1、BSI Standards PublicationWB11885_BSI_StandardCovs_2013_AW.indd 1 15/05/2013 15:06Soil quality - Effects of pollutants on juvenile land snails (Helicidae) - Determination of the effects on growth by soil contaminationBS EN ISO 15952:2018National forewordThis British Standard is the UK implementation
2、of EN ISO 15952:2018. It is identical to ISO 15952:2018. It supersedes BS EN ISO 15952:2011, which is withdrawn.The UK participation in its preparation was entrusted to Technical Committee EH/4, Soil quality.A list of organizations represented on this committee can be obtained on request to its secr
3、etary.This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. The British Standards Institution 2018 Published by BSI Standards Limited 2018ISBN 978 0 580 95877 9ICS 13.080.30Compliance with a British Standard cannot
4、 confer immunity from legal obligations.This British Standard was published under the authority of the Standards Policy and Strategy Committee on 31 July 2018.Amendments/corrigenda issued since publicationDate Text affectedBRITISH STANDARDBS EN ISO 15952:2018EUROPEAN STANDARDNORME EUROPENNEEUROPISCH
5、E NORMEN ISO 15952July 2018ICS 13.080.30 Supersedes EN ISO 15952:2011EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMIT EUROPEN DE NORMALISATIONEUROPISCHES KOMITEE FR NORMUNGCEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels 2018 CEN Ref. No. EN ISO 15952:2018: EAll rights of exploitation in
6、any form and by any means reserved worldwide for CEN national MembersSoil quality - Effects of pollutants on juvenile land snails (Helicidae) - Determination of the effects on growth by soil contamination (ISO 15952:2018)Qualit du sol - Effets des polluants vis-vis des escargots juvniles (Helicidae)
7、 - Dtermination des effets sur la croissance par contamination du sol (ISO 15952:2018)Bodenbeschaffenheit - Wirkungen von Schadstoffen auf Jungtiere von Landschnecken - Bestimmung der Wirkungen auf das Wachstum durch Bodenverunreinigung (ISO 15952:2018)This European Standard was approved by CEN on 1
8、5 June 2018.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be o
9、btained on application to the CEN-CENELEC Management Centre or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the
10、CEN-CENELEC Management Centre has the same status as the official versions.CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Irelan
11、d, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom.English VersionEN ISO 15952:2018 (E)European forewordThis document (EN ISO 15952:2018) has been prepared by Technical Comm
12、ittee ISO/TC 190 “Soil quality“ in collaboration with Technical Committee CEN/TC 444 “Test methods for environmental characterization of solid matrices” the secretariat of which is held by NEN.This European Standard shall be given the status of a national standard, either by publication of an identi
13、cal text or by endorsement, at the latest by January 2019, and conflicting national standards shall be withdrawn at the latest by January 2019.Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN shall not be held responsible for i
14、dentifying any or all such patent rights.This document supersedes EN ISO 15952:2011.According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Rep
15、ublic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United King
16、dom.Endorsement noticeThe text of ISO 15952:2018 has been approved by CEN as EN ISO 15952:2018 without any modification.iiBS EN ISO 15952:2018ISO 15952:2018Foreword ivIntroduction v1 Scope . 12 Normative references 13 Terms and definitions . 14 Principle 35 Test environment . 36 Reagents 36.3 Test s
17、ubstrate. 46.3.1 General 46.3.2 Artificial soil . 46.3.3 Natural soil 56.4 Feed. . 57 Apparatus . 57.1 Test containers. . 57.2 Feed containers. . 57.4 Balances. . 58 Storage and preparation of the samples 68.1 Soil to be tested 68.2 Waste material 69 Procedure. 69.1 Preparation of the test 69.1.1 Se
18、lection of the concentrations to be tested 69.1.2 Preparation of test mixtures 79.2 Distribution of test mixture . 89.3 Introduction of the feed. 89.4 Introduction of the biological reagent 89.5 Handling during the tests 89.5.1 General 89.5.2 Routine care . 99.5.3 Weekly task . 910 Reference substan
19、ce 1011 Calculations and expression of results .1011.1 Calculations . 1011.2 Expression of results . 1112 Validity of test .1313 Test report 13Annex A (informative) Static method 15Annex B (informative) Breeding technique for snails .16Annex C (informative) Example of composition of snail feed 21Ann
20、ex D (informative) Example of table of data 22Annex E (informative) Example of results with Helix aspersa aspersa 23Annex F (informative) Determination of the effects on growth by food contamination .27Annex G (informative) Suitable other snail species .30Bibliography .31 ISO 2018 All rights reserve
21、d iiiContents PageBS EN ISO 15952:2018ISO 15952:2018ForewordISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Eac
22、h member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the Internation
23、al Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the different
24、types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not
25、be held responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the document will be in the Introduction and/or on the ISO list of patent declarations received (see www.iso.org/patents).Any trade name used in this document is infor
26、mation given for the convenience of users and does not constitute an endorsement.For an explanation on the voluntary nature of standards, the meaning of ISO specific terms and expressions related to conformity assessment, as well as information about ISOs adherence to the World Trade Organization (W
27、TO) principles in the Technical Barriers to Trade (TBT) see the following URL: www.iso.org/iso/foreword.html.This document was prepared by Technical Committee ISO/TC 190, Soil quality, Subcommittee SC 4, Biological characterization.This second edition cancels and replaces the first edition (ISO 1595
28、2:2006), which has been technically revised.iv ISO 2018 All rights reservedBS EN ISO 15952:2018ISO 15952:2018IntroductionBecause of the limited amount of data available concerning toxicity of contaminants on soil organisms, the ecotoxicity of soils and waste are cause for serious concern at both nat
