1、DB611T 412-2007 2007-09-03 2007-09-03nffi 1 DB61 rr 412-2007 I 1 *flFft 9=t ffJ t:?kA1v:6t*i-tit0 *ft9=tAMDiliA.ili#Do *ft.m: 9=tAMiliAo *ftA: a*, *1, lM, *lfWo *flFftttijZt;1fl 0 DB61rr 412-2007 1 m:m *t;); m m! IE 7 rn fi-; mi(f mT tWt r:p trmnlJ WIE 0 2 ni!m: 11 i I ilt)i: itt: . , J rYI1:tfljcr:
2、p FfJ Jmi:1*;f;);(f:Efn 5 mITO T!Y;)J *;f;ff1tf.Jo fLH:tl: BWJRJ J lJ:ft1 t t-, JtMIJR pJf1JFfJ .(m*FfJ).umT*ff1,ITO,a*:$:ff1fi*mmT*;ff10 0 I FU 77 (200 1) -iitr:p itillJ RJ UJE EBC 9. 33-rumt:p1=t1lRJJE OWii7t) 3 *IUE:)t 3. 1 *tF-ltlflfiMi*PJlt: *, iiiljt, M, !l!tWi, *, 3. 2 f1!:j!Tlfillljifr!lIMl
3、PEP fW.f m-iJt p;j Ii! W PEP-CHA Wm-IW 5:; jAj Ii! WfjIlf z. fJ( 1i1;: L-LDH L-!LrutnJm1f NADH B-1W-M:RnIlt-tnw NAD mlliM:Tl1lt-ftr!t: U lfiifriIbfritdJIWFfJ11E1t1iJf jMiJi. 5. 5 L-finllStlt. 5. 6 ttfQiJi.;4?I.I 5.7HCI 5.8 NaOH 5.9 KOH 5.10 SlH!Ullf-tt!JI Fe (CN) dH 1 0 5. 11 i1tl$ ZnS047H20 5.12 m)
4、JpHi: #Fo. 66 g itmcitmT90mUj(rp, tjNaOHiJWjipIHlI3:?:7. 4. JE3:?:lOOmL 7.1.2 $m.1iUi/PHitr.J5Ur:t4?l.l: !+XlI mL ttrutFn.Kml.iFPez, mMlf. NADHt7mg: ATPl22mg: PEP-ClIMlllmg; nUAlmollLfJ MgSO, 7HO 4mL 2 DB61fT 412-2007 7. 1. 3 p;jIlilD., L-LDIMti.i.!4tlJ: J-H ftj, ImL 1!?li7.kif.Hfl1j!600 up;jllilmUi
5、I!Ff5J.5i j 7. 1. 8. 1 Carrez- I m ! 85mmollL Carrez- I m: ;f$lf: 3.60g K4Fe (CN) 63H 2 0, hui 100mL ;t1$17.kmM 0 7. 1. 8. 2 Carrez- II m 250mmol/LCaITez-IIm: lf: 7.20gZ N S0 4 7H 2 0 , hui 100ml,m:tm7.kmfUo liljf!ffla1, lthui Carrez- I m;fU Carrez-I1-m, JE7HP)J Carrez mo 7. 2 lit*itIJ. 7.2. 1 7C;ir
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7、, If:Mfflfft.o 7.2.3. 2 Jf;f.f.MA)JIi: mCarreziJifIJiiJJl#.f?f:J:j:JAtlB)JIi:, 25mL :i:.i1i1rtH.fJidff, hfli lOmL Carrez !;,);!j,n; 3000r/min !JiG 10min, JtlT:ff.f.IW1M,JVJ ! 1 HHv.k1rtJm: 25g fJ:j:J AtlH5Wi (1rtJm:t!JlilJIJK 50mL. i;J? , *ix:IJuA 1.00OmL tlTIlt!i;fIlW, 0.100mL t+rMftH, 1.900 mL :!I
8、lJ;.k, 0.010 mL :Ajli lPi, L-UlHn#It7-w, 1!iirif( 6min ffTi*ItX.,t1 12ff:Zl, (Aft 5lOmin), ttpl#ifx Q .7tN1l CAl) t+rThQ,l.1 1IJA (I) : 3 DB611T 412-2007 6A= (A J -A 2 ) 1l,lI,- (A J -A 2 ) !i!s .(l) liiJ5IJI!:E 365nm ragmiftr 0.500, n .ff.MtrMIJJl.o toAIttgIDllJmM smmt fft1t , line ffm. :m7j( 1fnJ.
9、1i L-.!JLJrnJmitli 1. 000 2.000 0.010 1. 000 o. 100 1. 900 0.010 ne, iW I!;ffsagI!it, .tlt-f1I/fW1to 8 it. *j1iH5t (2) it1HRJl: V,XMW C =:- X L1AX / .(2) e XdXV z X1000 trM:l:it1l:J1HIilrF05t 3.020X92.1 c ,- X LlAX / eX1.00 XO.100 X 1000 2.781 -XLlAX/ e C ttilfl (g/L #rfltlGfIl) CtJiIfI =-X100 (g/lO
10、Og) .(3) 4 DB61ff 412-2007 TWJIJ-1-,1y$iJ!JI! mL V 2 8.liJf-FitIJiJ!Jf-FrI1tfifiJ!J,f/.$:, F fftUfl1l 1 9. 2 tf.Jmzm I 9.2. 1 ;tml!Jt;flHlU:E.m. mL . f(!mlJ1 0.050mL tfJJD.i:t1JQJiJtiLiYlUm.g.tf, jzu)iHfllm, tJc;E f-F fu4io Jf!(1!, 7. 3tiLiVW ;fQ ;tltiLiYlIJ i!kfF0 2 X LlA 11Jl,+/lJilf/t LlA 11Jl, x 100%J .(4) LlA /lJi1fl 5 DB61/T 412-2007 11ft 1i A (f.!l.f111ft1i ) i1.IJi 1,.*.M.m.M,ffltr.AMtl, *. 2. llUmff 2-SC. )t:x1MllIJ:II:fJ1fflJlre*. 3,*.ffltts.mWfflo 5. -mjfttt&.jllYliJuttt:tjI-mijMPl. 6&-mm.m.*,.*nffm. 6
