1、Microbiology of the food chain Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp.Part 2: Enumeration method (ISO 11290-2:2017) BS EN ISO 11290-2:2017BSI Standards PublicationWB11885_BSI_StandardCovs_2013_AW.indd 1 15/05/2013 15:06EUROPEAN STANDARD NORM
2、E EUROPENNE EUROPISCHE NORM EN ISO 11290-2 June 2017 ICS 07.100.30 Supersedes EN ISO 11290-2:1998English Version Microbiology of the food chain - Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. - Part 2: Enumeration method (ISO 11290-2:2017) Microbi
3、ologie de la chane alimentaire - Mthode horizontale pour la recherche et le dnombrement de Listeria monocytogenes et de Listeria spp. - Partie 2: Mthode de dnombrement (ISO 11290-2:2017) Mikrobiologie der Lebensmittelkette - Horizontales Verfahren fr den Nachweis und die Zhlung von Listeria monocyto
4、genes und von Listeria spp. - Teil 2: Zhlverfahren (ISO 11290-2:2017) This European Standard was approved by CEN on 6 April 2017. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standa
5、rd without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any
6、 other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Repub
7、lic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. E
8、UROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels 2017 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 11290-2:2
9、017 ENational forewordThis British Standard is the UK implementation of EN ISO 11290-2:2017. It is identical to ISO 11290-2:2017. It supersedes BS EN ISO 11290-2:1998+A1:2004, which is withdrawn.The UK participation in its preparation was entrusted to Technical Committee AW/9, Microbiology.A list of
10、 organizations represented on this committee can be obtained on request to its secretary.This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. The British Standards Institution 2017 Published by BSI Standards Limit
11、ed 2017ISBN 978 0 580 77716 5ICS 07.100.30Compliance with a British Standard cannot confer immunity from legal obligations. This British Standard was published under the authority of the Standards Policy and Strategy Committee on 31 August 2017.Amendments/corrigenda issued since publicationDate Text
12、 affected BRITISH STANDARDBS EN ISO 11290-2:2017EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 11290-2 June 2017 ICS 07.100.30 Supersedes EN ISO 11290-2:1998English Version Microbiology of the food chain - Horizontal method for the detection and enumeration of Listeria monocytogenes and of
13、 Listeria spp. - Part 2: Enumeration method (ISO 11290-2:2017) Microbiologie de la chane alimentaire - Mthode horizontale pour la recherche et le dnombrement de Listeria monocytogenes et de Listeria spp. - Partie 2: Mthode de dnombrement (ISO 11290-2:2017) Mikrobiologie der Lebensmittelkette - Horiz
14、ontales Verfahren fr den Nachweis und die Zhlung von Listeria monocytogenes und von Listeria spp. - Teil 2: Zhlverfahren (ISO 11290-2:2017) This European Standard was approved by CEN on 6 April 2017. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the condit
15、ions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists
16、in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standa
17、rds bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovak
18、ia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels 2017 CEN All rights of exploitation in any form and by any means
19、reserved worldwide for CEN national Members. Ref. No. EN ISO 11290-2:2017 EBS EN ISO 11290-2:2017EN ISO 11290-2:2017 (E) 3 European foreword This document (EN ISO 11290-2:2017) has been prepared by Technical Committee ISO/TC 34 ” Food products” in collaboration with Technical Committee CEN/TC 275 “F
20、ood analysis - Horizontal methods” the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by December 2017, and conflicting national standards shall be withdrawn at
21、the latest by December 2017. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN shall not be held responsible for identifying any or all such patent rights. This document supersedes EN ISO 11290-2:1998. This document has been pre
22、pared under a mandate given to CEN by the European Commission and the European Free Trade Association. According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia
23、, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey
24、and the United Kingdom. Endorsement notice The text of ISO 11290-2:2017 has been approved by CEN as EN ISO 11290-2:2017 without any modification. ISO 11290-2:2017(E)Foreword ivIntroduction v1 Scope . 12 Normative references 13 Terms and definitions . 24 Principle 24.1 General . 24.2 Initial suspensi
25、on 24.3 Surface plating . 24.4 Incubation . 24.5 Confirmation . 34.6 Enumeration . 35 Culture media and reagents 36 Equipment and consumables 37 Sampling 38 Preparation of test sample . 49 Procedure. 49.1 Test portion, initial suspension and dilutions 49.2 Inoculation and incubation 49.3 Enumeration
26、 of characteristic colonies . 49.4 Confirmation of L. monocytogenes or Listeria spp. 59.4.1 Selection of colonies for confirmation 59.4.2 Confirmation of L. monocytogenes . 69.4.3 Confirmation of Listeria spp. 99.5 Interpretation of morphological and physiological properties and of the biochemical r
