1、BS ISO 19824:2017Traditional Chinese medicine Schisandra chinensis (Turcz.) Baill. seeds and seedlingsBSI Standards PublicationWB11885_BSI_StandardCovs_2013_AW.indd 1 15/05/2013 15:06BS ISO 19824:2017 BRITISH STANDARDNational forewordThis British Standard is the UK implementation of ISO 19824:2017.
2、The UK participation in its preparation was entrusted to Technical Committee CH/100/1, Traditional Chinese Medicine.A list of organizations represented on this committee can be obtained on request to its secretary.This publication does not purport to include all the necessary provisions of a contrac
3、t. Users are responsible for its correct application. The British Standards Institution 2017.Published by BSI Standards Limited 2017ISBN 978 0 580 87488 8 ICS 65.020.20 Compliance with a British Standard cannot confer immunity from legal obligations.This British Standard was published under the auth
4、ority of the Standards Policy and Strategy Committee on 31 March 2017.Amendments/corrigenda issued since publicationDate T e x t a f f e c t e dBS ISO 19824:2017 ISO 2017Traditional Chinese medicine Schisandra chinensis (Turcz.) Baill. seeds and seedlingsMdecine traditionnelle chinoise Graines et pl
5、ants de Schisandra chinensis (Turcz.) baill.INTERNATIONAL STANDARDISO19824First edition2017-03Reference numberISO 19824:2017(E)BS ISO 19824:2017ISO 19824:2017(E)ii ISO 2017 All rights reservedCOPYRIGHT PROTECTED DOCUMENT ISO 2017, Published in SwitzerlandAll rights reserved. Unless otherwise specifi
6、ed, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISOs
7、 member body in the country of the requester.ISO copyright officeCh. de Blandonnet 8 CP 401CH-1214 Vernier, Geneva, SwitzerlandTel. +41 22 749 01 11Fax +41 22 749 09 47copyrightiso.orgwww.iso.orgBS ISO 19824:2017ISO 19824:2017(E)Foreword iv1 Scope . 12 Normative references 13 Terms and definitions .
8、 14 Description . 24.1 Schisandra chinensis seeds . 24.2 Schisandra chinensis seedlings . 35 Requirements 45.1 General characteristics . 45.2 Schisandra chinensis seed 45.3 Schisandra chinensis seedling 56 Sampling 56.1 Seed sampling 56.2 Seedling sampling 67 Test methods . 67.1 1 000-seed weight 67
9、.2 Purity . 67.3 Seed width 67.4 Seed viability. 67.5 Stratification rate 67.6 Moisture content . 77.7 Fungus testing . 77.7.1 Preparation of seed 77.7.2 Preparation of seedling 77.7.3 Test of Alternaria spp. . 77.7.4 Test of Fusarium spp. 77.8 Nematode testing 77.9 Stem diameter 77.10 Height of ste
10、m 87.11 Seedling weight 87.12 Number of lateral roots . 88 Test report . 89 Packaging, storage and transportation . 810 Marking 8Annex A (informative) Identification of the Schisandra chinensis seed 10Bibliography .12 ISO 2017 All rights reserved iiiContents PageBS ISO 19824:2017ISO 19824:2017(E)For
11、ewordISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a techn
12、ical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of
13、electrotechnical standardization.The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the different types of ISO documents should be noted. This document w
14、as drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such
15、patent rights. Details of any patent rights identified during the development of the document will be in the Introduction and/or on the ISO list of patent declarations received (see www .iso .org/ patents).Any trade name used in this document is information given for the convenience of users and doe
16、s not constitute an endorsement.For an explanation on the voluntary nature of standards, the meaning of ISO specific terms and expressions related to conformity assessment, as well as information about ISOs adherence to the World Trade Organization (WTO) principles in the Technical Barriers to Trade
17、 (TBT) see the following URL: www .iso .org/ iso/ foreword .html.This document was prepared by ISO/TC 249, Traditional Chinese medicine.iv ISO 2017 All rights reservedBS ISO 19824:2017INTERNATIONAL STANDARD ISO 19824:2017(E)Traditional Chinese medicine Schisandra chinensis (Turcz.) Baill. seeds and
18、seedlings1 ScopeThis document specifies minimum requirements and test methods for Schisandra chinensis (Turcz.) Baill. seeds and seedlings.2 Normative referencesThe following documents are referred to in the text in such a way that some or all of their content constitutes requirements of this docume
19、nt. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.International Seed Testing Association (ISTA):2016, International Rules for Seed TestingInternational Seed Testing Association (ISTA), Wo
20、rking Sheets on Tetrazolium Testing3 Terms and definitionsFor the purposes of this document, the following terms and definitions apply.ISO and IEC maintain terminological databases for use in standardization at the following addresses: IEC Electropedia: available at http:/ www .electropedia. org/ IS
21、O Online browsing platform: available at http:/ www. iso. org/ obp3.1lotspecified quantity of seeds or seedlings that are physically and uniquely identifiable3.2primary sampleportion taken from the lot (3.1) during one single sampling action3.3composite sampleformed by combining and mixing all the p
22、rimary samples (3.2) taken from the lot (3.1)3.4test sampleportion of the composite sample (3.3) to which one of the test required in this document is appliedNote 1 to entry: The test samples (3.4) may be packed in different materials meeting conditions for specific tests e.g. moisture or purity (3.
