1、BRITISH STANDARD BS ISO 3033-3:2005 Oil of spearmint Part 3: Indian type (Mentha spicata L.), redistilled oil ICS 71.100.60 BS ISO 3033-3:2005 This British Standard was published under the authority of the Standards Policy and Strategy Committee on 7 November 2005 BSI 7 November 2005 National forewo
2、rd aid enquirers to understand the text; present to the responsible international/European committee any enquiries on the interpretation, or proposals for change, and keep UK interests informed; monitor related international and European developments and promulgate them in the UK. A list of organiza
3、tions represented on this committee can be obtained on request to its secretary. Cross-references The British Standards which implement international publications referred to in this document may be found in the BSI Catalogue under the section entitled “International Standards Correspondence Index”,
4、 or by using the “Search” facility of the BSI Electronic Catalogue or of British Standards Online. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. Compliance with a British Standard does not of itself confer
5、immunity from legal obligations. Summary of pages This document comprises a front cover, an inside front cover, the ISO title page, pages ii and iii, a blank page, pages 1 to 7 and a back cover. The BSI copyright notice displayed in this document indicates when the document was last issued. Amendmen
6、ts issued since publication Amd. No. Date Comments This British Standard reproduces verbatim ISO 3033-3:2005 and implements it as the UK national standard. The UK participation in its preparation was entrusted to Technical Committee AW/54, Essential oils, which has the responsibility to: ISBN 0 580
7、46748 1 Reference number ISO 3033-3:2005(E)INTERNATIONAL STANDARD ISO 3033-3 First edition 2005-09-01 Oil of spearmint Part 3: Indian type (Mentha spicata L.), redistilled oil Huile essentielle de menthe crpue (ou menthe verte) Partie 3: Type Inde (Mentha spicata L.), huile bidistille BS ISO 3033-3:
8、2005ii iii Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject fo
9、r which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on
10、all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees ar
11、e circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held r
12、esponsible for identifying any or all such patent rights. ISO 3033-3 was prepared by Technical Committee ISO/TC 54, Essential oils. This first edition of ISO 3033-3, together with ISO 3033-1:2005, ISO 3033-2:2005 and ISO 3033-4:2005, cancels and replaces ISO 3033:1988, which has been technically rev
13、ised. ISO 3033 consists of the following parts, under the general title Oil of spearmint: Part 1: Native type (Mentha spicata L.) Part 2: Chinese type (80 % and 60 %) (Mentha viridis L. var. crispa Benth.), redistilled oil Part 3: Indian type (Mentha spicata L.), redistilled oil Part 4: Scotch varie
14、ty (Mentha gracilis Sole) BS ISO 3033-3:2005blank1 Oil of spearmint Part 3: Indian type (Mentha spicata L.), redistilled oil 1 Scope This part of ISO 3033 specifies certain characteristics of the oil of spearmint, Indian type (Mentha spicata L.), redistilled oil, in order to facilitate assessment of
15、 its quality. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO/TR 210,
16、Essential oils General rules for packaging, conditioning and storage ISO/TR 211, Essential oils General rules for labelling and marking of containers ISO 212, Essential oils Sampling ISO 279, Essential oils Determination of relative density at 20 C Reference method ISO 280, Essential oils Determinat
17、ion of refractive index ISO 592, Essential oils Determination of optical rotation ISO 875, Essential oils Evaluation of miscibility in ethanol ISO 1271, Essential oils Determination of carbonyl value Free hydroxylamine method ISO 11024-1, Essential oils General guidance on chromatographic profiles P
18、art 1: Preparation of chromatographic profiles for presentation in standards ISO 11024-2, Essential oils General guidance on chromatographic profiles Part 2: Utilization of chromatographic profiles of samples of essential oils 3 Terms and definitions For the purposes of this document, the following
19、terms and definitions apply. 3.1 oil of spearmint, Indian type, redistilled essential oil obtained by steam distillation from the fresh above-ground parts of the flowering plant of Mentha spicata L. NOTE 1 The steam-distilled oil obtained is redistilled to yield a carvone content of 60 % minimum. NO
20、TE 2 For information on the CAS number, see ISO/TR 21092. 4 Requirements 4.1 Appearance Clear mobile liquid. 4.2 Colour From colourless to pale yellow. 4.3 Odour Characteristic odour of carvone with an herbaceous note. 4.4 Relative density at 20 C, 20 20 d Minimum: 0,921 0 Maximum: 0,938 0 4.5 Refra
21、ctive index at 20 C Minimum: 1,484 0 Maximum: 1,491 0 BS ISO 3033-3:2005BS ISO 3033-3:2005 2 4.6 Optical rotation at 20 C Between 59 and 48. 4.7 Miscibility in ethanol, 70 % (volume fraction), at 20 C It shall not be necessary to use more than 3 volumes of ethanol, 70 % (volume fraction), to obtain
22、a clear solution with 1 volume of essential oil. 4.8 Carbonyl value Minimum: 224, corresponding to a carbonyl compound content of 60 %, expressed as carvone. 4.9 Chromatographic profile Analysis of the essential oil shall be carried out by gas chromatography. In the chromatogram obtained, the repres