27、eactions 109.6 Additional characterization of isolated strains (optional) .1010 Expression of results .1011 Performance characteristics of the method 1011.1 Method validation study 1011.2 Repeatability limit . 1111.3 Reproducibility limit . 1112 Test report 1113 Quality assurance .12Annex A (normati
28、ve) Diagram of procedure .13Annex B (normative) Composition and preparation of media and reagents 14Annex C (informative) Distinction of Listeria spp. from other genera .22Annex D (informative) Reactions for the identification of Listeria species 23Annex E (informative) Results of interlaboratory st
29、udies for enumeration of Listeria monocytogenes .25Bibliography .28 ISO 2017 All rights reserved iiiContents PageBS EN ISO 11290-2:2017ISO 11290-2:2017(E)Foreword ivIntroduction v1 Scope . 12 Normative references 13 Terms and definitions . 24 Principle 24.1 General . 24.2 Initial suspension 24.3 Sur
30、face plating . 24.4 Incubation . 24.5 Confirmation . 34.6 Enumeration . 35 Culture media and reagents 36 Equipment and consumables 37 Sampling 38 Preparation of test sample . 49 Procedure. 49.1 Test portion, initial suspension and dilutions 49.2 Inoculation and incubation 49.3 Enumeration of charact
31、eristic colonies . 49.4 Confirmation of L. monocytogenes or Listeria spp. 59.4.1 Selection of colonies for confirmation 59.4.2 Confirmation of L. monocytogenes . 69.4.3 Confirmation of Listeria spp. 99.5 Interpretation of morphological and physiological properties and of the biochemical reactions 10
32、9.6 Additional characterization of isolated strains (optional) .1010 Expression of results .1011 Performance characteristics of the method 1011.1 Method validation study 1011.2 Repeatability limit . 1111.3 Reproducibility limit . 1112 Test report 1113 Quality assurance .12Annex A (normative) Diagram
33、 of procedure .13Annex B (normative) Composition and preparation of media and reagents 14Annex C (informative) Distinction of Listeria spp. from other genera .22Annex D (informative) Reactions for the identification of Listeria species 23Annex E (informative) Results of interlaboratory studies for e
34、numeration of Listeria monocytogenes .25Bibliography .28 ISO 2017 All rights reserved iiiContents PageBS EN ISO 11290-2:2017ISO 11290-2:2017(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of prep
35、aring International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in
36、liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC
37、Directives, Part 1. In particular the different approval criteria needed for the different types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).Attention is drawn to the possibility
38、that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the document will be in the Introduction and/or on the ISO list of patent
39、 declarations received (see www .iso .org/ patents).Any trade name used in this document is information given for the convenience of users and does not constitute an endorsement.For an explanation on the meaning of ISO specific terms and expressions related to conformity assessment, as well as infor
40、mation about ISOs adherence to the World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see the following URL: www .iso .org/ iso/ foreword .html.This document was prepared by the European Committee for Standardization (CEN) Technical Committee CEN/TC 275, Food analysis
41、 Horizontal methods, in collaboration with ISO Technical Committee ISO/TC 34, Food products, Subcommittee SC 9, Microbiology, in accordance with the agreement on technical cooperation between ISO and CEN (Vienna Agreement).This second edition cancels and replaces the first edition (ISO 11290-2:1998)
42、, which has been technically revised. It also incorporates the amendment ISO 11290-2:1998/Amd.1:2004. The main changes, compared to ISO 11290-2:1998, are the following. The enumeration of Listeria monocytogenes has been modified as listed below. Primary suspension with buffered peptone water, half-F
43、raser broth with or without supplements, and all appropriate diluents referred to in ISO 6887 (all parts). Resuscitation step deleted. Microscopic aspect, catalase and CAMP test for confirmation are optional. Inclusion of new performance characteristics. Moreover, enumeration of Listeria spp. has be
44、en included in the scope and the title changed accordingly.A list of parts in the ISO 11290 series can be found on the ISO website.iv ISO 2017 All rights reservedBS EN ISO 11290-2:2017ISO 11290-2:2017(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of nat
45、ional standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. Interna
46、tional organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.The procedures used to develop this document and those intend
47、ed for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the different types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www .
48、iso .org/ directives).Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the document
49、will be in the Introduction and/or on the ISO list of patent declarations received (see www .iso .org/ patents).Any trade name used in this document is information given for the convenience of users and does not constitute an endorsement.For an explanation on the meaning of ISO specific terms and expressions related to conformity assessment, as well as information about ISOs adherence to the World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see the following URL: www .iso .org/ iso/ foreword .html.This do
