Committee on 31 Augus
4、t 2013.Amendments issued since publicationDate Text affectedBS ISO 4716:2013 ISO 2013Essential oil of vetiver Chrysopogon zizanioides (L.) Roberty, syn. Vetiveria zizanioides (L.) NashHuile essentielle de vtiver Chrysopogon zizanioides (L.) Roberty, syn. Vetiveria zizanioides (L.) NashINTERNATIONAL
5、STANDARDISO4716Third edition2013-08-01Reference numberISO 4716:2013(E)BS ISO 4716:2013ISO 4716:2013(E)ii ISO 2013 All rights reservedCOPYRIGHT PROTECTED DOCUMENT ISO 2013All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form o
6、r by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISOs member body in the country of the requester.ISO copyright officeCase postale 56 CH-1
7、211 Geneva 20Tel. + 41 22 749 01 11Fax + 41 22 749 09 47E-mail copyrightiso.orgWeb www.iso.orgPublished in SwitzerlandBS ISO 4716:2013ISO 4716:2013(E) ISO 2013 All rights reserved iiiContents PageForeword iv1 Scope . 12 Normative references 13 Terms and definitions . 14 Requirements 14.1 Appearance
8、14.2 Colour 14.3 Odour . 24.4 Relative density at 20 C, d202024.5 Refractive index at 20 C . 24.6 Optical rotation at 20 C 24.7 Miscibility in ethanol 80 % volume fraction at 20 C 24.8 Acid value. 24.9 Chromatographic profile 24.10 Flashpoint 35 Sampling 36 Test methods . 36.1 Relative density at 20
9、 C, d202036.2 Refractive index at 20 C . 36.3 Optical rotation at 20 C 36.4 Miscibility in ethanol 80 % volume fraction at 20 C 36.5 Acid value. 36.6 Chromatographic profile 37 Packaging, labelling, marking and storage . 4Annex A (informative) Typical chromatograms of the analysis by gas chromatogra
10、phy of the essential oil of vetiver Chrysopogon zizanioides (L.) Roberty, syn. Vetiveria zizanioides (L.) Nash . 5Annex B (informative) Flashpoint 10Bibliography .11BS ISO 4716:2013ISO 4716:2013(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of national
11、standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International
12、 organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.The procedures used to develop this document and those intended for
13、 its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the different types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2, www.iso.org/dir
14、ectives.Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the document will be in the
15、 Introduction and/or on the ISO list of patent declarations received, www.iso.org/patents.Any trade name used in this document is information given for the convenience of users and does not constitute an endorsement.The committee responsible for this document is ISO/TC 54, Essential oils.This third
16、edition cancels and replaces the second edition (ISO 4716:2002), which has been technically revised.iv ISO 2013 All rights reservedBS ISO 4716:2013INTERNATIONAL STANDARD ISO 4716:2013(E)Essential oil of vetiver Chrysopogon zizanioides (L.) Roberty, syn. Vetiveria zizanioides (L.) Nash1 ScopeThis Int
17、ernational Standard specifies certain characteristics of the essential oil of vetiver Chrysopogon zizanioides (L.) Roberty, syn. Vetiveria zizanioides (L.) Nash, growing in Reunion Island, China, Haiti, Indonesia, Madagascar, Brazil, and Comoros Island in order to facilitate assessment of its qualit
18、y.2 Normative referencesThe following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amen
19、dments) applies.ISO/TR 210, Essential oils General rules for packaging, conditioning and storageISO/TR 211, Essential oils General rules for labelling and marking of containersISO 212, Essential oils SamplingISO 279, Essential oils Determination of relative density at 20 C Reference methodISO 280, E
20、ssential oils Determination of refractive indexISO 592, Essential oils Determination of optical rotationISO 875, Essential oils Evaluation of miscibility in ethanolISO 1242, Essential oils Determination of acid valueISO 11024 (all parts), Essential oils General guidance on chromatographic profiles3
21、Terms and definitionsFor the purposes of this document, the following terms and definitions apply.3.1essential oil of vetiveressential oil obtained by steam distillation of the roots of Chrysopogon zizanioides (L.) Roberty, syn. Vetiveria zizanioides (L.) Nash of the Poaceae familyNote 1 to entry: F
