1、 Collection of SANS standards in electronic format (PDF) 1. Copyright This standard is available to staff members of companies that have subscribed to the complete collection of SANS standards in accordance with a formal copyright agreement. This document may reside on a CENTRAL FILE SERVER or INTRA
2、NET SYSTEM only. Unless specific permission has been granted, this document MAY NOT be sent or given to staff members from other companies or organizations. Doing so would constitute a VIOLATION of SABS copyright rules. 2. Indemnity The South African Bureau of Standards accepts no liability for any
3、damage whatsoever than may result from the use of this material or the information contain therein, irrespective of the cause and quantum thereof. ISBN 978-0-626-22307-6 SANS 5236:2009 Edition 2SOUTH AFRICAN NATIONAL STANDARD Bacteriostatic efficacy of water miscible liquids Published by SABS Standa
4、rds Division 1 Dr Lategan Road Groenkloof Private Bag X191 Pretoria 0001 Tel: +27 12 428 7911 Fax: +27 12 344 1568 www.sabs.co.za SABS This standard may only be used and printed by approved subscription and freemailing clients of the SABS.SANS 5236:2009 Edition 2 Table of changes Change No. Date Sco
5、pe Foreword This South African standard was approved by National Committee SABS TC 1022, Antiseptics, disinfectants and detergent-disinfectants, in accordance with procedures of the SABS Standards Division, in compliance with annex 3 of the WTO/TBT agreement. This document was published in April 200
6、9. This document supersedes SABS SM 236:1981 (first edition). This standard may only be used and printed by approved subscription and freemailing clients of the SABS.SANS 5236:2009 Edition 2 1 Bacteriostatic efficacy of water miscible liquids 1 Scope The standard describes a method to determine the
7、bacteriostatic efficacy of water miscible liquids. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including
8、any amendments) applies. Information on currently valid national and international standards can be obtained from the SABS Standards Division. SANS 171, Glassware and equipment for microbiological tests. SANS 636 (SABS 636), Disinfectants based on quaternary ammonium compounds. SANS 5553, Media and
9、reagents for microbiological tests. 3 Equipment Use equipment that is as specified in SANS 171. 4 Media and reagents 4.1 General Use ingredients for media and reagents of the quality specified in SANS 5553, and use the procedures specified in that method. 4.2 Nutrient agar Ingredients: agar 15,0 g b
10、eef extract 1,0 g peptone 5,0 g sodium chloride. 5,0 g yeast extract 2,0 g Dissolve the ingredients in water and dilute to 1 L. So adjust the pH value that, after sterilization, it will be 7,1. Dispense 15 mL volumes into bottles and sterilize by autoclaving. This standard may only be used and print
11、ed by approved subscription and freemailing clients of the SABS.SANS 5236:2009 Edition 2 2 5 Test organisms 5.1 Organisms Staphylococcus aureus Sta 10 ATCC 6538; Proteus vulgarisPre 6 CSIR 0030; Aerobacter aerogenesAer 5 ATCC 8329; and Bacillus subtilis .Bac 2 ATCC 6633. NOTE Additional organisms ma
12、y be used if so desired. 5.2 Preparation of test organism suspensions Maintain the test organisms and prepare the cultures for test suspensions as described in SANS 636. Using sterile water as the diluent, prepare suspensions as described in SANS 636, but containing (10 4 10) % living cells per mill
13、ilitre. 6 Test procedure 6.1 Using a sterile pipette, transfer 10 mL of the test sample to each of four sterile bottles having a nominal capacity of 30 mL and which are fitted with screw closures. 6.2 Transfer 0,1 mL of S. aureus test organism suspension (see 5.2) to each of the four bottles and mix
14、 well, avoiding the formation of air bubbles. Allow the bottles to stand at ambient temperature. 6.3 One hour after adding the test organism suspension, take two 1 mL volumes from one of the bottles and transfer each volume to a plate (Petri dish). For each separate plate take one 15 mL volume of nu
15、trient agar (see 4.2) which has been melted and tempered to 45 C, add it to the plate, and mix well to ensure an even distribution of colonies after incubation. Avoid spilling any of the contents of the plate during this process. Allow the agar to set, invert the plates, transfer them (suitably labe
16、lled) to an incubator, and incubate at 37 C. 6.4 After 48 h incubation, remove the plates from the incubator and count the colonies which have developed. Record these results. 6.5 Calculate the total count of S. aureus by averaging the counts on each plate. 6.6 Repeat the procedure in 6.3 to 6.5 (in
17、clusive) for each of the three remaining bottles after they have been allowed to stand at ambient temperature for 24 h, 7 d, and 28 d respectively. 6.7 Carry out a control test by transferring 10 mL of sterile water to a further screw-capped bottle. Transfer 0,1 mL of S. aureus test organism suspens
18、ion (see 5.2) to the bottle, mix well and immediately take two 1 mL volumes and transfer each volume to a plate (Petri dish). Pour the plates, mix, invert, incubate, count, and record the colonies developed as in 6.3 and 6.4. 6.8 Calculate the total control count of S. aureus as in 6.5. 6.9 Repeat t
19、he procedure in 6.1 to 6.8 (inclusive) using the remaining test organisms in turn. SABS This standard may only be used and printed by approved subscription and freemailing clients of the SABS. SABS Standards Division The objective of the SABS Standards Division is to develop, promote and maintain So
20、uth African National Standards. This objective is incorporated in the Standards Act, 2008 (Act No. 8 of 2008). Amendments and Revisions South African National Standards are updated by amendment or revision. Users of South African National Standards should ensure that they possess the latest amendmen
21、ts or editions. The SABS continuously strives to improve the quality of its products and services and would therefore be grateful if anyone finding an inaccuracy or ambiguity while using this standard would inform the secretary of the technical committee responsible, the identity of which can be fou
22、nd in the foreword. Tel: +27 (0) 12 428 6666 Fax: +27 (0) 12 428 6928 The SABS offers an individual notification service, which ensures that subscribers automatically receive notification regarding amendments and revisions to South African National Standards. Tel: +27 (0) 12 428 6883 Fax: +27 (0) 12
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25、 an individual updating service called INFOPLUS, which ensures that subscribers automatically receive notification regarding amendments to, and revisions of, international standards. Tel: +27 (0) 12 428 6666 Fax: +27 (0) 12 428 6928 E-mail: infosabs.co.za Copyright The copyright in a South African N
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27、sion. This does not preclude the free use, in the course of implementing the standard, of necessary details such as symbols, and size, type or grade designations. If these details are to be used for any purpose other than implementation, prior written permission must be obtained. Details and advice can be obtained from the Senior Manager. Tel: +27 (0) 12 428 6666 Fax: +27 (0) 12 428 6928 E-mail: infosabs.co.za This standard may only be used and printed by approved subscription and freemailing clients of the SABS.
