1、Designation: C 1338 08Standard Test Method forDetermining Fungi Resistance of Insulation Materials andFacings1This standard is issued under the fixed designation C 1338; the number immediately following the designation indicates the year oforiginal adoption or, in the case of revision, the year of l
2、ast revision. A number in parentheses indicates the year of last reapproval. Asuperscript epsilon () indicates an editorial change since the last revision or reapproval.1. Scope1.1 This test method covers the determination of the abilityof new insulation materials and their facings to support fungal
3、growth.1.2 This standard does not purport to address all of thesafety concerns, if any, associated with its use. It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use.1.3 The
4、values stated in inch-pound units are to be regardedas standard. The values given in parentheses are mathematicalconversions to SI units that are provided for information onlyand are not considered standard.2. Significance and Use2.1 The type of materials used in the manufacture ofinsulation product
5、s and the type of membrane used to facethese products can sometimes affect fungi sustenance in thepresence of high humidity.2.2 This test method is used to determine the relative abilityof an insulation and its facing to support or resist fungal growthunder conditions favorable for their development
6、.2.3 This test method uses a comparative material to deter-mine the relative ability of a material to support fungal growth.In some specialized product areas, it is required that no growthtake place. In such cases, the use of the comparative materialis omitted and the pass/fail criterion is based up
7、on growth.3. Apparatus3.1 GlasswareSterile disposable petri dishes, 4 or 6 in.(100 or 150 mm) by 0.6 or 0.75 in. (15 or 20 mm) in size arepreferred. For larger specimens, trays of borosilicate glass orbaking dishes up to 16 by 20 in. (400 by 600 mm) in size maybe used.3.2 Environmental Chamber or Ca
8、binetEquipment forthis test method shall maintain a temperature of 82.4 to 86F(28 to 30C) and a relative humidity of 95 % (64 %).Provisions shall be made to prevent condensation from drip-ping on the test specimen. There shall be free circulation of airaround the test chamber.3.3 AtomizerA chromatog
9、raphy atomizer capable of pro-viding 100 000 6 20 000 spores/in.2(15 000 6 3000 spores/cm2) shall be used for inoculation.3.4 Autoclavable Biohazard Bags, or metal pan able towithstand autoclaving.4. Reagents and Materials4.1 Purity of WaterUnless otherwise specified, referencesto water shall be und
10、erstood to mean sterile distilled water orwater of equal purity.4.2 Inoculum:Fungi ATCC2Aspergillus niger 9642Aspergillus versicolor 11 730Penicillium funiculosum 11 797Chaetomium globosum 6205Aspergillus flavus 96434.3 CulturesMaintain cultures of the Aspergillus fungiseparately on Czapek Dox agar
11、(see Note 1). Culture theChaetomium globosum on strips of cellulose filter paper on thesurface of Czapek Dox agar. Maintain the Penicillium fungi onSabouraud Dextrose agar. The stock cultures may be kept fornot more than 4 months at 43 6 7F (6 6 4C) at which timesubcultures shall be made, and new st
12、ocks selected from thesubcultures. If genetic or physiological changes occur, obtainnew cultures. Incubate subcultures used for preparing newstock cultures or the spore suspension at 86 6 4F (30 6 2C)for 5 days or longer.NOTE 1This media is readily available from any science/microbiological supply h
13、ouse.5. Specimens5.1 Viability SpecimensDetermine the viability of thespore suspension during incubation with these controls: with1This test method is under the jurisdiction ofASTM Committee C16 on ThermalInsulation and is the direct responsibility of Subcommittee C16.31 on Chemical andPhysical Prop
14、erties.Current edition approved June 1, 2008. Published July 2008. Originally approvedin 1996. Last previous edition approved in 2000 as C 133800.2The sole source of supply of the cultures known to the committee at this timeis American Type Culture Collection (ATCC), 12301 Parklawn Drive, Rockville,
15、MD 20852. If you are aware of alternative suppliers, please provide this informationto ASTM International Headquarters. Your comments will receive careful consid-eration at a meeting of the responsible technical committee,1which you may attend.1Copyright ASTM International, 100 Barr Harbor Drive, PO
16、 Box C700, West Conshohocken, PA 19428-2959, United States.each daily group of tests, place one piece of sterilized What-man 500 filter paper, 1 in.2(6.4 cm2) on each of two preparedhardened Czapek Dox agar specimens in separate petri dishes.Prepare a third petri dish with Sabouraud Dextrose agar.5.
