1、BS ISO 8897:2010 ICS 71.100.60 NO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAW BRITISH STANDARD Oil of juniper berry (Juniperus communis L.)This British Standard was published under the authority of the Standards Policy and Strategy Committee on 31 July 2010 BSI 2010 ISBN 978
2、0 580 62696 8 Amendments/corrigenda issued since publication Date Comments BS ISO 8897:2010 National foreword This British Standard is the UK implementation of ISO 8897:2010. The UK participation in its preparation was entrusted to Technical Committee AW/54, Essential oils. A list of organizations r
3、epresented on this committee can be obtained on request to its secretary. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. Compliance with a British Standard cannot confer immunity from legal obligations.BS IS
4、O 8897:2010Reference number ISO 8897:2010(E) ISO 2010INTERNATIONAL STANDARD ISO 8897 Second edition 2010-06-01 Oil of juniper berry (Juniperus communis L.) Huile essentielle de baies de genvrier (Juniperus communis L.) BS ISO 8897:2010 ISO 8897:2010(E) PDF disclaimer This PDF file may contain embedd
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9、ter. ISO copyright office Case postale 56 CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail copyrightiso.org Web www.iso.org Published in Switzerland ii ISO 2010 All rights reservedBS ISO 8897:2010 ISO 8897:2010(E) ISO 2010 All rights reserved iiiForeword ISO (the International O
10、rganization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been estab
11、lished has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardizat
12、ion. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting
13、. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such p
14、atent rights. ISO 8897 was prepared by Technical Committee ISO/TC 54, Essential oils. This second edition cancels and replaces the first edition (ISO 8897:1991), which has been technically revised. BS ISO 8897:2010BS ISO 8897:2010 INTERNATIONAL STANDARD ISO 8897:2010(E) ISO 2010 All rights reserved
15、1Oil of juniper berry (Juniperus communis L.) 1 Scope This International Standard specifies certain characteristics of oil of juniper berry (Juniperus communis L.), with a view to facilitating the assessment of its quality. 2 Normative references The following referenced documents are indispensable
16、for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO/TR 210, Essential oils General rules for packaging, conditioning and storage ISO/TR 211, Essential
17、 oils General rules for labelling and marking of containers ISO 212, Essential oils Sampling ISO 279, Essential oils Determination of relative density at 20 C Reference method ISO 280, Essential oils Determination of refractive index ISO 592, Essential oils Determination of optical rotation ISO 875,
18、 Essential oils Evaluation of miscibility in ethanol ISO 1242, Essential oils Determination of acid value ISO 11024-1, Essential oils General guidance on chromatographic profiles Part 1: Preparation of chromatographic profiles for presentation in standards ISO 11024-2, Essential oils General guidanc
19、e on chromatographic profiles Part 2: Utilization of chromatographic profiles of samples of essential oils 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 essential oil of juniper berry essential oil obtained by steam distillation of the fres
20、h, dried or fermented berries of Juniperus communis L., of the Cupressaceae family NOTE For information on CAS number, see ISO/TR 21092 2 . BS ISO 8897:2010 ISO 8897:2010(E) 2 ISO 2010 All rights reserved4 Requirements 4.1 Appearance Liquid. 4.2 Colour Colourless, pale green, pale yellow. 4.3 Odour
21、Fresh, warm, balsamic, and sweet-woody. 4.4 Relative density at 20 C, 20 20 d Minimum: 0,850. Maximum: 0,880. 4.5 Refractive index at 20 C Minimum: 1,470. Maximum: 1,483. 4.6 Optical rotation at 20 C Between 0 and 16. 4.7 Miscibility in 95 % (volume fraction) ethanol at 20 C It shall not be necessar
22、y to use more than 10 volumes of 95 % (volume fraction) ethanol to obtain a clear solution with 1 volume of essential oil. 4.8 Acid value Maximum: 2. 4.9 Chromatographic profile Carry out the analysis of the essential oil by gas chromatography. Identify in the chromatogram obtained, the representati
23、ve and characteristics components listed in Table 1. The proportions of each of these components, indicated by the integrator, shall be as shown in Table 1. This constitutes the chromatographic profile of the essential oil. 4.10 Flashpoint Information on the flashpoint is given in Annex B. BS ISO 88
