1、BS ISO 3848:2016Essential oil of citronella, JavatypeBSI Standards PublicationWB11885_BSI_StandardCovs_2013_AW.indd 1 15/05/2013 15:06BS ISO 3848:2016 BRITISH STANDARDNational forewordThis British Standard is the UK implementation of ISO 3848:2016.The UK participation in its preparation was entruste
2、d to Technical Committee AW/54, Essential oils.A list of organizations represented on this committee can be obtained on request to its secretary.This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. The British Sta
3、ndards Institution 2016.Published by BSI Standards Limited 2016ISBN 978 0 580 86049 2 ICS 71.100.60 Compliance with a British Standard cannot confer immunity from legal obligations.This British Standard was published under the authority of the Standards Policy and Strategy Committee on 30 November 2
4、016.Amendments/corrigenda issued since publicationDate T e x t a f f e c t e dBS ISO 3848:2016 ISO 2016Essential oil of citronella, Java typeHuile essentielle de citronnelle, type JavaINTERNATIONAL STANDARDISO3848Third edition2016-11-01Reference numberISO 3848:2016(E)BS ISO 3848:2016ISO 3848:2016(E)
5、ii ISO 2016 All rights reservedCOPYRIGHT PROTECTED DOCUMENT ISO 2016, Published in SwitzerlandAll rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting
6、 on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISOs member body in the country of the requester.ISO copyright officeCh. de Blandonnet 8 CP 401CH-1214 Vernier, Geneva, SwitzerlandTel. +41 22 749 01 11Fax +41 22 74
7、9 09 47copyrightiso.orgwww.iso.orgBS ISO 3848:2016ISO 3848:2016(E)Foreword iv1 Scope . 12 Normative references 13 Terms and definitions . 14 Requirements 15 Flashpoint 26 Sampling 37 Packaging, labelling, marking and storage . 3Annex A (informative) Typical chromatograms of the analysis by gas chrom
8、atography of the essential oil of citronella, Java type 4Annex B (informative) Flashpoint . 6Bibliography 7 ISO 2016 All rights reserved iiiContents PageBS ISO 3848:2016ISO 3848:2016(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of national standards bo
9、dies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizatio
10、ns, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.The procedures used to develop this document and those intended for its further
11、 maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the different types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives)
12、.Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the document will be in the Introd
13、uction and/or on the ISO list of patent declarations received (see www.iso.org/patents).Any trade name used in this document is information given for the convenience of users and does not constitute an endorsement.For an explanation on the meaning of ISO specific terms and expressions related to con
14、formity assessment, as well as information about ISOs adherence to the World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see the following URL: www.iso.org/iso/foreword.html.The committee responsible for this document is ISO/TC 54, Essential oils.This third edition c
15、ancels and replaces the second edition (ISO 3848:2001) which has been technically revised. It also incorporates the Technical Corrigendum ISO 3848:2001/Cor.1:2002.iv ISO 2016 All rights reservedBS ISO 3848:2016INTERNATIONAL STANDARD ISO 3848:2016(E)Essential oil of citronella, Java type1 ScopeThis d
16、ocument specifies certain characteristics of the essential oil of citronella, Java type, in order to facilitate assessment of its quality.2 Normative referencesThe following documents are referred to in the text in such a way that some or all of their content constitutes requirements of this documen
17、t. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.ISO/TS 210, Essential oils General rules for packaging, conditioning and storageISO/TS 211, Essential oils General rules for labelling and
18、 marking of containersISO 212, Essential oils SamplingISO 279, Essential oils Determination of relative density at 20 C Reference methodISO 280, Essential oils Determination of refractive indexISO 592, Essential oils Determination of optical rotationISO 875, Essential oils Evaluation of miscibility
19、in ethanolISO 11024 (all parts), Essential oils General guidance on chromatographic profiles3 Terms and definitionsFor the purposes of this document, the following terms and definitions apply.ISO and IEC maintain terminological databases for use in standardization at the following addresses: IEC Ele
20、ctropedia: available at http:/www.electropedia.org/ ISO Online browsing platform: available at http:/www.iso.org/obp3.1essential oil of citronella, Java typeessential oil obtained by steam distillation of the aerial parts, fresh or partially dried, of Cymbopogon winterianus Jowitt, cultivated in Sou
21、th-East Asia, China, India, Indonesia, Central and South AmericaNote 1 to entry: For information on the CAS number, see ISO/TR 21092.4 Requirements4.1 Essential oil of citronella, Java type shall meet the requirements as given in Table 1. ISO 2016 All rights reserved 1BS ISO 3848:2016ISO 3848:2016(E
22、)Table 1 Requirements for the essential oil of citronella, Java typeCharacteristics RequirementsTest methodAppearance Clear, sometimes slightly opalescent, mobile liquidColour Pale yellow to pale yellowish brown Odour Slightly sweet, floral, rosy, lemon-like Relative density at 20 C, d2020 0,880 to
23、0,902 ISO 279Refractive index at 20 C 1,466 3 to 1,477 0 ISO 280Optical rotation at 20 C Between 5 and +1 ISO 592Miscibility in ethanol, 80 % (volume frac-tion), at 20 CIt shall not be necessary to use more than 2 volumes of ethanol, 80 % (volume fraction), to obtain a clear solution with 1 volume o
