1、BSI Standards PublicationBS ISO 19635:2016Fine ceramics (advancedceramics, advanced technicalceramics) Test methodfor antialgal activity ofsemiconducting photocatalyticmaterialsBS ISO 19635:2016 BRITISH STANDARDNational forewordThis British Standard is the UK implementation of ISO 19635:2016.The UK
2、participation in its preparation was entrusted to TechnicalCommittee RPI/13, Advanced technical ceramics.A list of organizations represented on this committee can beobtained on request to its secretary.This publication does not purport to include all the necessaryprovisions of a contract. Users are
3、responsible for its correctapplication. The British Standards Institution 2016.Published by BSI Standards Limited 2016ISBN 978 0 580 86442 1ICS 81.060.30Compliance with a British Standard cannot confer immunity fromlegal obligations.This British Standard was published under the authority of theStand
4、ards Policy and Strategy Committee on 30 April 2016.Amendments/corrigenda issued since publicationDate T e x t a f f e c t e dBS ISO 19635:2016 ISO 2016Fine ceramics (advanced ceramics, advanced technical ceramics) Test method for antialgal activity of semiconducting photocatalytic materialsCramique
5、s techniques Mthode dessai pour lactivit algicide des matriaux photocatalytiques semi-conducteursINTERNATIONAL STANDARDISO19635First edition2016-03-15Reference numberISO 19635:2016(E)BS ISO 19635:2016ISO 19635:2016(E)ii ISO 2016 All rights reservedCOPYRIGHT PROTECTED DOCUMENT ISO 2016, Published in
6、SwitzerlandAll rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can b
7、e requested from either ISO at the address below or ISOs member body in the country of the requester.ISO copyright officeCh. de Blandonnet 8 CP 401CH-1214 Vernier, Geneva, SwitzerlandTel. +41 22 749 01 11Fax +41 22 749 09 47copyrightiso.orgwww.iso.orgBS ISO 19635:2016ISO 19635:2016(E)Foreword ivIntr
8、oduction v1 Scope . 12 Normative references 13 Terms and definitions . 14 Algae used in the test . 25 Preparation for the test . 25.1 Conditions for handling of algae 25.2 Chemicals, materials, and apparatus . 25.3 Sterilization and filtration methods . 35.3.1 Hot-air sterilization . 35.3.2 Hot-stea
9、m sterilization . 35.3.3 Flaming . 45.3.4 Sterilization with alcohol 45.3.5 Sterilization through filtration . 45.4 Culture media . 45.4.1 General 45.4.2 C culture medium 55.4.3 Slant medium 55.4.4 Washing solution . 56 Cultivation of algae . 56.1 Transplantation and cultivation of algae 56.2 Prepar
10、ation of test solution . 67 Photoirradiation method 67.1 Measurement of UV light intensity and preparation of the test piece installation location 67.2 Photoirradiation conditions 68 Test 78.1 Preparation of test piece . 78.2 Cleaning and installation of test pieces . 78.2.1 Cleaning of test pieces
11、. 78.2.2 Installation of test pieces 78.3 Inoculation of test solution. 88.4 Washing of test solution immediately after inoculation of test solution 98.5 Washing of test solution after test 98.6 Measurement according to the three-point absorbance method 98.7 Storage in the dark of test piece on whic
12、h the test solution has been inoculated .109 Test results .109.1 General 109.2 Calculation of antialgal activity on the basis of absorption spectrum method for photocatalytic antialgal materials . 1110 Recording of test results 11Bibliography .12 ISO 2016 All rights reserved iiiContents PageBS ISO 1
13、9635:2016ISO 19635:2016(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested i
14、n a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commis
15、sion (IEC) on all matters of electrotechnical standardization.The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the different types of ISO documents sho
16、uld be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for id
17、entifying any or all such patent rights. Details of any patent rights identified during the development of the document will be in the Introduction and/or on the ISO list of patent declarations received (see www.iso.org/patents).Any trade name used in this document is information given for the conve
18、nience of users and does not constitute an endorsement.For an explanation on the meaning of ISO specific terms and expressions related to conformity assessment, as well as information about ISOs adherence to the WTO principles in the Technical Barriers to Trade (TBT) see the following URL: Foreword
19、- Supplementary information.The committee responsible for this document is ISO/TC 206, Fine ceramics.iv ISO 2016 All rights reservedBS ISO 19635:2016ISO 19635:2016(E)IntroductionUnder ultraviolet (UV) light illumination, photocatalysts have diverse functions, such as prevention of fouling, antifoggi