23、6).3.5sealedcondition whereby a container which contains seeds is closed in such a way that it cannot be opened to get access to the seed and be closed again without either destroying the seal or leaving evidence of tamperingNote 1 to entry: This definition refers to the sealing of seed lots, as wel
24、l as of seed samples. ISO 2017 All rights reserved 1BS ISO 19824:2017ISO 19824:2017(E)3.6purityweight of pure seed fraction over the total weight of the test sample (3.4) in per centNote 1 to entry: The pure seed refers to the species stated by the applicant, or found to predominate in the test, and
25、 includes all botanical varieties and cultivars of that species.3.71 000-seed weightaverage weight of every 1 000 pure seeds of a test sample (3.4)3.8seed widthlargest distance from the side of raphe to its opposite, in millimetreNote 1 to entry: See Figure 1.3.9viabilityindex to show the potential
26、ability of seed to germinate, or the capability of embryo to liveNote 1 to entry: It is expressed as the percentage of stained seeds in the test sample (3.4).Note 2 to entry: It is estimated by the method of Topographical Tetrazolium Test.3.10stratification ratepercentage of stratified seed, determi
27、ned by number, in the test sample (3.4)Note 1 to entry: Stratified seed is the seed with embryos fully grown, endosperm volume expanded, seed coat longitudinally fissured through the raphe by after-ripening treatment (see Figure 1).3.11stem diameterboundary of aboveground and belowground of the seed
28、lingNote 1 to entry: See Figure 2.3.12height of stemlength of the stem from the root to the top hibernaculumNote 1 to entry: See Figure 2.3.13seedling weightaverage weight of a single seedling4 Description4.1 Schisandra chinensis seedsIn this document, Schisandra chinensis seed is the dehydrated see
29、d of Schisandra chinensis (Turcz.) Baill. consisting of three basic parts: embryo, endosperm and seed coat, as shown in Figure 1.NOTE Annex A provides information on how to identify a Schisandra chinensis seed.2 ISO 2017 All rights reservedBS ISO 19824:2017ISO 19824:2017(E)Key1 seed width2 hilum3 ra
30、phe4 seed coat5 embryo6 cavity of seed7 endosperm8 fissure of a stratified seed9 embryo of a stratified seedA seedB kernel, without seed coatC cross-section, longitudinally cut through the rapheD front view of a stratified seedE dorsal view of a stratified seed, shows the fissureF section of the ker
31、nel of a stratified seedFigure 1 Structure of Schisandra chinensis seed4.2 Schisandra chinensis seedlingsSchisandra chinensis seedlings consist of four parts: hibernaculum, stem, lateral root and fibrous root, as shown in Figure 2. ISO 2017 All rights reserved 3BS ISO 19824:2017ISO 19824:2017(E)Key1
32、 hibernaculum2 stem diameter3 lateral root4 fibrous root5 height of stemFigure 2 Structure of Schisandra chinensis 1-year-old seedling5 Requirements5.1 General characteristicsThe following requirements shall be achieved before separating the composite sample into test samples.a) Schisandra chinensis
33、 seed shall be clean and free from foreign odours, with smooth surface and kidney shaped.b) Schisandra chinensis seedling shall be healthy and intact.c) Quarantine fungus (Fusarium spp. and Alternaria spp.) and nematodes shall not be detected.5.2 Schisandra chinensis seed5.2.1 The moisture content s
34、hall not be greater than 14 %.5.2.2 Seed purity shall not be less than 95 %.4 ISO 2017 All rights reservedBS ISO 19824:2017ISO 19824:2017(E)5.2.3 Viability shall not be less than 80 %.5.2.4 The seed width, 1 000-seed weight and stratification rate shall comply with the requirements in Table 1.Table
35、1 Grading standards of Schisandra chinensis seedGradeSeed widthmm1 000-seed weightgStratification rate%First 4,0 26,0 55,0Second 3,6 to 4,0 20,0 to 26,0 30,0 to 55,0Unqualified 3,6 20,0 30,0For the First grade, the seeds, of which the seed width is not less than 4,0 mm, shall not be less than 95 %.