24、f essential oil.Sometimes opalescence can arise on continu-ing the addition of ethanol.ISO 8754.2 Carry out the analysis of the essential oil by gas chromatography. Determine the chromatographic profile in accordance with ISO 11024 (all parts). Identify in the chromatogram obtained, the representati
25、ve and characteristic components shown in Table 2. The proportions of these components, indicated by the integrator, shall be as shown in Table 2. This constitutes the chromatographic profile of the essential oil.Table 2 Chromatographic profileComponentaMinimum%Maximum%Limonene 2,0 5,0Citronellal 31
26、,0 40,0Linalool 0,5 1,5Isopulegol 0,5 1,7-Elemene 0,7 2,5Citronellyl acetate 2,0 4,0Germacrene-D 1,5 3,0Geranial 0,3 1,0Geranyl acetateb2,5 5,5-Cadineneb1,5 2,5Citronellol 8,5 14,0Geraniol 20,0 25,0Elemol 1,3 4,8Eugenol 0,5 1,0NOTE The chromatographic profile is normative, contrary to typical chroma
27、tograms given for information in Annex A.aComponents are listed according to their elution order on a polar column (see Figure A.2).bArea %: Values based on apolar column data (see Figure A.1).5 FlashpointInformation on the flashpoint is given in Annex B.2 ISO 2016 All rights reservedBS ISO 3848:201
28、6ISO 3848:2016(E)6 SamplingSampling shall be performed in accordance with ISO 212.Minimum volume of test sample: 50 ml.NOTE This volume allows each of the tests specified in this document to be carried out at least once.7 Packaging, labelling, marking and storageThese items shall be in accordance wi
29、th ISO/TS 210 and ISO/TS 211. ISO 2016 All rights reserved 3BS ISO 3848:2016ISO 3848:2016(E)Annex A (informative) Typical chromatograms of the analysis by gas chromatography of the essential oil of citronella, Java typePeak identification Operating conditions1 Limonene Column: capillary, fused silic
30、a; length 60 m; internal diameter 0,32 mm2+4 Citronellal + Isopulegol Film thickness: 0,25 m3 Linalool Stationary phase: polydimethyl siloxane DB-1a5 -Elemene Oven temperature: temperature programming from 80 C to 220 C at a rate of 4 C/min 6 Citronellyl acetate7 Germacrene-D Injector temperature: 2
31、50 C8 Geranial Detector temperature: 280 C9 Geranyl acetate Detector: flame ionization type10 -Cadinene Carrier gas: helium11 Citronellol Volume injected: 0,15 l12 Geraniol Carrier gas flow rate: 4 ml/min13 Elemol Split ratio: 1/4014 Eugenol aDB-1 is an example of a suitable product available commer
32、cially. This information is given for the convenience of users of this document and does not constitute an endorsement by ISO of this product.Figure A.1 Typical chromatogram taken on an apolar column for essential oil of citronella, Java type4 ISO 2016 All rights reservedBS ISO 3848:2016ISO 3848:201
33、6(E)Peak identification Operating conditions1 Limonene Column: capillary, fused silica; length 60 m; internal diameter 0,32 mm2 Citronellal Stationary phase: polyethylene glycol DB-WAXa3 Linalool Film thickness: 0,25 m4 Isopulegol Oven temperature: temperature programming from 80 C to 220 C at a rat
34、e of 4 C/min5 -Elemene6 Citronellyl acetate Injector temperature: 250 C7 Germacrene-D Detector temperature: 280 C8 Geranial Detector: flame ionization type9+10 Geranyl acetate + -cadinene Carrier gas: helium11 Citronellol Volume injected: 0,15 l12 Geraniol Carrier gas flow rate: 4 ml/min13 Elemol Sp
35、lit ratio: 1/4014 Eugenol aDB-WAX is an example of a suitable product available commercially. This information is given for the convenience of users of this document and does not constitute an endorsement by ISO of this product.Figure A.2 Typical chromatogram taken on a polar column for essential oi
36、l of citronella, Java type ISO 2016 All rights reserved 5BS ISO 3848:2016ISO 3848:2016(E)Annex B (informative) FlashpointB.1 General informationFor safety reasons, transport companies, insurance companies, and people in charge of safety services require information on the flash points of essential o
37、ils, which in most cases are flammable products.A comparative study on the relevant methods of analysis (see ISO/TR 11018) concluded that it was difficult to recommend a single apparatus for standardization purposes, given that: there is a wide variation in the chemical composition of essential oils
38、; the volume of the sample needed in certain requirements would be too costly for high priced essential oils; as there are several different types of equipment which can be used for the determination, users cannot be expected to use one specified type only.Consequently, it was decided to give a mean
39、 value for the flashpoint annexed to each International Standard, for information, in order to meet the requirements of the interested parties.The equipment with which this value was obtained has to be specified.For further information, see ISO/TR 11018.B.2 Flashpoint of the essential oil of citrone
40、llaThe mean value is about +81 C.NOTE Obtained with “GRABNER INSTRUMENTS MINIFLASH-FLPL”1)equipment.1) Equipment available commercially. This information is given for the convenience of users of this document and does not constitute an endorsement by ISO of this product.6 ISO 2016 All rights reserve
41、dBS ISO 3848:2016ISO 3848:2016(E)Bibliography1 ISO 3218, Essential oils Principles of nomenclature2 ISO/TR 11018, Essential oils General guidance on the determination of flashpoint3 ISO/TR 21092, Essential oils Characterization ISO 2016 All rights reserved 7BS ISO 3848:2016ISO 3848:2016(E) ISO 2016
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