20、ng effects, antibacterial effects, deodorization, and decomposition of air and water contaminants, and their applications have grown in recent years. Since products utilizing these photocatalytic functions are commercialized in large quantities, a method to evaluate and determine photocatalytic effe
21、cts is required. This International Standard is intended to provide a method for objective evaluation of activity in controlling algae, which are a primary producer of microbial contamination (in the form of environmental biofilms) of outdoor structures, under illumination with ultraviolet light to
22、simulate the outdoor environment and to contribute, via control of algae, to conservation of urban landscapes, prevention of member corrosion, and prevention of fouling of water tank window materials. ISO 2016 All rights reserved vBS ISO 19635:2016BS ISO 19635:2016Fine ceramics (advanced ceramics, a
23、dvanced technical ceramics) Test method for antialgal activity of semiconducting photocatalytic materials1 ScopeThis International Standard specifies a test method for evaluating anti-algal activities in outdoor structures, specifically flat photocatalytic materials (for example, window panes and wa
24、ter tank glasses, films, guardrails, etc.) under irradiation of ultraviolet light. It does not include powder, granular or porous photocatalytic materials.2 Normative referencesThe following documents, in whole or in part, are normatively referenced in this document and are indispensable for its app
25、lication. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.ISO 27447, Fine ceramics (advanced ceramics, advanced technical ceramics) Test method for antibacterial activity of semiconducting
26、photocatalytic materials3 Terms and definitionsFor the purposes of this document, the following terms and definitions apply.3.1photocatalystsubstance with many functions based on oxidization and reduction reactions under photoirradiation (3.3), including decomposition and removal of contaminants, de
27、odorization, antibacterial actions, and prevention of fouling3.2photocatalytic materialsmaterials in which or on which the photocatalyst (3.1) is added by coating, impregnation, mixing, etc.Note 1 to entry: Photocatalytic materials are to be used for building and road construction materials to obtai
28、n the above-mentioned functions.3.3photoirradiationirradiation of UV light at wavelength 300 nm to 380 nm3.4antialgal activityactivity in suppressing growth of algae over a material surface3.5photocatalytic antialgal treatmentsupport of a photocatalyst (3.1) by means of various methods, including co
29、ating, impregnation, blending, and others, for utilization of the antialgal activity of a photocatalystINTERNATIONAL STANDARD ISO 19635:2016(E) ISO 2016 All rights reserved 1BS ISO 19635:2016ISO 19635:2016(E)3.6photocatalytic antialgal activity based on three-point absorbance spectrum methodphotocat
30、alytic antialgal activity (3.4) derived from the ratio of the absorbance of material treated with photocatalyst (3.1) after photoirradiation (3.3) of UV light to that of non-treated material after photoirradiation4 Algae used in the testThe type of algae to be used in the test with photocatalytic-tr
31、eated antialgal materials shall be Chlorella vulgaris (NIES-227). The strain of algae to be used in the test shall be the same strain stored in agencies affiliated with the World Federation for Culture Collections or the Japan Society for Culture Collection.5 Preparation for the test5.1 Conditions f
32、or handling of algaeThe test shall be performed in a laboratory with equipment needed to prevent mixing with other microorganisms.5.2 Chemicals, materials, and apparatusUnless otherwise specified, the chemicals, materials, and apparatus to be used in the test shall be as follows. Test tubes, flasks,
33、 pipettes, tweezers, etc. shall be carefully cleaned with alkaline or neutral detergent, rinsed thoroughly with water, dried, and sterilized by hot air or high-pressure steam before use.5.2.1 Purified water, the water used for the preparation of all solutions and culture media and for all determinat
34、ions shall be distilled or deionized and shall have a conductivity of 1 S/cm.5.2.2 Ethanol for disinfection, solution whose ethanol concentration has been adjusted to 77 % to 82 % in volume fraction by means of purified water.5.2.3 Autoclave, capable of maintaining the temperature at 121 C 1 C (equi