36、Otherwise, it shall be judged to be the Second grade.For the Second grade, the seeds, of which the seed width is not less than 3,6 mm, shall not be less than 95 %. Otherwise, it shall be judged to be the Unqualified grade.NOTE The establishment of the above requirements is based on the seeds collect
37、ed from different regions.5.3 Schisandra chinensis seedling5.3.1 Lateral root and fibrous root shall be intact.5.3.2 Hibernaculum of Schisandra chinensis seedling shall be intact.5.3.3 Stem diameter, height of stem, seedling weight and number of lateral roots shall comply with the requirements in Ta
38、ble 2.Table 2 Grading standards of 1-year-old Schisandra chinensis seedlingGradeStem diametercmHeight of stemcmSeedling weightgNumber of lateral rootsFirst 0,45 20,0 9,0 5,0Second 0,30 to 0,45 15,0 to 20,0 4,0 to 9,0 3,0 to 5,0Unqualified 0,30 15,0 4,0 3,0For the First grade, the seedlings, of which
39、 the stem diameter is not less than 0,45 cm, shall not be less than 95 %. Otherwise, it shall be judged to be the Second grade.For the Second grade, the seedlings, of which the stem diameter is not less than 0,30 cm, shall not be less than 95 %. Otherwise, it shall be judged to be the Unqualified gr
40、ade.NOTE The establishment of the above requirements is based on the seedlings collected from different regions.6 Sampling6.1 Seed samplingSampling shall be carried out in accordance with ISTA, International Rules for Seed Testing: 2016, Chapter 2. Maximum weight of lot and minimum weight of sample
41、are specified in Table 3. ISO 2017 All rights reserved 5BS ISO 19824:2017ISO 19824:2017(E)Table 3 Maximum weight of seed lot and minimum weight of sampleMaximum weight of seed lotkgMinimum weight of samplegComposite sampleTest sample for purity analysisTest sample for other test2 000 600 100 200NOTE
42、 The establishment of the above requirements is based on the seeds collected from different regions.6.2 Seedling samplingThe maximum seedling lot shall be 300 000 seedlings and the minimum composite samples shall be 100 seedlings.7 Test methods7.1 1 000-seed weightDetermination of the 1 000-seed wei
43、ght shall be in accordance with weight determination method specified in ISTA, International Rules for Seed Testing.7.2 PurityDetermination of the seed purity shall be in accordance with the purity analysis of ISTA, International Rules for Seed Testing.7.3 Seed widthTake 100 to 150 intact seeds in d
44、uplicate from the composite sample by quartering method. The seed width shall be measured with a vernier calliper in millimetres (to two decimal places). If the difference between measured values of the duplicate samples is less than 5 %, the seed width of the seed lot shall be the arithmetic mean o
45、f the two measured values; otherwise, it shall be re-measured.7.4 Seed viabilityDetermination of the seed viability shall be in accordance with ISTA, International Rules for Seed Testing and ISTA, Working Sheets on Tetrazolium Testing.7.5 Stratification rateTake duplicate samples of after-ripening t
46、reated seeds by quartering method, each containing 100 to 150 seeds. Calculate the number of seeds with seed coat longitudinally fissured through the raphe by stratification. Formula (1) shall be used to express the stratification rate of a seed lot.MAA()% =m100 (1)6 ISO 2017 All rights reservedBS I
47、SO 19824:2017ISO 19824:2017(E)whereM is stratification rate (%);Amis the number of stratified seeds;A is the number of seeds in the test sample.If the difference between measured maturities of the duplicate samples is less than 5 %, the maturity of the seed lot shall be the arithmetic mean of two me
48、asured values; otherwise, it shall be re-estimated.7.6 Moisture contentDetermination of the mass fraction of moisture shall be in accordance with the high constant temperature method specified in ISTA, International Rules for Seed Testing.7.7 Fungus testing7.7.1 Preparation of seedTake 100 seeds int
49、o a sterilized flask and add 20 ml sterilized water and shake for 8 min. Centrifuge the suspension at 4 000 r/min for 20 min. Re-suspend the residue with 2 ml of sterilized water. Introduce five spots, each spot of 100 l of the final suspension on the blotter or filter paper in a plate for incubation.7.7.2 Preparation of seedlingTake 500 ml sterilized water into a sterilized flask. Take 10 to 20 seedlings. Immerse the seedlings into the flask and shake for 8 min one by one. Centrifuge the suspension at 4 000 r/min for
