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    ASTM E969-2002(2007) 923 Standard Specification for Glass Volumetric (Transfer) Pipets《玻璃体积(移液)管标准规范》.pdf

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    ASTM E969-2002(2007) 923 Standard Specification for Glass Volumetric (Transfer) Pipets《玻璃体积(移液)管标准规范》.pdf

    1、Designation: E 969 02 (Reapproved 2007)Standard Specification forGlass Volumetric (Transfer) Pipets1This standard is issued under the fixed designation E 969; the number immediately following the designation indicates the year oforiginal adoption or, in the case of revision, the year of last revisio

    2、n. A number in parentheses indicates the year of last reapproval. Asuperscript epsilon (e) indicates an editorial change since the last revision or reapproval.This standard has been approved for use by agencies of the Department of Defense.1. Scope1.1 This specification covers volumetric pipets of t

    3、woclasses. ClassA, Precision Pipet and Class B, General Purpose.NOTE 1Specifications for micropipets are given in SpecificationE 193.1.2 Product with a stated capacity not listed in this standardmay be specified class A tolerance when product conforms tothe tolerance range of the next smaller volume

    4、tric standardproduct listed in Table 1.2. Referenced Documents2.1 ASTM Standards:2E 193 Specification for Laboratory Glass MicropipetsE 438 Specification for Glasses in Laboratory ApparatusE 920 Specification for Commercially Packaged LaboratoryApparatusE 921 Specification for Export Packaged Labora

    5、tory Appa-ratusE 1133 Practice for Performance Testing of Packaged Labo-ratory Apparatus for United States Government Procure-mentsE 1157 Specification for Sampling and Testing of ReusableLaboratory Glassware3. General Requirements3.1 Borosilicate GlassBorosilicate glass for pipets shallconform to t

    6、he glass requirements of Type 1, Class A or B ofSpecification E 438.3.2 CalibrationPipets shall be calibrated to deliver (sym-bol TD) the intended capacity at 20C. The pipet shall be filledabout 20 mm above the capacity line. The water is loweredslowly to the capacity line. Delivery of the contents

    7、into areceiving vessel is made with the tip in contact with the wall ofthe vessel and no after-drainage period is allowed. Accuracyshall be within the limits specified in Table 1.4. Design4.1 ShapeThe pipets shall consist in general of a suctiontube and a delivery tube separated by a bulb; all three

    8、 partsshall be permanently attached together. Any cross-section ofthe pipet taken in a plane perpendicular to the longitudinal axisshall be circular. The shape shall permit complete emptyingand thorough cleaning.4.1.1 BulbThe shape shall permit complete emptyingwithout any hold up, and easy cleaning

    9、.4.2 DimensionsThe length of the suction tube shall be150 to 190 mm and the minimum wall thickness of bothsuction and delivery tubes shall be 0.90 mm. Pipets mustcomply with the essential dimensions given in Table 1.4.3 Delivery TipsDelivery tips shall be made with agradual taper of 1.5 to 3 cm. The

    10、 end of the tip shall beperpendicular to the longitudinal axis of the tip. The outsideedge of the tip may be bevelled slightly and the end and thebevel shall be ground or fire-polished. Sudden constriction atthe orifice would impair smooth flow characteristics of thedelivery stream and is not accept

    11、able.4.3.1 Tempered TipsMay be supplied at option of manu-facturer. The tempered tip, when tested in index oil whichmatches the refractive index of the glass being used, shall havea temper between 75 and 220 nm (millimicrons).4.4 MarkingsAll markings shall be permanent and leg-ible.4.4.1 Capacity Li

    12、neThe capacity line shall be a sharplydefined line of uniform width (maximum 0.6 mm) in a planeperpendicular to the vertical axis of the pipet. The line shall beapplied by one of the following methods: etched and filled with1This specification is under the jurisdiction of ASTM Committee E41 onLabora

    13、tory Apparatus and is the direct responsibility of Subcommittee E41.01 onApparatus.Current edition approved Nov. 1, 2007. Published December 2007. Originallyapproved in 1983. Last previous edition approved in 2002 as E 969 02.2For referenced ASTM standards, visit the ASTM website, www.astm.org, orco

