1、 Collection of SANS standards in electronic format (PDF) 1. Copyright This standard is available to staff members of companies that have subscribed to the complete collection of SANS standards in accordance with a formal copyright agreement. This document may reside on a CENTRAL FILE SERVER or INTRA
2、NET SYSTEM only. Unless specific permission has been granted, this document MAY NOT be sent or given to staff members from other companies or organizations. Doing so would constitute a VIOLATION of SABS copyright rules. 2. Indemnity The South African Bureau of Standards accepts no liability for any
3、damage whatsoever than may result from the use of this material or the information contain therein, irrespective of the cause and quantum thereof. ISBN 978-0-626-22318-2 SANS 5815:2009Edition 2SOUTH AFRICAN NATIONAL STANDARD Pesticides Rearing and handling of the silver fish (Lepisma saccharina L.)
4、Published by SABS Standards Division 1 Dr Lategan Road Groenkloof Private Bag X191 Pretoria 0001Tel: +27 12 428 7911 Fax: +27 12 344 1568 www.sabs.co.za SABS SANS 5815:2009 Edition 2 Table of changes Change No. Date Scope Foreword This South African standard was approved by National Committee SABS S
5、C 1028F, Pesticides Laboratory facilities and rearing and handling of test animals, in accordance with procedures of the SABS Standards Division, in compliance with annex 3 of the WTO/TBT agreement. This document was published in February 2009. This document supersedes SABS SM 815:1978 (first editio
6、n). SANS 5815:2009 Edition 2 1 Pesticides Rearing and handling of the silver fish (Lepisma saccharina L.) 1 Scope This standard specifies a method for the rearing and handling of the silver fish (Lepisma saccharina L.). 2 Normative references The following referenced documents are indispensable for
7、the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. Information on currently valid national and international standards can be obtained from the SABS Standard
8、s Division. SANS 5808, Pesticides General conditions and equipment for the rearing and handling of test insects. 3 Rearing conditions Maintain the rearing conditions prescribed in SANS 5808. NOTE Silver fish thrive in humid conditions (i.e. a humidity of 70 %). 4 Equipment and material 4.1 Coarse sa
9、nd. 4.2 Containers, glass specimen tubes, 75 mm 25 mm. 4.3 Rearing containers and lids, type A rearing containers and type A rearing container lids as described in SANS 5808, or any other suitable round, square or rectangular containers. 5 Rearing media Use the following with (c) and (d) placed in t
10、he containers (see 4.2): a) coarse, unprinted pieces of paper covered with glue, and dried; b) pressed fibreboard panels kept damp by dripping a little water on them when necessary; c) starch; and SANS 5815:2009 Edition 2 2 d) cotton wool saturated with water. 6 Procedure 6.1 Spread 1 kg of the sand
11、 (see 4.1) on the bottom of a rearing container (see 4.3), and place the media (see clause 5) on it. The containers with media (5(c) and 5(d) are placed horizontally on the sand. 6.2 Place 100 freshly-emerged adult sliver fish in the rearing container. 6.3 Oviposition will take place at random in th
12、e rearing container. NOTE Silver fish lays eggs only twice a year. 6.4 The first generation adults will emerge after approximately six months. 6.5 Rear new colonies by repeating the procedure in 6.1 to 6.4 (inclusive). SABS SABS Standards Division The objective of the SABS Standards Division is to d
13、evelop, promote and maintain South African National Standards. This objective is incorporated in the Standards Act, 2008 (Act No. 8 of 2008). Amendments and Revisions South African National Standards are updated by amendment or revision. Users of South African National Standards should ensure that t
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