22、or information on the CAS number, see ISO/TR 21092.24 Requirements4.1 AppearanceViscous liquid.4.2 ColourFrom yellowish brown to reddish brown. ISO 2013 All rights reserved 1BS ISO 4716:2013ISO 4716:2013(E)4.3 OdourCharacteristic, woody and earthy.4.4 Relative density at 20 C, d2020Value Bourbon typ
23、eaChina Haiti Indonesia BrazilMinimum 0,990 0,985 0,980 0,980 0,990Maximum 1,015 1,020 1,005 1,003 1,010aBourbon type includes: Reunion Island, Comoros Island and Madagascar origins.4.5 Refractive index at 20 CValue Bourbon typeaChina Haiti Indonesia BrazilMinimum 1,522 1,520 1,516 1,520 1,520Maximu
24、m 1,530 1,528 1,527 1,530 1,530aBourbon type includes: Reunion Island, Comoros Island and Madagascar origins.4.6 Optical rotation at 20 CValue Bourbon typeaChina Haiti Indonesia BrazilMinimum +19 +17 +22 +17 +15Maximum +30 +46 +48 +32 +30aBourbon type includes: Reunion Island, Comoros Island and Mad
25、agascar origins.4.7 Miscibility in ethanol 80 % volume fraction at 20 CIt shall not be necessary to use more than 2 volumes of ethanol 80 % volume fraction to obtain a clear solution with 1 volume of essential oil.NOTE A slight opalescence can sometimes be observed.4.8 Acid valueValue Bourbon typeaC
26、hina Haiti Indonesia BrazilMinimum 4,5 10 1 10 30Maximum 35 70 6 35 60aBourbon type includes: Reunion Island, Comoros Island and Madagascar origins.4.9 Chromatographic profileCarry out the analysis of the essential oil by gas chromatography. Identify in the chromatogram obtained the representative a
27、nd characteristic components shown in Table 1. The proportions of these components, indicated by the integrator, shall be as shown in Table 1. This constitutes the chromatographic profile of the essential oil.Important Due to the viscosity of this essential oil, it is necessary to dilute it with 1,8
28、-cineole so that the final mass fraction of each component in the mixture is 50 %. It is also recommended to use a polyethylene glycol type polar column. There will be two integrations, with and without 1,8-cineole.2 ISO 2013 All rights reservedBS ISO 4716:2013ISO 4716:2013(E)Table 1 Chromatographic
29、 profileComponentBourbon typeaChina Haiti Indonesia Brazilmin. %max. %min. %max. %min. %max. %min. %max. %min. %max. %-Vetivenene 3 6 2 8 0,7 3 4 9 6 9-Vetivone 2 5 2 4 2 4 2 4 2 4Khusimol 12 18 5 15 9 15 6 11 8 13-Vetivone 3 6 2 5 2 4 2 4 1 4Isovalencenol 6 14 1 11 10 16 1 7 5 11The above percentag
30、es are obtained without integration of 1,8-cineole.1,8-Cineole 53 53 53 53 53NOTE The chromatographic profile is normative, contrary to typical chromatograms given for information in Annex A.aBourbon type includes: Reunion Island, Comoros Island, and Madagascar origins.4.10 FlashpointInformation on
31、the flashpoint is given in Annex B.5 SamplingSampling shall be performed in accordance with ISO 212.Minimum volume of test sample: 25 ml.NOTE This volume allows each of the tests specified in this International Standard to be carried out at least once.6 Test methods6.1 Relative density at 20 C, d202
32、0Determine the relative density in accordance with ISO 279.6.2 Refractive index at 20 CDetermine the refractive index in accordance with ISO 280.6.3 Optical rotation at 20 CDetermine the optical rotation in accordance with ISO 592 on a 10 % solution in ethanol 95 % volume fraction.6.4 Miscibility in
33、 ethanol 80 % volume fraction at 20 CDetermine the miscibility in accordance with ISO 875.6.5 Acid valueDetermine the acid value in accordance with ISO 1242.6.6 Chromatographic profileDetermine the chromatographic profile in accordance with ISO 11024. ISO 2013 All rights reserved 3BS ISO 4716:2013IS
34、O 4716:2013(E)7 Packaging, labelling, marking and storageThese items shall be in accordance with ISO/TR 210 and ISO/TR 211.4 ISO 2013 All rights reservedBS ISO 4716:2013ISO 4716:2013(E)Annex A (informative) Typical chromatograms of the analysis by gas chromatography of the essential oil of vetiver C
35、hrysopogon zizanioides (L.) Roberty, syn. Vetiveria zizanioides (L.) Nash ISO 2013 All rights reserved 5BS ISO 4716:2013ISO 4716:2013(E)A.1 Essential oil of vetiver, HaitiFigure A.1 is a typical chromatogram of the analysis of the essential oil of vetiver, Haiti, diluted with 1,8-cineole so that the