    14、ntact ASTM Customer Service at serviceastm.org. For Annual Book of ASTMStandards volume information, refer to the standards Document Summary page onthe ASTM website.1Copyright ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.a permanent pigment;

    15、 etched through a vertical colored stripefused into the glass; by application of a stain fired into the glasswithout etching; or by application of an enamel fired onto theglass without etching. The line shall completely encircle thetube except when the line is a stain fired into the glass withoutetc

    16、hing. If so stained, the line shall extend a minimum of 90 %of the way around the tube.4.4.2 Color CodingEach pipet shall be marked with oneor two color bands as specified in Table 1. The single bandshould be 6 to 10 mm wide, while the double color bandsshould each be 3 to 5 mm wide and separated by

    17、 a clear spaceof 2 to 3 mm. Color bands do not have to be continuous butmay be broken as long as they cover 40 % of the circumferenceof the pipet. Colors must be bright and distinct.4.4.3 Identification MarkingsEach pipet marked by oneof the methods given in 4.4.1 shall show the manufacturersname or

    18、 trademark, the nominal capacity, the word “delivers”or the symbol “TD,” the temperature of calibration, 20C. Theletter “A” symbolizing Class A accuracy to appear on all ClassA pipets. Class A pipets may be optionally serialized.4.5 WorkmanshipPipets shall be free of defects whichmay distort the app

    19、earance of the liquid surface or may impairthe serviceability.5. Sampling and Testing5.1 For sampling refer to Specification E 1157.6. Packaging6.1 For packaging, select from Specifications E 920, E 921,or Practice E 1133.7. Keywords7.1 glass; pipets; volumetricASTM International takes no position r

    20、especting the validity of any patent rights asserted in connection with any item mentionedin this standard. Users of this standard are expressly advised that determination of the validity of any such patent rights, and the riskof infringement of such rights, are entirely their own responsibility.Thi

    21、s standard is subject to revision at any time by the responsible technical committee and must be reviewed every five years andif not revised, either reapproved or withdrawn. Your comments are invited either for revision of this standard or for additional standardsand should be addressed to ASTM Inte

    22、rnational Headquarters. Your comments will receive careful consideration at a meeting of theresponsible technical committee, which you may attend. If you feel that your comments have not received a fair hearing you shouldmake your views known to the ASTM Committee on Standards, at the address shown

    23、below.This standard is copyrighted by ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959,United States. Individual reprints (single or multiple copies) of this standard may be obtained by contacting ASTM at the aboveaddress or at 610-832-9585 (phone), 610-832-95

    24、55 (fax), or serviceastm.org (e-mail); or through the ASTM website(www.astm.org).TABLE 1 Requirements for Volumetric (Transfer) PipetNominalCapacity,mLCapacity Tolerance,mLLength of Delivery Tube,mmInside Diameter atCapacity Mark, mmMaximum DistanceBetween Bulb andGraduation Mark, mmMinimum OutflowT

    25、ime, sA Color-CodingBandClass A Class B min max min max Class A Class B0.5 60.006 60.012 100 140 1.3 4 60 5 3 black (2)1 60.006 60.012 100 140 2 4 60 8 3 blue2 60.006 60.012 120 150 2 4 60 8 3 orange3 60.01 60.02 130 170 2 4 60 8 5 black4 60.01 60.02 130 170 2 4 60 8 5 red (2)5 60.01 60.02 130 170 2

    26、 4 60 8 8 white6 60.01 60.03 150 190 2 4 60 10 8 orange (2)7 60.01 60.03 150 190 2 4 60 10 8 green (2)8 60.02 60.04 150 190 2 4 60 10 8 blue9 60.02 60.04 150 190 2 4 60 10 8 black10 60.02 60.04 150 190 2 4 70 15 8 red15 60.03 60.06 180 220 3.5 5.5 70 25 10 green20 60.03 60.06 230 260 3.5 5.5 70 25 10 yellow25 60.03 60.06 230 260 3.5 5.5 70 25 15 blue30 60.03 . 230 260 3.5 5.5 70 25 . black40 60.05 . 230 260 4 6 70 25 . white50 60.05 60.10 230 260 4 6 70 25 15 red100 60.08 60.16 230 260 5 7 80 30 20 yellowAMaximum outflow time for A or B shall be 60 s.E 969 02 (2007)2


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