35、valent to a pressure of 103 kPa).5.2.4 Dry sterilizer, capable of maintaining the temperature at 160 C to 180 C.5.2.5 Spectrophotometer, capable of measurement within the wavelength range of 400 nm to 800 nm.5.2.6 Refrigerator, capable of maintaining the temperature at 4 C 1 C.5.2.7 Platinum loop, w
36、ith an end loop of approximately 4 mm.5.2.8 Glass plate, made from material not affecting the growth of microorganisms.5.2.9 Cotton plug, plug of non-degreased cotton, or silicon plug, metal plug, or molten plug.5.2.10 Test tube mixer, for microorganism test.5.2.11 Contact film, film of material not
37、 affecting the growth of microorganisms, which is free of water-absorbing properties and features satisfactory adherence, and in which transmittance in the range of 340 nm to 380 nm, as measured according to the method specified in ISO 27447, accounts for 85 % or more.2 ISO 2016 All rights reservedB
38、S ISO 19635:2016ISO 19635:2016(E)5.2.12 Moisture retention glass, glass with thickness of 1,1 mm or less, in which transmittance of 340 nm to 380 nm, as measured according to the method specified in ISO 27447, accounts for 85 % or more and which has been cut to a size capable of covering the entire
39、surface of a Petri dish.5.2.13 Storage Petri dish, dish with the inside diameter of about 90 mm.5.2.14 Moisture-conditioning filter paper, filter paper not affecting the growth of microorganisms, which has been cut to a size appropriate for placement in the container in which the test piece is to be
40、 set.5.2.15 Disposable cuvette, plastic cuvettes (about 12 mm 12 mm 45 mm) appropriate for placement in the storage container.5.2.16 Tooth brush, soft type of brush made of polyamide.5.2.17 UV fluorescent lamp, 20-W black-light blue type UV fluorescent lamp (BLB lamp) that provides UV-A ranging from
41、 300 nm to 400 nm with a peak emission at 351 nm.5.2.18 Fluorescent lamp, 20-W starter and straight-type white (symbol: W) lamp.5.2.19 UV irradiator, system comprised of one UV fluorescent lamp, which can shield light from the surrounding region.5.2.20 UV light radiometer, radiometer with a detector
42、 whose sensitivity peak is at 351 nm and provide limit, e.g. 10 nm, shall be used. The radiometer shall be calibrated for the light source to be used or corrected to ascertain sensitivity within the wavelength range to be absorbed by the photocatalytic test piece with suitable approach.5.2.21 Illumi
43、nometer, general type AA-grade illuminometer.5.2.22 Filter unit, in which the membrane filter is made from a less-absorbent material, such as hydrophilic polyvinylidene fluoride, hydrophilic polytetrafluoroethylene, etc., and the filter pore size is 0,22 m or 0,45 m.5.2.23 Centrifuge, capable of the
44、 centrifugal force to 1 500 g for preparation of test solutions.5.3 Sterilization and filtration methods5.3.1 Hot-air sterilizationThe apparatus to be sterilized shall be placed in a hot-air sterilizer at 160 C to 180 C and held at this temperature for 30 min to 60 min. If the sterilized sample exhi
45、bits any wet cotton plug or package water after completion of hot-air sterilization, the apparatus shall not be used.5.3.2 Hot-steam sterilizationAn autoclave shall be filled with water and the apparatus to be sterilized shall be placed in a wire cage and placed on the autoclave shelf. The autoclave
46、 shall be closed with the lid and heated and held at a temperature of 121 C (equivalent to a pressure of 103 kPa) for 15 min to 20 min. After discontinuing heating and allowing the temperature to cool to 100 C or less, the exhaust valve shall be opened to bleed steam and the lid shall be opened. The
47、 apparatus sterilized in this fashion shall be taken out and, if necessary, allowed to cool in the safety cabinet.In order to maintain cleanness to prevent contamination by culture media and processing chemicals, the autoclave shall be cleaned with neutral detergent as required and rinsed with water
48、 thoroughly. ISO 2016 All rights reserved 3BS ISO 19635:2016ISO 19635:2016(E)5.3.3 FlamingThe apparatus to be sterilized shall be subjected to gas or alcohol flame treatment. Platinum loops shall be subjected to flame treatment until they become red hot, while test tubes shall be subjected to flame
49、treatment for two to three seconds.5.3.4 Sterilization with alcoholAbsorbent cotton or gauze shall be soaked with ethanol for disinfection and squeezed lightly, for use in wiping both hands.5.3.5 Sterilization through filtrationLiquid to be sterilized shall be filtered with the filter unit.5.4 Culture media5.4.1 GeneralCulture media to be used shall be of the composition shown in Table 1 to Table 3.Table 1 C culture mediumTris (hydroxymethyl) aminomethane 500 mgCa(NO3)24H2O 150 mgKNO3100 mgGlyceric acid disodium 5.5 hyd