23、entative and characteristic components shown in Table 1 shall be identified. The proportions of these components, indicated by the integrator, shall be as shown in Table 1. This constitutes the chromato- graphic profile of the essential oil. Table 1 Chromatographic profile Component Minimum % Maximu
24、m % Limonenea11,5 16,5 3-Octanol 0,6 1,4 Menthoneb 0,15 trans-Sabinene hydrate 0,5 1,0 cis-Dihydrocarvone 1,0 2,5 Carvonec60,0 70,0 trans-Dihydrocarvyl acetate 0,1 0,6 cis-Carvyl acetate 0,1 0,6 cis-Jasmone 0,1 0,4 -Bourbonene 1,0 2,0 Viridiflorol not detectable a The limonene found is regarded to b
25、e predominantly L-limonene based on the physical testing. It is believed that there might be a small amount of D-limonene present but the exact quantity is unknown. bThe menthone found is regarded to be predominantly L-menthone based on the physical testing. It is believed that there might be a smal
26、l amount of D-menthone present but the exact quantity is unknown. cThe carvone foundis regarded to be predominantly L-carvone based on the physical testing. It is believed that there might be a small amount of D-carvone present but the exact quantity is unknown. NOTE The chromatographic profile is n
27、ormative, contrary to typical chromatograms given for information in Annex A. 4.10 Flashpoint Information on the flashpoint is given in Annex B. 5 Sampling See ISO 212. Minimum volume of test sample: 50 ml. NOTE This volume allows each of the tests specified in this part of ISO 3033 to be carried ou
28、t at least once. 6 Test methods 6.1 Relative density at 20 C, 20 20 d See ISO 279. 6.2 Refractive index at 20 C See ISO 280. 6.3 Optical rotation at 20 C See ISO 592. 6.4 Miscibility in ethanol, 70 % (volume fraction), at 20 C See ISO 875. 6.5 Carbonyl value See ISO 1271. Test portion: 1 g. Reflux t
29、ime: 3 h. Relative molar mass of carvone: 150,21. 6.6 Chromatographic profile See ISO 11024-1 and ISO 11024-2. 7 Packaging, labelling, marking and storage See ISO/TR 210 and ISO/TR 211. BS ISO 3033-3:2005 3 Annex A (informative) Typical chromatograms of the analysis by gas chromatography of the esse
30、ntial oil of spearmint, Indian type (Mentha spicata L.), redistilled oil 4 Peak identification Operating conditions 1 Myrcene + 3-octanol 2 Limonene 3 trans-Sabinene hydrate 4 Menthone 5 cis-Dihydrocarvone 6 Carvone 7 trans-Dihydrocarvyl acetate 8 cis-Carvyl acetate 9 cis-Jasmone 10 -Bourbonene 11 V
31、iridiflorol Column: fused silica capillary; length 30 m; internal diameter 0,25 mm Stationary phase: poly(dimethyl siloxane) (DB-1 ) Film thickness: 0,25 m Oven temperature: isothermal at 75 C for 5 min, then temperature programming from 75 C to 100 C at a rate of 5 C/min, then from 100 C to 220 C a
32、t a rate of 6 C/min and isothermal at 220 C for 8,34 min Injector temperature: 230 C Detector temperature: 260 C Detector: flame ionization type Carrier gas: helium Volume injected: 0,1 l Carrier gas flow rate: 1 ml/min Split ratio: 1/100 Figure A.1 Typical chromatogram taken on an apolar column BS
33、ISO 3033-3:20055Peak identification Operating conditions 1 Myrcene 2 Limonene 3 3-Octanol 4 Menthone 5 trans-Sabinene hydrate 6 -Bourbonene 7 cis-Dihydrocarvone 8 trans-Dihydrocarvyl acetate 9 Carvone 10 cis-Carvyl acetate 11 cis-Jasmone 12 Viridiflorol Column: fused silica capillary; length 30 m; i
34、nternal diameter 0,25 mm Stationary phase: poly(ethylene glycol) (DB-WAX ) Film thickness: 0,25 m Oven temperature: isothermal at 75 C for 5 min, then programming temperature from 75 C to 100 C at a rate of 5 C/min, then from 100 C to 220 C at a rate of 6 C/min and isothermal at 220 C for 8,34 min I
35、njector temperature: 230 C Detector temperature: 260 C Detector: flame ionization type Carrier gas: helium Volume injected: 0,1 l Carrier gas flow rate: 1 ml/min Split ratio: 1/100 Figure A.2 Typical chromatogram taken on a polar column BS ISO 3033-3:20056 Annex B (informative) Flashpoint B.1 Genera
36、l information For safety reasons, transport companies, insurance companies, and people in charge of safety services require information on the flashpoints of essential oils, which in most cases are flammable products. A comparative study on the relevant methods of analysis (see ISO/TR 11018) conclud
37、ed that it was difficult to recommend a single apparatus for standardization purposes, given that: there is a wide variation in the chemical composition of essential oils; the volume of the sample needed in certain requirements would be too costly for high- priced essential oils; as there are severa
38、l different types of equipment which can be used for the determination, users cannot be expected to use one specified type only. Consequently, it was decided to give a mean value for the flashpoint in an informative annex to each International Standard, in order to meet the requirements of the inter
39、ested parties. The equipment with which this value was obtained should be specified. For further information, see ISO/TR 11018. B.2 Flashpoint of the essential oil of spearmint, Indian type, redistilled oil The mean value is +58 C. NOTE Obtained with “Setaflash” equipment. BS ISO 3033-3:20057 Biblio
40、graphy 1 ISO/TR 11018, Essential oils General guidance on the determination of flashpoin 2 ISO/TR 21092, Essential oils Characterization BS ISO 3033-3:2005BS ISO 3033-3:2005 BSI 389 Chiswick High Road London W4 4AL BSI British Standards Institution BSI is the independent national body responsible fo
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