1、DB61 jf f 1ft DB61/T 410-2007 2007-09-03 2007-09-03 I DB6ur 410-2007 *t;jfttJ Il1f:i.RA1:6.t 4t1:Il1HK 0 *ft$AR WA.WfrD. *.: $AWA. *ft.A; m*,*,*ffili, a. *tiftlnj(:ffl. ! ! t I I I t t I I I 1 i l DB6tff 410-2007 1 II *m .ffM*oo. mmfff*.o , 2 1115Im3t1tj: -fjitl)(fi: 00 *-.-:ilI1:i1*5mtr.J 51 flHffi
2、 J)!l *5moo t.kc Lff 8 W:I iJ 51 m)( IF, ltmIJfiM1.:riJ ft:J tP !IX1iTAOC:ffjm T *5m, rtffi, )jjJ*JRft.i6Jt;1) ixEfJ nEff1: 1iiJf1J:l3:i!)(1!I:ooliUTAOC*o L:fff8 Jt)j iJ 51 m)(1*, JtliiTAOC*Jfiffi T *;fm0 IFU 76 (2000 : iF1jffD-iFfi11iJg ATP 5-=M1r ADP 5-=J11J 6-PGDH. 6-MtJlmttll NADP+ 1:Ii;mUJ1pnt-
3、tr NADPH JJjjm:mJM;IJg(Wolnt=f.1l. U ji1tt:EI Fi/:, 1 25C iJf;Jmt.kf!l:-F 1fJ: minIU iJ.i5#J$tt1tJ 1 Jl mol iJwfPi A rJJD-6-.1ii1U: ;f115-.:.JlIHr (ADP) JJlA;J:0 illititj,Hl31M D-lii+ ATP . D-6-M.Hm. + ADP t 6-riiij)Uii (6-PGDH) iJftF8 I, D-6-.ijmHBz:mJf:liUHn1l7-;f1J. (NADP) 1t)tt)!l 5-.t.WIiij:fll
4、JJw)HtlM;H1i:JlInIl7-11. (NADPH)o 1JtA7g: 6-PGDH D-6-+ NADP+ . 5-. + NADPH + CO, + H t.tEZ.fY- NADPH Jf5J1J,8, 3 T D-ij,8,1$t. :ilI1:i1itEZ.Jlljjf5(1 340nm tlJPj-JiijtiIJl),fi!tiJE D-ijiJ,8,iio , 5.1 NADP: -15C -20C1f l 5.2 ATP: -15C -2oCHf1:1f I 5.3 MgSO, 7H10 DB61ff 410-2007 5.4 6-PGDH 5.5 1iiUfU;
5、 5.6 .=.ZM:1ni: 5. 7 Hel 5.8 NaOH 5.9 KOH 5. 10 :i:* 5. 11 tB 1.rem tlj,fb t*Uif 6 iii! 6. 1 l$1flm (5 mL, 1 mL 50-200 ilL, 10-50 II L) 6. 2 ltJIll; 1;:j22 U I 100J.1LIfF. 7.1. 3 1i1i1J!(.Ri i!1li.J;j26U 11. OmL !H. 7.1.4 He: 2 moIlL 7.1.5 NaOH: 2mollL. 7. 1. 6 KOH: mollL 7. 2 lit*J* 7.2.1 jff:it:,
6、7C,!, q:it1fii* X1m, x13, tpIi1*,fb, 1:itt1TtftWlIJ, 1tffll. OOOl. 800mUfHfftptfJCOl;:1*, fflNaOHmKOHmpH8ftti, tfPnfjd15min, 1tffll. OOO1. 800mL m:ittlJ0 7.2.4 fi,KOHmiaJpH8ftti, tfrfjFfjd15min, 1tffll. OOOl. 800mL m:itt1TtftW!1o 7. 3 J 01i 1 ilIff) 7.3.1 li:S;i;jlffi ? DB61IT 410-2007 ItHtilll, 1t1
7、JnA 1. OOOmL -=z.IJ$C!1k, 2.000 mL m:*, O. 020mL 6-flfj.Jlltfj, mii:l5min FnIJ(*tJ .2Omin), IJ1. r.twnii (*g 20min), IJfiijlfRj 2min mttJIJ 2min I*J I1IJ7.tJtttr7g : i ,. 3.040X 196.1 c=- XAAX f eX1. OOXO. 100X1000 5.961 I I c= XL1A X f A.rf1: C 1f4:f!5 IrffJIM.tf IJ I1*fQ , ljill: mL f *fffldiflJff
8、* d bElIll.IJYtf, lji1ll: em e NADPH IJmJt: tE 340 nm : 6.31 x mmor 1 x em- 9 D-IiIiJIJRtl.! Milit!1fi tEJtHfD-tin3t1M , itHfJ9H5!i%. 9. 1 19tmRlI.!/1t* fijifjf* 67mg D-tiiJWHifl, 72mg D-tiIt, :1.1O;.$IJ 100mL IJ.:F. JlljJ* (ffi 0.6gD-til1!/L). 9. 2 19tJ2im 9.2. 1 :tJomjflljJiJ2ii*, 9. 2. 2 _iitfM:i
9、i1:1Jr:iE:l:JiJ2i ;f4:mQ, :no O. 050mL IJm:nnjUtlYlm.i?ft, f4:mftAmttQ 9. 2. 4 19tlitiV (m* 2) :nu;. tE#ftIJm B ;f4: fiF1tE ;f4:+f*m z.f1P7t ;f4:m mm :I:f7.K 6-til1!IDiltftfj 1. OOOmL - - 2.000mL O.200mL 1. OOOmL O. 100mL - 1. 900mlmL O.020mL 1. OOOmL - O. 100mL 1. 900mL 0,020mL 1. OOOmL O.050mL O.050mL 1. 900mL 0.020mL mi?, *J6min*Il1Ytltm (AI) ,Jffi 7.3tJ;!JI fn tf 1U:jto 2 X .1 AIIJHiifr.1 A #/1, X 100 % . ,.,(3) .1 A.tiKi 4 DB611T 410-2007 , r 1 I I I t 1 I I I 1 1 I I I 5 1 I I