17、2 Comparative MaterialA white birch tongue depres-sor, 0.75 by 6 in. (20 by 150 mm) or southern yellow pine is thecomparative item to determine the relative growth on speci-mens being tested. The choice of comparative item should benoted. Refer to the appropriate materials standard for relevantcompa
18、rative materials.NOTE 2The comparative item is chosen to reflect the buildingconstruction material. In some cases, other material may be more relevantas a comparative item.5.3 Test Specimens:5.3.1 Prepare triplicate specimens from each test sample.5.3.1.1 If the test sample is of different construct
19、ion on eachface, prepare triplicate specimens of each face in the upposition.5.3.2 The preferred specimen thickness is 0.75 in. (20 mm).If altering the specimen thickness is required, ensure that thesurface to be tested has not been altered and is in the upposition. If the normal specimen thickness
20、is less than 0.75 in.(20 mm), then test at its normal thickness. If the specimencontainer will be covered, the specimen shall not make contactwith it.5.3.2.1 When testing loose materials such as blowing orpouring insulations, refer to the appropriate materials standardfor sample preparation recommen
21、dations.5.3.3 It is sometimes desirable to test the adhesive used tobond a facing to the substrate. In such case only, peel back thefacing approximately half way across the face of the specimenbefore testing. Note this change clearly in the report.6. Procedure6.1 Spore SuspensionPrepare a spore susp
22、ension of eachof the five fungi by pouring into one subculture of each fungusa 10 mL portion of a sterile solution containing a sufficientquantity, not to exceed 0.10 g/L, of a nontoxic wetting agentsuch as sorbitan monoleate (Tween-80), sodium dioctyl sulfo-succinate, or sodium lauryl sulfate to pr
23、event clumping of thespores. Gently scrape the surface growth from the culture ofthe test organism using a sterile platinum or nichrome inocu-lating wire. Pour the spore charge into a sterile 125-mLglass-stoppered Erlenmeyer flask containing 45 6 1mLofsterile water, and 50 to 75 solid glass beads, a
24、pproximately0.20 in. (5 mm) in diameter. Vigorously shake the flask toliberate the spores from the fruiting bodies and to break thespore clumps. Filter the dispersed fungal spore suspensionthrough at least a 0.24-in. (6-mm) layer of glass wool containedin a glass funnel, into a sterile flask. This p
25、rocess is intended toremove large mycelial fragments and clumps of agar that couldinterfere with the spraying process. Centrifuge the filteredspore suspension. Remove all the supernatant down to thesurface of the spore pellet, taking care not to remove or disturbthe spore pellet. Resuspend the resid
26、ue in 50 mL of sterilewater and centrifuge. (It may be necessary to add a smallquantity of nontoxic wetting agent, not to exceed 0.10 g/L, toprevent clumping of the spores.) Wash the spores obtainedfrom each of the fungi in this manner three times. Dilute thefinal washed residue with distilled water
27、 in such a manner thatthe resultant spore suspension shall contain 1 000 0006200 000 spores per mL as determined with a counting chamber.Repeat the operation for each organism used in the test andblend equal volumes of the resultant spore suspensions toobtain the final mixed spore suspension. The sp
28、ore suspensionmay be prepared fresh each day or may be held at 43 6 7F (66 4C) for not more than 28 days, or until the viability testindicates poor growth, or until growth appears in the sealedstorage bottle.6.2 Inoculation of Test Specimens, Comparative Material,and Control SpecimensPrecondition th
29、e chamber and itscontents at 866 4F (30 6 2C) and 956 4 % relativehumidity for at least 4 h. Place each test, comparative material,and viability control specimen in separate sterile petri dishes.Inoculate each specimen with approximately 0.50 mL of sporesuspension by spraying exposed surfaces in the
30、 form of a finemist from a previously sterilized atomizer or nebulizer. If aspecimen container is covered, it shall be loose fitting glass toallow air circulation. Place specimens in the chamber andimmediately begin incubation.6.3 IncubationMaintain the test chamber at 86 6 4F (306 2C) and a relativ
31、e humidity of 95 6 4 % throughout thetest. Keep the test chamber closed during incubation exceptduring inspection. After 3 to 7 days, inspect the controlspecimens. If control specimens do not show an abundance ofgrowth at this time, repeat the entire test. If growth is presenton the control specimen
32、s, continue the test for a minimumperiod of 28 days 6 8 h from the time of incubation. Somematerials may require longer periods of incubation, therefore,the test period may be extended. Note all incubation times.Refer to the materials specifications for incubation periods.7. Interpretation of Result
33、s7.1 InspectionAt the end of the incubation period, removethe test specimens and comparative item from the test chamberand examine at 403 magnification.7.2 Interpretation of ResultsTest specimens that havegrowth greater than that on the comparative item shall beconsidered to have failed. Test specim
34、ens on which the growthis not greater than that on the comparative item shall beconsidered to have passed.7.2.1 If no growth is the criterion, the observation of anygrowth on the test specimen shall be considered a failure.7.3 After completion of inspection, all test specimens andtest equipment shal
35、l be autoclaved under the manufacturersautoclave instructions to ensure destruction of vegetative cellsand spores to prevent accidental contamination to the labora-tory and environment.8. Report8.1 Report the following information:8.1.1 Complete identification of the material tested,8.1.2 Identifica
36、tion of variable test conditions (comparativeitem, sample moistening, test duration) criterion used todetermine Pass/Fail (comparative item or no growth), and8.1.3 Results of the test.C13380829. Precision and Bias9.1 No statement is made about either the precision or thebias of this fungi resistance
37、 test method since the test is asubjective, visual determination of whether the test materialdiffers from a comparative material.10. Keywords10.1 batts; blanket; boards; cellulose; facings; foam; fungiresistance; insulation; loose-fill; mineral fiberASTM International takes no position respecting th
38、e validity of any patent rights asserted in connection with any item mentionedin this standard. Users of this standard are expressly advised that determination of the validity of any such patent rights, and the riskof infringement of such rights, are entirely their own responsibility.This standard i
39、s subject to revision at any time by the responsible technical committee and must be reviewed every five years andif not revised, either reapproved or withdrawn. Your comments are invited either for revision of this standard or for additional standardsand should be addressed to ASTM International He
40、adquarters. Your comments will receive careful consideration at a meeting of theresponsible technical committee, which you may attend. If you feel that your comments have not received a fair hearing you shouldmake your views known to the ASTM Committee on Standards, at the address shown below.This s
41、tandard is copyrighted by ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959,United States. Individual reprints (single or multiple copies) of this standard may be obtained by contacting ASTM at the aboveaddress or at 610-832-9585 (phone), 610-832-9555 (fax), or serviceastm.org (e-mail); or through the ASTM website(www.astm.org).C1338083