29、ional and international levels. Currently available tests use soil-fauna organisms restricted to annelid (earthworms and Enchytraeidae) and arthropod phyla (insects: Collembola and Coleoptera). Among the latter, two standards assess acute toxicity earthworms (ISO 11268-1) and coleoptera larvae6and t
30、hree other standards address sublethal effects of soil contaminants on reproduction (earthworms3, Collembola2, Enchytraeidae4). In the biological cycles of organisms, it appears that growth is, like reproduction, a fundamental ecophysiological parameter to be taken into consideration for the sustain
31、ability of species and ecosystems38.Snails are relevant ecological indicators for assessing the quality of soils(See References 1618 to 20323340 to 42), as they are characteristic of the soil surface layer (saprophagous and phytophagous) of which a large part of the biological cycle takes place in t
32、he soil (egg-laying, hatching, initial stages of development, hibernation, etc.)71829. During the other phases of their cycle, they eat soil and are in contact with the soil via their moist pedal sole (foot) covered with mucus and participate in the permanent exchanges with the soil (water, mineral
33、salts, excrement and finally shell and organic matter when they die)71831. In addition, they constitute an important link between plants, fauna and soil microorganisms. They correspond fully to the criteria for a good biological indicator: easy to sample and identify, they are widely distributed; th
34、ey accumulate contaminants (See References9,11 to 15,171822242930,33 to 48); their ecological and physiological characteristics are well-known71032; and they are now easy to breed under controlled conditions222632. Their susceptibility to common contaminants of their environment has been demonstrate
35、d (See References 11 to 16,19 to 28,30,33 to 38,37 to 48).This International Standard describes a method for determining the effects on survival and growth of young snails of substances, preparations (i.e. a mixture or solution composed of two or more substances), soils or waste materials added to a
36、n artificial or a natural soil. The described method is thus applicable to test contaminated soils or to compare different uncontaminated soils. The recommended species is Helix aspersa aspersa Mller (also commonly called: common garden snail, brown garden snail, garden snail, land snail, “Petit-Gri
37、s”; synonyms: Cantareus aspersus, Cornu aspersum56). Among land snails (stylommatophoran pulmonate gastropod molluscs of the Helicidae family), Helix aspersa aspersa Mller is the most ubiquitous. This palearctic species can be acclimated to regions with different types of climate: Mediterranean, oce
38、anic temperate, midcontinental temperate and even tropical. Helix aspersa aspersa Mller is of European origin and has been introduced into all parts of the world. They are now on all continents except Antarctica10.Indeed, in their natural environment, snails integrate the contaminants by contact (wi
39、th various substrates such as soil, soil leachates, plant litter), by ingestion (of plants and soil), as well as through the respiratory tract729. So, for specific testing purposes (evaluation of pesticide toxicity, for example), another test design, which is focussed on exposure via food uptake, is
40、 optionally available (Annex F and Reference 6). ISO 2018 All rights reserved vBS EN ISO 15952:2018This page deliberately left blankSoil quality - Effects of pollutants on juvenile land snails (Helicidae) - Determination of the effects on growth by soil contamination1 ScopeThis document specifies a
41、semi-static method for determining the effects of contaminants on growth and survival of young snails, usually Helix aspersa aspersa Mller. The animals are exposed via the cutaneous and digestive route using a test substrate (artificial or natural soil according to the objective of the study) to whi
42、ch defined amounts of the following are added: substances, mixtures or preparations; soils (contaminated or of unknown quality) or waste materials.This test takes into account the possible changes in the test substance, preparation, soil or waste material because the test mixtures are prepared and r
43、enewed every week during the 28-day test period.A static method may be implemented in addition to the semi-static method (optional). This method is described in Annex A.This method does not apply to substances for which the air/soil partition coefficient is greater than one, or to substances with va
44、pour pressure exceeding 300 Pa, at 25 C.2 Normative referencesThe following documents are referred to in the text in such a way that some or all of their content constitutes requirements of this document. For dated references, only the edition cited applies. For undated references, the latest editio
45、n of the referenced document (including any amendments) applies.ISO 10390, Soil quality Determination of pHISO 18400-206, Soil quality Sampling Part 206: Guidance on the collection, handling and storage of soil for the assessment of biological functional and structural endpoints in the laboratoryISO
46、 10694, Soil quality Determination of organic and total carbon after dry combustion (elementary analysis)ISO 11268-1, Soil quality Effects of pollutants on earthworms Part 1: Determination of acute toxicity to Eisenia fetida/Eisenia andreiISO 11269-2:2012, Soil quality Determination of the effects o
47、f pollutants on soil flora Part 2: Effects of contaminated soil on the emergence and early growth of higher plantsISO 11274, Soil quality Determination of the water-retention characteristic Laboratory methodsISO 11465, Soil quality Determination of dry matter and water content on a mass basis Gravim
48、etric methodEN 14735, Characterization of waste Preparation of waste samples for ecotoxicity tests3 Terms and definitionsFor the purposes of this document, the following terms and definitions apply.INTERNATIONAL STANDARD ISO 15952:2018 ISO 2018 All rights reserved 1BS EN ISO 15952:2018ISO 15952:2018
49、ISO and IEC maintain terminological databases for use in standardization at the following addresses: IEC Electropedia: available at http:/www.electropedia.org/ ISO Online browsing platform: available at https:/www.iso.org/obp3.1test substrateartificial soil or natural soil used as control and dilution substrate3.2matrixsoil or waste material under test3.3test mixturemixture of the test substance, preparation or matrix with the test