24、97:2010 ISO 8897:2010(E) ISO 2010 All rights reserved 3Table 1 Chromatographic profile Component Minimum % Maximum % -Pinene 25,0 45,0 Sabinene 4,0 20 -Pinene 1,0 12 Myrcene 3,0 22,0 Limonene 2,0 8,0 1-Terpinen-4-ol 1,0 6,0 n-Bornyl acetate n.d. a0,6 -Caryophyllene 1,5 5,0 -Humulene 1,0 4,0 Germacre
25、ne D 1,0 5,0 -Cadinene 1,0 3,5 NOTE The chromatographic profile is normative, contrary to typical chromatograms given for information in Annex A. aNot detectable. 5 Sampling See ISO 212. Minimum volume of the final sample: 25 ml. NOTE This volume allows each of the tests specified in this Internatio
26、nal Standard to be carried out at least once. 6 Test methods 6.1 Relative density at 20 C, 20 20 d See ISO 279. 6.2 Refractive index at 20 C See ISO 280. 6.3 Optical rotation at 20 C See ISO 592. 6.4 Miscibility with 95 % (volume fraction) ethanol at 20 C See ISO 875. BS ISO 8897:2010 ISO 8897:2010(
27、E) 4 ISO 2010 All rights reserved6.5 Acid value See ISO 1242. 6.6 Chromatographic profile See ISO 11024-1 and ISO 11024-2. 7 Packaging, labelling and marking See ISO/TR 210 and ISO/TR 211. BS ISO 8897:2010 ISO 8897:2010(E) ISO 2010 All rights reserved 5Annex A (informative) Typical chromatograms of
28、the analysis by gas chromatography of the essential oil of juniper berry (Juniperus communis L.) Peak identification Operating conditions 1 -Pinene 2 -Pinene 3 Sabinene 4 Myrcene 5 Limonene 6 1-Terpinen-4-ol 7 n-Bornyl acetate 8 -Caryophyllene 9 -Humulene 10 Germacrene D 11 -Cadinene Column: silica
29、capillary; length 50 m; internal diameter 0,20 mm Stationary phase: polydimethylsiloxane Film thickness: 0,25 m Oven temperature: 65 C to 230 C, at a rate of 2 C/min Injector temperature: 230 C Detector temperature: 250 C Detector: flame ionization type Carrier gas: hydrogen Volume injected: 0,2 l S
30、plit ratio: 1:100 t time V response Figure A.1 Typical chromatogram taken on an apolar column BS ISO 8897:2010 ISO 8897:2010(E) 6 ISO 2010 All rights reservedPeak identification Operating conditions 1 -Pinene 2 -Pinene 3 Sabinene 4 Myrcene 5 Limonene 6 n-Bornyl acetate 7 1-Terpinen-4-ol 8 -Caryophyl
31、lene 9 Germacrene D 10 -Cadinene Column: silica capillary; length 50 m; internal diameter 0,20 mm Stationary phase: polyethylene glycol Film thickness: 0,25 m Oven temperature: 65 C to 230 C, at a rate of 2 C/min Injector temperature: 230 C Detector temperature: 250 C Detector: flame ionization type
32、 Carrier gas: hydrogen Volume injected: 0,2 l Split ratio: 1:100 Carrier gas flow rate: 1 ml/min t time V response Figure A.2 Typical chromatogram taken on a polar column BS ISO 8897:2010 ISO 8897:2010(E) ISO 2010 All rights reserved 7Annex B (informative) Flashpoint B.1 General information For safe
33、ty reasons, transport companies, insurance companies, and people in charge of safety services require information on the flashpoints of essential oils, which in most cases are flammable products. A comparative study on the relevant methods of analysis (see ISO/TR 11018 1 ) concluded that it was diff
34、icult to recommend a single apparatus for standardization purposes, given that: there is a wide variation in the chemical composition of essential oils; the volume of the sample needed in certain equipment is incompatible with the high price of essential oils; as there are several different types of
35、 equipment which can be used for the determination, users cannot be expected to use one specified type only. Consequently, it was decided to give a mean value for the flashpoint annexed to each International Standard, for information, in order to meet the requirements of the interested parties. The
36、equipment with which this value was obtained has to be specified. For further information see ISO/TR 11018 1 . B.2 Flashpoint of the essential oil of juniper berry (Juniperus communis L.) The mean value is +41 C. NOTE Obtained with “Setaflash” 1)equipment. 1) Product available commercially. This inf
37、ormation is given for the convenience of users of this document and does not constitute an endorsement by ISO of this product. BS ISO 8897:2010 ISO 8897:2010(E) 8 ISO 2010 All rights reservedBibliography 1 ISO/TR 11018, Essential oils General guidance on the determination of flashpoint 2 ISO/TR 2109
38、2, Essential oils Characterization BS ISO 8897:2010 This page has been intentionally left blankBS ISO 8897:2010 BSI Group Headquarters 389 Chiswick High Road, London, W4 4AL, UK Tel +44 (0)20 8996 9001 Fax +44 (0)20 8996 7001 standards BSI - British Standards Institution BSI is the independent nati
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