36、 final mass fraction of each component in the mixture is 50 %.Peak identification Operating conditions1 -Vetivenene Column: capillary; 50 m long; 0,2 mm internal diameter2 -Vetivone Stationary phase: polyethylene glycol 20Ma3 Khusimol Film thickness: 0,25 m4 -Vetivone Oven temperature: programming f
37、rom 65 C to 230 C, at a rate of 2 C/min5 Isovalencenol Injector temperature: 250 C6 Zizanoic acid Detector temperature: 250 CDetector: flame ionization typeCarrier gas: HydrogenVolume injected: 0,2 lt time Carrier gas flow rate: 1,1 ml/minSplit ratio: 1/100aProduct available commercially. This infor
38、mation is given for the convenience of users of this document and does not constitute an endorsement by ISO of this product.Figure A.1 Typical chromatogram taken on a polar column6 ISO 2013 All rights reservedBS ISO 4716:2013ISO 4716:2013(E)A.2 Essential oil of vetiver, IndonesiaFigure A.2 is a typi
39、cal chromatogram of the analysis of the essential oil of vetiver, Indonesia, diluted with 1,8-cineole so that the final mass fraction of each component in the mixture is 50 %.Peak identification Operating conditions1 -Vetivenene Column: capillary; 50 m long; 0,2 mm internal diameter2 -Vetivone Stati
40、onary phase: polyethylene glycol 20Ma3 Khusimol Film thickness: 0,25 m4 -Vetivone Oven temperature: programming from 65 C to 230 C, at a rate of 2 C/min5 Isovalencenol Injector temperature: 250 C6 Zizanoic acid Detector temperature: 250 CDetector: flame ionization typeCarrier gas: HydrogenVolume inj
41、ected: 0,2 lt time Carrier gas flow rate: 1,1 ml/minSplit ratio: 1/100aProduct available commercially. This information is given for the convenience of users of this document and does not constitute an endorsement by ISO of this product.Figure A.2 Typical chromatogram taken on a polar column ISO 201
42、3 All rights reserved 7BS ISO 4716:2013ISO 4716:2013(E)A.3 Essential oil of vetiver, BrazilFigure A.3 is a typical chromatogram of the analysis of the essential oil of vetiver, Brazil, diluted with 1,8-cineole so that the final mass fraction of each component in the mixture is 50 %.Peak identificati
43、on Operating conditions1 -Vetivenene Column: capillary; 30 m long; 0,32 mm internal diameter2 -Vetivone Stationary phase: polyethylene glycol3 Khusimol Film thickness: 0,25 m4 -Vetivone Oven temperature: programming from 65 C to 210 C, at a rate of 2 C/min5 Isovalencenol Injector temperature: 250 C6
44、 Zizanoic acid Detector temperature: 250 CDetector: flame ionization typeCarrier gas: HeliumVolume injected: 0,2 lt time Carrier gas flow rate: 3,3 ml/minSplit ratio: 1/50Figure A.3 Typical chromatogram taken on a polar column8 ISO 2013 All rights reservedBS ISO 4716:2013ISO 4716:2013(E)A.4 Essentia
45、l oil of vetiver, ChinaFigure A.4 is a typical chromatogram of the analysis of the essential oil of vetiver, China, diluted with 1,8-cineole so that the final mass fraction of each component in the mixture is 50 %.Peak identification Operating conditions1 -Vetivenene Column: capillary; 60 m long; 0,
46、25 mm internal diameter2 -Vetivone Stationary phase: polyethylene glycol3 Khusimol Film thickness: 0,25 m4 -Vetivone Oven temperature: programming from 65 C to 230 C, at a rate of 2 C/min5 Isovalencenol Injector temperature: 250 C6 Zizanoic acid Detector temperature: 250 CDetector: flame ionization
47、typeCarrier gas: HeliumVolume infected: 0,2 lt time Carrier gas flow rate: 1,0 ml/minSplit ratio: 1/100Figure A.4 Typical chromatogram taken on a polar column ISO 2013 All rights reserved 9BS ISO 4716:2013ISO 4716:2013(E)Annex B (informative) FlashpointB.1 General informationFor safety reasons, tran
48、sport companies, insurance companies, and people in charge of safety services require information on the flashpoints of essential oils, which in most cases are flammable products.A comparative study on the relevant methods of analysis (see ISO/TR 110181) concluded that it was difficult to recommend
49、a single apparatus for standardization purposes, given that: there is a wide variation in the chemical composition of essential oils; the volume of the sample needed in certain requirements would be too costly for high-priced essential oils; as there are several different types of equipment which can be used for the determination, users cannot be expected to use one specified type only.Consequently, it was decided to give a
